
蚯蚓处理牛粪效果研究
Study on the Effect of Earthworm Treatment on Cattle Manure
为解决宁南山区农村畜禽粪便造成的环境卫生和土壤污染问题,本研究利用“大平二号”蚯蚓处理牛粪,采用室内分析方法了解蚯蚓摄食牛粪秸秆混合物的效果。结果表明,牛粪经蚯蚓处理后蚓粪质地疏松、颗粒明显、无臭味。蚓粪容重平均为0.39 g/cm3,总孔隙度、毛管孔隙度和非毛管孔隙度平均为85.85%、62.86%、22.99%,透气性和通水性明显改善。pH、水溶性盐总量降低,性质趋于中性。相比鲜牛粪,有机质、全氮、全钾含量及碳氮比分别减少了62.6%~69.8%、41.1%~49.0%、20.7%~33.2%和34.3%~47.8%,全磷含量增加了1.3~2.0倍,添加适量玉米秸秆有助于蚓粪有机肥品质提升。蚯蚓处理加速了牛粪中纤维素、半纤维素和木质素的分解,分别较鲜牛粪减少了74.0%~77.5%,65.6%~83.1%,5.2%~16.3%。蚯蚓处理对Cu、Zn去除效果好,对Pb、Cr的去除效果不佳。蚯蚓对牛粪物料转化效率为43.42%~65.15%,添加玉米秸秆可以提升转化效率。当地养殖户可利用蚯蚓对牛粪等畜禽粪便进行转化,提升粪污综合利用率,减少环境污染。
In order to solve the environmental sanitation and soil pollution caused by livestock manure in southern Ningxia, this study used “Daping 2” earthworm to treat cattle manure, and then explored the effect of earthworms feeding on mixture of cattle manure and corn stalk in different proportions by laboratory measurements. The results showed that vermicompost was loose, had distinct granular structure and no bad smell. The average bulk density of vermicompost was 0.39 g/cm3. The average total porosity, capillary porosity and non-capillary porosity were 85.85%, 62.86% and 22.99%, respectively. The air permeability and water permeability of vermicompost were obviously improved. Total water soluble salt and pH decreased, and their properties tended to be neutral. Compared with fresh cattle manure, contents of organic matter, total nitrogen, total potassium and C/N decreased by 62.6%-69.8%, 41.1%-49.0%, 20.7%-33.2%, 34.3%-47.8% respectively, while content of total phosphorus increased by 1.3-2.0 times. Adding appropriate amount of corn stalk helped to improve the quality of vermicompost organic fertilizer. Earthworm treatment accelerated the decomposition of cellulose, hemicellulose and acid detergent ligninin of cattle manure, their contents decreased by 74.0%-77.5%, 65.6%-83.1% and 5.2%-16.3% respectively. The removal efficiency of Cu, Zn by the treatment was better than that of Pb and Cr. The conversion efficiency of cattle manure by earthworms was 43.42%-65.15%, and the conversion efficiency could be improved by adding corn stalk. Local farmers can use earthworms to transform livestock and poultry manure such as cattle dung, improve the comprehensive utilization rate of manure and reduce environmental pollution.
蚯蚓 / 牛粪 / 畜禽粪便 / 玉米秸秆 / 蚓粪 {{custom_keyword}} /
earthworm / cattle manure / livestock manure / corn stalk / vermicompost {{custom_keyword}} /
[1] |
姚升, 王光宇. 基于分区视角的畜禽养殖粪便农田负荷量估算及预警分析[J]. 华中农业大学学报, 2016(1):72-84.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[2] |
吴浩玮, 孙小淇, 梁博文, 等. 我国畜禽粪便污染现状及处理与资源化利用分析[J]. 农业环境科学学报, 2020, 39(6):1168-1176.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[3] |
黄亚捷, 崔艳智, 贾小梅, 等. “十四五”期间宁夏畜禽养殖污染趋势与污染防治对策研究[J]. 中国农学通报, 2022, 38(32):78-85.
