2种花色的薰衣草花色苷成分分析

赖刚刚, 李敏, 蒋新明, 徐盼盼, 李子玉, 郭丹丽

中国农学通报. 2023, 39(15): 39-45

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中国农学通报 ›› 2023, Vol. 39 ›› Issue (15) : 39-45. DOI: 10.11924/j.issn.1000-6850.casb2022-0630
林学·园艺·园林

2种花色的薰衣草花色苷成分分析

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Anthocyanin in Lavender of Two Flower Colors: Analysis of Compositions

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摘要

为明确2种不同花色薰衣草间花色苷成分及含量差异性,探索薰衣草花色与花色苷的相关性。以‘新薰二号’(蓝花)和‘YXA-05’(白花)2种薰衣草花器官为研究材料,利用超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)联用技术测定2种薰衣草花器官中花色苷的成分及含量。结果表明,‘新薰二号’和‘YXA-05’2种薰衣草花冠中总花色苷含量分别为42.24 μg/g和35.96 μg/g,均含有6类花色苷,分别为矢车菊素、芍药花素、飞燕草素、矮牵牛素、锦葵素和天竺葵素。其中‘新薰二号’含有39种花色苷,‘YXA-05’含有37种花色素苷,‘新薰二号’含有6种‘YXA-05’中不存在的花色苷,而‘YXA-05’含有4种‘新薰二号’中不存在的花色苷。可见,2种薰衣草中花色苷的种类丰富,且成分及含量存在差异,薰衣草花冠的颜色与其花色素苷的成分和含量有关。

Abstract

The aim is to clarify the composition and content differences of anthocyanin between two kinds of lavender varying in flower color, and to explore the relevance between lavender flower color and anthocyanin. The flower organs of ‘Xinxun No. 2’ (blue flower) and ‘YXA-05’ (white flower) were used as research materials. The composition and content of anthocyanins in flower organs of the two lavender varieties were determined by using the combination technology of ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The result showed that the total anthocyanin content in ‘Xinxun No. 2’ and ‘YXA-05’ was 42.24 μg/g and 35.96 μg/g respectively, and both two varieties contained 6 types of anthocyanin, including cyanidin, peonidin, delphinidin, petunidin, malyidin and pelargonidin. There were 39 types of anthocyanin in ‘Xinxun No. 2’, and 37 types of anthocyanin in ‘YXA-05’. ‘Xinxun No. 2’ contained 6 types of anthocyanin that were not found in ‘YXA-05’, while ‘YXA-05’ contained 4 types of anthocyanin that were not found in ‘Xinxun No. 2’. It was indicated that in these two lavender varieties, anthocyanin was rich in types and differed in composition and content. The flower color of corolla was related to the composition and content of its anthocyanin.

关键词

超高效液相色谱-四极杆-飞行时间质谱 / ‘新薰二号’ / 花色苷 / 飞燕草素 / 矮牵牛素

Key words

ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry / ‘Lavender Xinxun No. 2’ / anthocyanin / delphinidin / petunidin

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赖刚刚 , 李敏 , 蒋新明 , 徐盼盼 , 李子玉 , 郭丹丽. 2种花色的薰衣草花色苷成分分析. 中国农学通报. 2023, 39(15): 39-45 https://doi.org/10.11924/j.issn.1000-6850.casb2022-0630
LAI Ganggang , LI Min , JIANG Xinming , XU Panpan , LI Ziyu , GUO Danli. Anthocyanin in Lavender of Two Flower Colors: Analysis of Compositions. Chinese Agricultural Science Bulletin. 2023, 39(15): 39-45 https://doi.org/10.11924/j.issn.1000-6850.casb2022-0630

