
梨果皮色素含量的测定方法研究
Study on Determination Method of Pigment Content of Pear Peel
为找到适用于不同梨果皮颜色色素含量的测定方法,采用2种测定方法对‘新梨7号’‘秋月’‘鸭梨’‘玉露香’‘香红梨’5个不同果皮颜色的梨品种进行叶绿素和总类胡萝卜素含量的测定。结果表明,对于‘新梨7号’和‘玉露香’,80%丙酮浸提法和95%乙醇浸提法测得的总叶绿素含量、叶绿素a含量、叶绿素b含量和总类胡萝卜素含量均差异不显著。对于‘鸭梨’‘香红梨’‘秋月’,80%丙酮浸提法和95%乙醇浸提法测得的总类胡萝卜素含量差异不显著,但80%丙酮浸提法测得的总叶绿素含量、叶绿素a含量和叶绿素b含量显著高于95%乙醇浸提法测得的含量。80%丙酮浸提法适用于测定所有品种梨果皮的叶绿素含量和总类胡萝卜素含量。测定果皮颜色为绿色和绿色为底色有红晕的梨果皮叶绿素和总类胡萝卜素时,可优先选择95%乙醇浸提法。若只需测定总类胡萝卜素含量,所有果皮颜色的梨均可选择95%乙醇浸提法。
The paper aims to find a method suitable for determining the color pigment content of different pear peels. The chlorophyll content and total carotenoid content were determined for five pear varieties with different peel colors, namely ‘Xinli No.7’, ‘Akizuki’, ‘Yali’, ‘Yuluxiang’ and ‘Xianghongli’ by two methods. The results showed that for ‘Xinli No.7’ and ‘Yuluxiang’, the total chlorophyll content, chlorophyll a content, chlorophyll b content and total carotenoid content measured by the 80% acetone extraction method and the 95% ethanol extraction method weren’t significantly different. For ‘Yali’, ‘Xianghongli’ and ‘Akizuki’, the total carotenoid content measured by the 80% acetone extraction method and the 95% ethanol extraction method wasn’t significantly different, but the total chlorophyll content, chlorophyll a content and chlorophyll b content measured by the 80% acetone extraction method were significantly higher than the content measured by the 95% ethanol extraction method. The chlorophyll content and total carotenoid content of pear peels of all varieties can be determined by 80% acetone extraction. When determining the chlorophyll and total carotenoids of pear peel with green color and green as the background color with red halo, the 95% ethanol extraction method can be preferred. If the total carotenoid content is only required, all peel colors can be extracted with 95% ethanol.
梨 / 果皮 / 叶绿素 / 类胡萝卜素 / 测定方法 {{custom_keyword}} /
pear / pericarp / chlorophyll / carotenoids / test methods {{custom_keyword}} /
[1] |
何婉琳, 施露, 李亚辉, 等. 梨果实品质及其影响因素研究进展[J]. 浙江农业科学, 2022, 63(2):330-333.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[2] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[3] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[4] |
施泽彬. 砂梨果皮性状形成机制研究[D]. 南京: 南京农业大学, 2011.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[5] |
苏正淑, 张宪政. 几种测定植物叶绿素含量的方法比较[J]. 植物生理学通讯, 1989(5):77-78.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[6] |
贺倩, 杨文杰, 张旭娟, 等. 植物叶绿素快速浸提法的条件优化[J]. 江苏农业科学, 2013, 41(11):97-98,138.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[7] |
洪法水, 魏正贵, 赵贵文. 菠菜叶绿素的浸提和协同萃取反应[J]. 应用化学, 2001(7):532-535.
有机溶剂;菠菜叶绿素的浸提和协同萃取反应
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[8] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[9] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[10] |
徐芬芬, 叶利民, 徐卫红, 等. 小白菜叶绿素含量的测定方法比较[J]. 北方园艺, 2010(23):32-34.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[11] |
刘丹. LED光源对花生以及黄瓜幼苗生长的影响[D]. 南京: 南京农业大学, 2013.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[12] |
袁自然, 叶寅, 武际, 等. 基于高光谱成像技术的辣椒叶片叶绿素含量估算[J]. 江苏农业科学, 2021, 49(16):189-193.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[13] |
向珊珊. 木鳖果假种皮类胡萝卜素的提取纯化及其纳米粒的制备[D]. 北京: 北京林业大学, 2019.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[14] |
沈波, 楼志华, 谢小波, 等. HPLC测定网纹甜瓜类胡萝卜素含量方法研究[J]. 北方园艺, 2009(11):54-56.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[15] |
陈敏氡, 朱海生, 温庆放, 等. UPLC测定草莓果实中类胡萝卜素含量[J]. 果树学报, 2013, 30(4):706-711.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[16] |
王彬, 林亮, 陈敏氡, 等. 南瓜类胡萝卜素含量的超高效液相色谱分析[J]. 农学学报, 2017, 7(12):22-27.
类胡萝卜素是一类重要的天然色素。为了分析南瓜类胡萝卜素的成分及含量,以南瓜果肉为材料,通过对不同提取剂(甲醇、乙醇、石油醚、丙酮、丙酮-石油醚(1:1)、丙酮-石油醚(1:2)、丙酮-石油醚(1:4))和提取方法(皂化和非皂化)的筛选,优化南瓜果肉类胡萝卜素的提取条件,并应用超高效液相色谱(UPLC)技术分析了23 个南瓜品种的类胡萝卜素成分及含量。结果表明,以乙醇作为提取剂并通过皂化处理更适合南瓜果肉类胡萝卜素的提取。南瓜果肉中含有β-胡萝卜素、α-胡萝卜素和叶黄素3 种类胡萝卜素,其成分组成及含量在南瓜种内及种间均存在差异,大部分印度南瓜和中国南瓜品种的类胡萝卜素总量明显高于美洲南瓜品种。实验所优化的提取及测定方法适合南瓜果肉类胡萝卜素的定性定量分析。
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[17] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[18] |
再吐娜·买买提, 许建, 姚军, 等. 甜瓜果实发育过程中叶绿素和类胡萝卜素含量的变化[J]. 中国瓜菜, 2021, 34(8):44-48.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[19] |
李伟, 李正洙, 曲柏宏. 不同套袋期对苹果梨果皮色素含量的影响[J]. 湖南农业科学, 2010(15):34-35.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[20] |
于年文, 李俊才, 王家珍, 等. 套袋对南果梨果皮色素含量的影响[J]. 西北农业学报, 2012, 21(9):103-107.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[21] |
李俊才, 李宝江, 于丹, 等. 2个西洋梨红色芽变品种与原品种果实发育期果皮色素变化比较研究[J]. 中国果树, 2010(1):27-30.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[22] |
周金鑫. 砂梨果实石细胞含量和果皮色泽差异的比较分析[D]. 扬州: 扬州大学, 2020.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[23] |
俞波. 红色砂梨花青苷生物合成相关基因分离及表达研究[D]. 杭州: 浙江大学, 2012.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[24] |
王龙, 李鸿雁, 杨健, 等. ‘红香酥’梨果皮色素变化规律及套袋对其形成的影响[J]. 果树学报, 2012, 29(3):333-337.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
[25] |
史梦琪, 李慧, 徐凯, 等. 套袋对“黄花”梨果实外观品质的影响[J]. 中国南方果树, 2019, 48(1):100-106.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
{{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
/
〈 |
|
〉 |