为了评估“十四五”期间宁夏高速发展的畜禽养殖业带来的污染及对环境承载压力的影响,基于宁夏畜禽养殖数据,采用排泄系数法估算了化学需氧量(COD)、氮磷产生情况,在此基础上计算了畜禽粪便的耕地负荷。基于计算畜禽养殖环境适宜性、土地承载力及养分承载力,评估“十四五”期间将面临的环境承载压力。结果发现:(1)全区畜禽养殖所带来的COD、氮、磷排放量分别为123万、6.86万、7440 t。(2)从全区的环境承载压力来看,全区畜禽养殖环境适宜性区域主要分布在南部山区。各市畜禽粪污土地承载能力均大于畜禽养殖量,而利通区养殖量是粪污土地承载能力的3.1倍,超出当地土地承载能力,养殖污染风险极大。银川市区、利通区及泾源县无法实现粪肥全部还田,养分承载压力大。(3)“十四五”期间,畜禽养殖量逐年增加,在2025年氮排放量达12万t,比2020年增加了5万t;磷及COD排放量达1.2万、251.7万t,分别是2020年的1.7、1.5倍。在2025年耕地负荷预警值更是达到了3.50的高度,因此从适当控制养殖规模、严格执行生态消纳、加强粪污肥料化利用、科学治污、粪污集中处理中心建设及政策扶持6个方面提出对策建议。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[4] |
汪开英, 吴捷刚, 赵晓洋. 畜禽场空气污染物检测技术综述[J]. 中国农业科学, 2019, 52(8):1458-1474.
随着畜禽养殖集约化程度的提高,高密度、集约化的畜禽养殖引起的畜禽养殖场空气污染问题日益突出,畜牧业已成为我国空气污染的重要来源之一。畜禽场排放的氨气(NH<sub>3</sub>)、硫化物(H<sub>2</sub>S)、颗粒物(particulate matters,PM)和挥发性有机物(volatile organic compounds,VOCs)等空气污染物不仅对畜禽场人畜健康造威胁,还会扩散到周围环境中形成大气污染。科学适用的空气污染物检测方法和技术是研究和控制畜禽场空气污染的前提。文章对国内外畜禽场的NH<sub>3</sub>、H<sub>2</sub>S、温室气体、PM以及恶臭等主要空气污染物的检测方法与技术进行了介绍与分析。以NH<sub>3</sub>、H<sub>2</sub>S为代表的畜禽场有害气体检测方法主要有化学分析法、半导体气体传感器检测法、光谱法、质谱法与气相色谱法,其中,湿化学法灵敏度高,成本低,可以准确获取气体浓度,但检测费时费力,且无法实时在线检测;气体检测管法成本较低,操作简单,但误差相对较大;电化学传感器法灵敏度较高,成本适中,可以连续检测气体浓度,但存在装置易老化、使用寿命较短等缺陷;光谱法、质谱法以及气相色谱法可快速准确检测气体,但检测仪器昂贵,使用成本较高,不适合生产性畜禽场的常规检测。文章主要介绍与分析了反刍动物肠道发酵、畜禽舍、粪污贮存与处理过程中温室气体CH<sub>4</sub>、CO<sub>2</sub>、N<sub>2</sub>O的检测方法和技术。畜牧业温室气体排放随时间、季节和采样点等不同而不断变化,实现畜牧业温室气体的精确监测较为困难,目前仍未有国际通用的检测方法与测量标准,畜牧业温室气体检测方法与技术及其标准研究亟待开展。文章从物理特征、化学特征及生物特征三个方面对畜禽场排放的颗粒物检测方法与技术进行了综述。畜禽场PM成分复杂,其吸附的NH<sub>3</sub>、恶臭化合物、微生物的影响有待评估,因此亟待完善PM的物理、化学和生物成分的检测方法与技术,以获取畜禽场PM的全面特征信息。文章对畜禽场的主要恶臭物质成分和恶臭感官的检测方法与技术进行了综述。相比气相色谱-质谱联用法,采用专业嗅辨人员对恶臭浓度进行嗅辨分析的方法成本更高,且主观性较强。但气相色谱-质谱联用技术无法仅凭借气体样品确定所有气态有机化合物,同时难以确定引起恶臭的化合物种类。因此结合气相色谱与动态嗅觉仪可更全面的分析恶臭样品指标。文章还对国内外畜禽场空气污染物(包含有害气体、温室气体、颗粒物以及恶臭物质)的主要检测方法与技术进行综述,以期为我国畜禽养殖业的空气污染物检测技术的研发与应用提供参考。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[5] |
吴根义, 廖新俤, 贺德春, 等. 我国畜禽养殖污染防治现状及对策[J]. 农业环境科学学报, 2014, 33(7):1261-1264.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[6] |
温凌嵩, 宋立华, 臧一天, 等. 蚯蚓处理畜禽粪便研究进展[J]. 家畜生态报, 2020, 41(7):85-89.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[7] |
柏彦超, 周雄飞, 汪孙军, 等. 牛粪经蚯蚓消解前后理化性质的比较研究[J]. 江西农业学报, 2010, 22(10):135-137.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[8] |
鲁耀雄, 崔新卫, 陈山, 等. 牛粪预处理对蚯蚓堆肥生物学特性和养分含量的影响[J]. 江西农业学报, 2019, 31(4):39-45.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[9] |
庞军柱, 乔玉辉, 孙振钧, 等. 蚯蚓对麦秸分解速率的影响及其对氮矿化的贡献[J]. 生态学报, 2009, 29(2):1017-1023.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[10] |
林嘉聪. 蚯蚓堆肥物料特性与蚯蚓-蚯蚓粪分离技术研究[D]. 武汉: 华中农业大学, 2021
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[11] |
李辉信, 胡锋, 仓龙, 等. 蚯蚓堆制处理对牛粪性状的影响[J]. 农业环境科学学报, 2004(3):588-593.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[12] |
赵海涛, 狄霖, 刘平, 等. 蚯蚓生物床工程处理对牛粪性质的影响[J]. 农业工程学报, 2011, 27(9):255-259.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[13] |
周颖. 秸秆与牛粪蚯蚓堆制处理时纤维素、半纤维素和木质素降解率及相关酶活性变化的研究[D]. 杨凌: 西北农林科技大学, 2009.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[14] |
王华为, 吉莉, 朱莲, 等. 蚯蚓对不同比例的秸秆与牛粪混合物的消解转化效果[J]. 中国农技推广, 2015, 31(12):37-40.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[15] |
王峰, 缪丽娟, 张明月, 等. 牛粪矿物混合蚯蚓堆肥中堆制物Pb和Cd吸附性能变化[J]. 农业工程学报, 2021, 37(6):197-204.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[16] |