参考文献

[1]
KIVRAK S. Essential oil composition and antioxidant activities of eight cultivars of lavender and lavandin from western anatolia[J]. Ind. crop. prod, 2018, 117:88-96.
[2]
JADCZAK P, KULPA D, PRZEWODOWSKI W. Correction to: positive effect of AgNPs and AuNPs in in vitro cultures of Lavandula angustifolia Mill[J]. Plant cell, tissue and organ culture (PCTOC), 2021, 146(1):213-213.
A correction to this paper has been published: https://doi.org/10.1007/s11240-021-02087-2
[3]
陈和平, 周贺新, 冉权善, 等. 薰衣草的研究进展[J]. 农垦医学, 2005, 27(2):142-145.
[4]
胡彬, 丁德辉, 冯超, 等. 钟冠报春苣苔花发育进程中花色素苷含量的变化[J]. 园艺学报, 2018, 45(1):117-125.
采用超高效液相色谱—四级杆飞行时间串联质谱联用技术(UPLC-Q-TOF-MS)测定钟冠报春苣苔(Primulina swinglei)花发育进程中花色素苷在各花器官组织中的分布、含量及比例。共鉴定出17种花色素苷类物质,分别为矢车菊素、芍药花素、飞燕草素、矮牵牛素及锦葵素及其与葡萄糖、芸香糖、乙酰芸香糖等糖苷组合而成的苷元。研究发现,呈紫色的花瓣中蓝紫色的花色素(飞燕草素、矮牵牛素及锦葵素)比例达到64.0%;呈红色的花筒中红粉色的矢车菊素及芍药花素分别占36.7%和20.0%。开花前总花色素苷含量缓慢上升并趋于稳态,该过程中红粉色的花色素占据比例逐渐减小,而蓝紫色花色素所占比例渐趋增加,这可能与花瓣/花筒的比例渐趋增大有关。而花盛开之后,花色素苷含量急剧降低,可能与花色素苷的降解或稀释相关。
[5]
张中华, 张会灵, 李宁, 等. MYB基因调控植物花青素合成的研究进展[J/OL]. 分子植物育种,1-24[2022-01-12]. http://kns.cnki.net/kcms/detail/46.1068.S.20210709.0900.006.html.
[6]
潘新怡, 史昆, 王赞, 等. 紫花苜蓿花青苷合成的转录组分析[J]. 草地学报, 2021, 29(5):866-875.
为探究紫花苜蓿(Medicago sativa)花色形成的分子机制,本试验以紫花苜蓿及其白花突变体为研究材料,进行转录组测序。结果表明:紫花和白花之间共有差异表达基因4 857个;代谢通路富集分析中,苯丙氨酸生物合成途径等6条代谢通路显著富集;研究还发现类黄酮合成通路中关键基因二氢黄酮醇4-还原酶(Dihydroflavonol 4-reductase,DFR)在白花突变体中的表达量显著下调,其启动子中存在MYB和bHLH等转录因子的结合位点;转录组以及荧光定量PCR的结果均表明:MYB,bHLH和WD40等基因的表达量都具有显著性差异。这些结果表明MYB转录因子或MBW复合物可能通过调控DFR的转录影响紫花苜蓿花色形成。本试验为探明紫花苜蓿花青苷合成的分子机制提供了理论基础。
[7]
周萍, 吴仲君, 郑洁, 等. 花色苷提取及纯化研究进展[J]. 精细化工, 2020, 37(8):1513-1523.
[8]
MA H, JOHNSON S L, LIU W X, et al. Evaluation of polyphenol anthocyanin-enriched extracts of blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry for free radical scavenging, reactive carbonyl species trapping, anti-glycation, anti-amyloid aggregation, and microglial neuroprotective effects[J]. International journal of molecular sciences, 2018, 19(2):461.
[9]
YOON B I, BAE W J, CHOI Y S, et al. Anti-inflammatory and antimicrobial effects of anthocyanin extracted from black soybean on chronic bacterial prostatitis rat model[J]. Chinese journal of integrative medicine, 2018, 24(8):621-626.
To investigated the anti-inflammatory and antimicrobial effects of anthocyanins extracted from black soybean on the chronic bacterial prostatitis (CBP) rat model.The Sprague-Dawley rats were divided into 4 groups, including control, ciprofloxacin, anthocyanins and anthocyanins with ciprofloxacin groups (n=8 in each group). Then, drip infusion of bacterial suspension (Escherichia coli Z17 O:K:H) into