抗生素在人工湿地中的去除机制综述

陈高霖, 麦咏芯, 张冬青, 吕梦雨, 李霞

中国农学通报. 2023, 39(9): 63-70

PDF(1230 KB)
PDF(1230 KB)
中国农学通报 ›› 2023, Vol. 39 ›› Issue (9) : 63-70. DOI: 10.11924/j.issn.1000-6850.casb2022-0283
资源·环境·生态·土壤

抗生素在人工湿地中的去除机制综述

作者信息 +

Antibiotic Removal Mechanism in Constructed Wetland: A Review

Author information +
History +

摘要

随着医疗废水和生活污水的排放,抗生素进入水体环境,通过食物链的传递富集累积到人体中,影响人类健康。人工湿地(CWs)是可替代传统污水处理技术的绿色生态系统,然而迄今为止,应用CWs去除抗生素的机制尚未清楚。为了进一步研究抗生素在不同类型CWs中的去除行为,分析了CWs中的基质、微生物和大型植物等关键组成成分,在去除不同抗生素所起到的作用;归纳了去除抗生素的内在关键机制包括土壤基质吸附、植物根系吸收和茎部转移和植物根系微生物降解;总结了影响CWs去除抗生素的因素,包括抗生素的物理化学特性、土壤基质类型、土壤生物酶的种类、pH、氧化还原条件、微生物种类与活性等。最后探讨了利用CWs生态系统提高抗生素去除效率的方法,并就未来减轻CWs中抗生素风险的研究前景进行了简要介绍,旨在为进一步开发可行性、创新性和高效性的抗生素去除技术提供相关信息。

Abstract

With the discharge of medical wastewater and domestic sewage, antibiotics have been frequently detected in the aquatic environment and accumulated in human body through the transmission and accumulation of food chain, posing severe threat to human health. Constructed wetlands (CWs) have been used as green alternatives to conventional wastewater treatment technologies. However, to date, the mechanism of applying CWs to remove antibiotics has not been comprehensively elucidated. In order to clarify the removal behavior of antibiotics in different types of CWs, this study investigates the role of key components, including wetland substrates, microbial organisms and macrophytes, in antibiotic removal in CWs. The key mechanisms involved in antibiotic removal are summarized, including soil substrate adsorption, plant root system uptake and stem translocation, and microbial biodegradation in root system. This study also assesses the factors affecting antibiotic removal in CWs, such as the physiochemical properties of antibiotics, the types of soil substrates, enzyme type, pH, redox conditions, microbial species and activities. In addition, an in-depth discussion about the strategies for enhancing antibiotic removal in CWs ecosystem is carried out, in order to shed a light into further exploration of feasible, innovative, and efficient solutions for antibiotic removal in CWs.

关键词

抗生素 / 人工湿地 / 土壤基质 / 大型植物 / 微生物群落 / 去除机制 / 影响因素

Key words

antibiotic / constructed wetland / soil substrate / macrophytes / microbial community / removal mechanism / influencing factors

引用本文

导出引用
陈高霖 , 麦咏芯 , 张冬青 , 吕梦雨 , 李霞. 抗生素在人工湿地中的去除机制综述. 中国农学通报. 2023, 39(9): 63-70 https://doi.org/10.11924/j.issn.1000-6850.casb2022-0283
CHEN Gaolin , MAI Yongxin , ZHANG Dongqing , LV Mengyu , LI Xia. Antibiotic Removal Mechanism in Constructed Wetland: A Review. Chinese Agricultural Science Bulletin. 2023, 39(9): 63-70 https://doi.org/10.11924/j.issn.1000-6850.casb2022-0283