缪丽娟, 张炜文, 王峰, 等. 牛粪及其蚓粪基本理化性状及重金属形态分布差异[J]. 杭州师范大学学报(自然科学版), 2020, 19(5):488-495.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[17] |
邢宇翚, 李云乐, 辛乐阳, 等. 蚯蚓对有机肥(牛粪)中铬镉铅金属富集作用的研究[J]. 北京农学院学报, 2015, 30(1):55-63,85.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[18] |
敬佩, 李光德. 蚯蚓诱导对土壤中铅镉形态的影响[J]. 水土保持学报, 2009, 23(3):66-68.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[19] |
全国农业技术推广服务中心, 中国农业科学院农业资源与农业区划研究所, 山东省土壤肥料总站. NY/T 1121.16—2006,土壤检测第16部分:土壤水溶性盐总量的测定[S]. 北京: 中国标准出版社, 2006:1-4.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[20] |
中国科学院南京土壤研究所. 土壤理化分析[M]. 上海: 上海科学技术出版社,1983.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[21] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[22] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[23] |
The white rot basidiomycete Phanerochaete chrysosporium completely degrades lignin and a variety of aromatic pollutants during the secondary metabolic phase of growth. Two families of secreted heme enzymes, lignin peroxidase (LiP) and manganese peroxidase (MnP), are major components of the extracellular lignin degradative system of this organism. MnP and LiP both are encoded by families of genes, and the lip genes appear to be clustered. The lip genes contain eight or nine short introns; the mnp genes contain six or seven short introns. The sequences surrounding active-site residues are conserved among LiP, MnP, cytochrome c peroxidase, and plant peroxidases. The eight LiP cysteine residues align with 8 of the 10 cysteines in MnP. LiPs are synthesized as preproenzymes with a 21-amino-acid signal sequence followed by a 6- or 7-amino-acid propeptide. MnPs have a 21- or 24-amino-acid signal sequence but apparently lack a propeptide. Both LiP and MnP are regulated at the mRNA level by nitrogen, and the various isozymes may be differentially regulated by carbon and nitrogen. MnP also is regulated at the level of gene transcription by Mn(II), the substrate for the enzyme, and by heat shock. The promoter regions of mnp genes contain multiple heat shock elements as well as sequences that are identical to the consensus metal regulatory elements found in mammalian metallothionein genes. DNA transformation systems have been developed for P. chrysosporium and are being used for studies on gene regulation and for gene replacement experiments.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[24] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[25] |
王海磊, 李宗义. 三种重要木质素降解酶研究进展[J]. 生物学杂志. 2003, 20(5):9-11.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[26] |
刘鹏. 利用蚯蚓对不同配比牛粪和玉米秸秆堆肥效果的影响[J]. 中国畜禽种业, 2022, 18(6):37-39.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[27] |
王海候, 何胥, 陶玥玥, 等. 添加不同粒径炭基辅料改善猪粪好氧堆肥质量的效果[J]. 农业工程学报, 2018, 34(9):224-232.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[28] |
陈学民, 王惠, 伏小勇, 等. 赤子爱胜蚓处理污泥对其性质变化的影响[J]. 环境工程学报, 2010, 4(6):1421-1425.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[29] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[30] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
{{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
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