Sprague-Dawley rats was conducted to induce CBP. In 4 weeks, results of prostate tissue, urine culture, and histological analysis on the prostate were analyzed for each group.The use of ciprofloxacin, anthocyanins, and anthocyanins with ciprofloxacin showed statistically significant decreases in bacterial growth and improvements in the reduction of prostatic inflammation compared with the control group (P<0.05). The anthocyanins with ciprofloxacin group showed a statistically significant decrease in bacterial growth and improvement in prostatic inflammation compared with the ciprofloxacin group (P<0.05).These results suggest that anthocyanins may have anti-inflammatory and antimicrobial effects, as well as a synergistic effect with ciprofloxacin. Therefore, we suggest that the combination of anthocyanins and ciprofloxacin may be effective in treating CBP to obtain a higher rate of treatment success.
[10]
薛宏坤, 谭佳琪, 刘钗, 等. 蓝莓果渣花色苷提取工艺优化及其提取物的抗肿瘤活性[J]. 精细化工, 2019, 36(9):1881-1890.
[11]
KRGA I, MILENKOVIC D. Anthocyanins: from sources and bioavailability to cardiovascular-health benefits and molecular mechanisms of action[J]. Journal of agricultural and food chemistry, 2019, 67(7):1771-1783.
Anthocyanins are phytochemicals widely found in plant foods, with berries and fruit-derived beverages as the main dietary sources. Accumulating evidence suggests the positive role of anthocyanins in preserving cardiovascular health. Epidemiological data show an association between anthocyanin intake and lower risk of myocardial infarction and cardiovascular-disease-related mortality. Clinical studies report the beneficial effects of the consumption of different anthocyanin-rich sources on surrogate markers of cardiovascular risk. Animal and in vitro evidence suggest the protective role of anthocyanins in dysfunctions related to the development of cardiovascular diseases. Still, the underlying molecular mechanisms of anthocyanin action seem complex and are not entirely clear. This review aims to give a comprehensive update on anthocyanins and their cardioprotective properties. It provides information on their sources; quantities consumed through diet; absorption; bioavailability; cardiovascular properties; and underlying mechanisms of action, including their effects on gene and protein expression and their interactions with cell-signaling pathways and miRNAs.
[12]
刘春玉, 李敏, 郭丹丽. 高效液相色谱法测定薰衣草花中花色苷含量[J]. 分子植物育种, 2022, 20(15):5125-5129.
[13]
沙伟, 李婧, 马天意, 等. 薰衣草及其精油在医疗应用中的研究进展[J/OL]. 分子植物育种:1-8[2021-09-01]. http://kns.cnki.net/kcms/detail/46.1068.S.20210816.1455.008.html.