参考文献

[1]
李新慧, 郑权, 李静, 等. 氟喹诺酮对垂直流人工湿地性能及微生物群落的影响[J]. 环境科学, 2018, 39(10):4809-4816.
[2]
ZHANG Q, YING G, PAN C, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of china: source analysis, multimedia modeling, and linkage to bacterial resistance[J]. Environmental science & technology, 2015, 49(11):6772-6782.
[3]
BINH V N, DANG N, ANH N T K, et al. Antibiotics in the aquatic environment of vietnam: sources, concentrations, risk and control strategy[J]. Chemosphere, 2018, 197:438-450.
The presence of antibiotics in the aquatic environment is a serious concern because it may lead to the emergence of antibiotic resistance, thus lowering the therapeutic effect of antibiotics. In Vietnam, the problem is aggravated by the irrational use of antibiotics in different sectors of agriculture and human health service. Moreover, the residues of antibiotics in the aquatic environment can be spread widely due to the lack of proper wastewater treatment systems. In this paper, we aim to comprehensively review all relevant sources that discharge antibiotics to the aquatic environment in Vietnam. Apart from the common source of antibiotics from aquaculture, other activities that release considerable amounts of antibiotics into water environment are also included. Environmental concentrations of antibiotics related to those sources are studied to demonstrate their contributions to the presence of antibiotics in the aquatic environment in Vietnam. As antibiotic-contained water may be used as water supply for irrigation and even human consumption in rural areas, the essence of wastewater treatment is highlighted. Finally, we also discuss the new National Action plan from the Ministry of Health for controlling the issue of antibiotic resistance in Vietnam.Copyright © 2018 Elsevier Ltd. All rights reserved.
[4]
HASSOUN-KHEIR N, STABHOLZ Y, KREFT J, et al. Comparison of antibiotic-resistant bacteria and antibiotic resistance genes abundance in hospital and community wastewater: a systematic review[J]. Science of the total environment, 2020, 743:140804.
[5]
贾晗, 吴若菁, 黄婧, 等. 生物法处理畜禽养殖污水的研究现状与展望[J]. 水处理技术, 2008, 34(7):7-11.
[6]
DELGADO N, BERMEO L, HOYOS D A, et al. Occurrence and removal of pharmaceutical and personal care products using subsurface horizontal flow constructed wetlands[J]. Water research, 2020, 187:116448.
[7]
HIjOSA-VALSERO M, REYES-CONTRERAS C, DOMINGUEZ C, et al. Behaviour of pharmaceuticals and personal care products in constructed wetland compartments: influent, effluent, pore water, substrate and plant roots[J]. Chemosphere, 2016, 145(FEB):508-517.
[8]
ILYAS H, HULLEBUSCH E D V. Performance comparison of different types of constructed wetlands for the removal of pharmaceuticals and their transformation products: a review[J]. Environmental ence and pollution research, 2020, 27(13):14342-14364.
[9]
SAKURAI K S I, POMPEI C M E, TOMITA I N, et al. Hybrid constructed wetlands as post-treatment of blackwater: an assessment of the removal of antibiotics[J]. Journal of environmental management, 2021, 278:111552.
[10]
ZHONG F, HUANG S, WU J, et al. The use of microalgal biomass as a carbon source for nitrate removal in horizontal subsurface flow constructed wetlands[J]. Ecological Engineering, 2019, 127:263-267.
[11]
SGROI M, PELISSARI C, ROCCARO P, et al. Removal of organic carbon, nitrogen, emerging contaminants and fluorescing organic matter in different constructed wetland configurations[J]. Chemical engineering journal, 2018, 332:619-627.
[12]
LIU M, LI X, HE Y, et al. Aquatic toxicity of heavy metal-containing wastewater effluent treated using vertical flow constructed wetlands[J]. Science of The total environment, 2020, 727:138616.
[13]
ETTEIEB S, ZOLFAGHARI M, MAGDOULI S, et al. Performance of constructed wetland for selenium, nutrient and heavy metals removal from mine effluents[J]. Chemosphere, 2021, 281:130921.
[14]
RAMPRASAD C, PHILIP L. Contributions of various processes to the removal of surfactants and personal care products in constructed wetland[J]. Chemical engineering journal, 2018, 334:322-333.
[15]
PéREZ-LóPEZ M E, ARREOLA-ORTIZ A E, MALAGóN ZAMORA P. Evaluation of detergent removal in artificial wetlands (biofilters)[J]. Ecological Engineering, 2018, 122:135-142.
[16]
TANG X, YANG Y, MCBRIDE M B, et al. Removal of chlorpyrifos in recirculating vertical flow constructed wetlands with five wetland plant species[J]. Chemosphere, 2019, 216:195-202.
[17]
GAULLIER C, BARAN N, DOUSSET S, et al. Wetland hydrodynamics and mitigation of pesticides and their metabolites at pilot-scale[J]. Ecological engineering, 2019, 136:185-192.
[18]
HE Y, ZHANG L, JIANG L, et al. Improving removal of antibiotics in constructed wetland treatment systems based on key design and operational parameters: a review[J]. Journal of hazardous materials, 2020:124386.
[19]
GARCíA J, GARCíA-GALáN M J, DAY J W, et al. A review of emerging organic contaminants (EOCs), antibiotic resistant bacteria (ARB), and antibiotic resistance genes (ARGs) in the environment: increasing removal with wetlands and reducing environmental impacts[J]. Bioresource technology, 2020, 307:123228.
[20]
CHEN M, ZHU M, ZHU Y, et al. Collision of emerging and traditional methods for antibiotics removal: taking constructed wetlands and nanotechnology as an example[J]. Nanoimpact, 2019, 15:100175.
[21]