[14]
BADR M M, BADAWY M E I, TAKTAK N E M. Characterization, antimicrobial activity, and antioxidant activity of the nanoemulsions of Lavandula spica essential oil and its main monoterpenes[J]. Journal of drug delivery science and technology, 2021,65,102732.
[15]
魏婷, 曾永明, 杨国琴, 等. 超声波辅助提取伊犁薰衣草花色苷工艺及其抗氧化活性研究[J]. 食品科技, 2018, 43(12):244-252.
[16]
李楠, 曾永明, 王丽娟, 等. 超声辅助酶法提取薰衣草花色苷及其热降解动力学[J]. 食品工业科技, 2020, 41(1):150-157.
[17]
SIENKIEWICZ M, ŁYSAKOWSKA M, CIEĆWIERZ J, et al. Antibacterial activity of thyme and lavender essential oils[J]. Medicinal chemistry (Shariqah (United Arab Emirates)), 2011, 7(6).
[18]
韩江南. 牡丹花色与花色苷的研究[D]. 洛阳: 河南科技大学, 2010.
[19]
CRUZ A A D L, HILBERT G, RIVIÈREN C, et al. Anthocyanin identification and composition of wild Vitis spp. accessions by using LC-MS and LC-NMR[J]. Analytica chimica acta, 2012, 732:145-152.
[20]
HUANG D, MING R H, TAN Y, et al. Identification of anthocyanins in the fruits of Kadsura coccinea using UPLC-MS/MS-based metabolomics[J]. Biochemical systematics and ecology, 2021, 98:104324.
[21]
樊荣辉, 黄敏玲, 钟淮钦, 等. 鹤望兰黄色花萼色素成分分析[J]. 中国农学通报, 2016, 32(25):65-68.
通过鹤望兰黄色花萼色素成分分析,对研究其花色形成机理具有重要意义。该文对鹤望兰黄色花萼进行紫外-可见光谱分析、花色表型测定和高效液相色谱分析。结果发现,黄色花萼含有类胡萝卜素和黄酮类化合物。在类胡萝卜素中,β-胡萝卜素含量最高,达825.80 μg/(g&middot;FW),其次是β-隐黄质含量[322.28 μg/(g&middot;FW)],含量最少是叶黄素[3.87 μg/(g&middot;FW)]。该项研究为鹤望兰花色素成分的进一步分离和鉴定等工作提供了参考,同时也为鹤望兰花色的分子育种提供帮助。
[22]
高嵩, 李天来. 草原龙胆花瓣花色素苷组成及与其花色的关系[J]. 沈阳农业大学学报, 2020, 51(4):410-416.
[23]
YAMAGISHI M, YOSHIDA Y, NAKAYAMA M. The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of asiatic hybrid lilies (Lilium spp.) and regulates pigment quantity[J]. Molecular breeding, 2012, 30(2):913-925.
[24]
贾新平, 邓衍明, 孙晓波, 等. 二月兰花瓣花色苷组成分析[J]. 中国农学通报, 2018(10):60-64.
为了研究二月兰花色苷成分组成特点,本试验以3份二月兰为试材,利用特征颜色反应确定二月兰花瓣的色素类型,并通过高效液相色谱-质谱联用技术(HPLC-Q-TOF-MS)分析花色苷种类及其含量。研究结果表明,3份二月兰花瓣中均不含类胡萝卜素,除白色花瓣以外,其余均含花色苷。蓝紫色花瓣的花色苷含量最高,粉色次之。在二月兰花瓣中共检测到了7种花色苷成分,通过与已有文献比对,推定其成分为矢车菊素3-芸香糖苷、锦葵素3,5-二葡萄糖苷、矢车菊素3-香豆酰葡萄糖苷-5-葡萄糖苷、矢车菊素3-p-香豆酰二葡萄糖苷-5-葡萄糖苷、矢车菊素3-阿魏酰槐糖苷-5-丙二酰葡萄糖苷、矢车菊素3-香豆酰槐糖苷-5-丙二酰葡萄糖苷、矢车菊素3-(6′-丙二酰葡糖苷-2′-(6′′-香豆酰-2′′-羟基苯甲酰葡糖苷))-5-丙二酰葡糖苷。粉色花瓣中仅含矢车菊素的花色苷衍生物,而蓝紫色花瓣中则含矢车菊素和锦葵素的花色苷衍生物。本研究为二月兰花色苷成分的进一步分离和鉴定工作提供了参考,同时也为二月兰花色的分子育种提供理论依据。
[25]
JIE Z, LIANG S W, JIN M G, et al. Determination of anthocyanins and exploration of relationship between their composition and petal coloration in crape myrtle (Lagerstroemia hybrid)[J]. Journal of integrative plant biology, 2008, 50(5):581-588.
[26]
刘跃平, 周洋丽, 高燕会. 换锦花花色苷成分及其稳定性[J]. 浙江农林大学学报, 2021, 38(3):587-596.

基金

新疆生产建设兵团第四师可克达拉市科技项目“薰衣草花色的形成及相关基因的研究”(2019NY08)
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