LIU X, GUO X, LIU Y, et al. A review on removing antibiotics and antibiotic resistance genes from wastewater by constructed wetlands: performance and microbial response[J]. Environmental pollution, 2019, 254:112996.
[22]
ZHANG D, GERSBERG R M, NG W J, et al. Removal of pharmaceuticals and personal care products in aquatic plant-based systems: a review[J]. Environmental pollution, 2014, 184:620-639.
Pharmaceuticals and personal care products (PPCPs) in the aquatic environment are regarded as emerging contaminants and have attracted increasing concern. The use of aquatic plant-based systems such as constructed wetlands (CWs) for treatment of conventional pollutants has been well documented. However, available research studies on aquatic plant-based systems for PPCP removal are still limited. The removal of PPCPs in CWs often involves a diverse and complex set of physical, chemical and biological processes, which can be affected by the design and operational parameters selected for treatment. This review summarizes the PPCP removal performance in different aquatic plant-based systems. We also review the recent progress made towards a better understanding of the various mechanisms and pathways of PPCP attenuation during such phytoremediation. Additionally, the effect of key CW design characteristics and their interaction with the physico-chemical parameters that may influence the removal of PPCPs in functioning aquatic plant-based systems is discussed. Copyright © 2013 Elsevier Ltd. All rights reserved.
[23]
李国婉. 人工湿地基质对磺胺甲恶唑等污染物的同步吸附效果与机制研究[D]. 广州: 华南农业大学, 2018.
[24]
KAH M, SIGMUND G, XIAO F, et al. Sorption of ionizable and ionic organic compounds to biochar, activated carbon and other carbonaceous materials[J]. Water research, 2017, 124:673-692.
The sorption of ionic and ionizable organic compounds (IOCs) (e.g., pharmaceuticals and pesticides) on carbonaceous materials plays an important role in governing the fate, transport and bioavailability of IOCs. The paradigms previously established for the sorption of neutral organic compounds do not always apply to IOCs and the importance of accounting for the particular sorption behavior of IOCs is being increasingly recognized. This review presents the current state of knowledge and summarizes the recent advances on the sorption of IOCs to carbonaceous sorbents. A broad range of sorbents were considered to evaluate the possibility to read across between fields of research that are often considered in isolation (e.g., carbon nanotubes, graphene, biochar, and activated carbon). Mechanisms relevant to IOCs sorption on carbonaceous sorbents are discussed and critically evaluated, with special attention being given to emerging sorption mechanisms including low-barrier, charge-assisted hydrogen bonds and cation-π assisted π-π interactions. The key role played by some environmental factors is also discussed, with a particular focus on pH and ionic strength. Overall the review reveals significant advances in our understanding of the interactions between IOCs and carbonaceous sorbents. In addition, knowledge gaps are identified and priorities for future research are suggested.Copyright © 2017 Elsevier Ltd. All rights reserved.
[25]
王龙, 高旭, 郭劲松, 等. Mg/Al水滑石对水中痕量邻苯二甲酸酯的吸附动力学和热力学[J]. 环境工程学报, 2011, 5(11):2537-2541.
[26]
GOLET E M, XIFRA I, SIEGRIST H, et al. Environmental exposure assessment of fluoroquinolone antibacterial agents from sewage to soil.[J]. Environmental science & technology, 2003, 37(15):3243-3249.
[27]
KLAUS K. Antibiotics in the aquatic environment-a review-part i[J]. Chemosphere, 2009, 75:435-441.
[28]
JEREMY L, CONKLE, CHARISMA L, et al. Competitive sorption and desorption behavior for three fluoroquinolone antibiotics in a wastewater treatment wetland soil[J]. Chemosphere, 2010, 80(11):1353-1359.
Significant amounts of pharmaceuticals are discharged into the environment through wastewater effluent. Sorption has been shown to be a significant aqueous removal pathway for many of these compounds. Competition between ciprofloxacin (CIP), ofloxacin (OFL) and norfloxacin (NOR) and their sorption to, and desorption from, a surrogate Louisiana wastewater treatment wetland soil were investigated to gain insight into the fate and transport of the pollutants within wastewater treatment wetlands. This study was undertaken in the context of a treatment wetland that continuously receives pharmaceuticals. Therefore it is important to understand the total capacity of this soil to sorb these compounds. Sorption to this treatment wetland soil was found to provide a major and potentially long-term removal pathway for these antibiotics from wastewater. LogK(F) values for all three compounds were between 4.09 and 3.90 for sorption and 4.24 and 4.05 microg(1-1/)(n)(cm(3))(1/)(n)g(-1) for desorption. The compounds were sorbed in amounts ranging from 60% to 90% for high and low loading, respectively. The majority of the compounds were sorbed to the soil within the first 20h, indicating that treatment wetland may not need long retention times (weeks to months) in order to remove these compounds. Sorption K(D) values for competition (20 ppm of each compound for 60 ppm of total fluoroquinolones) ranged from 2300 to 3800 cm(3)g(-1) which is between both the 20 (4300-5800 cm(3)g(-1)) and 60 (1300-3000 cm(3)g(-1)) ppm single compound K(D) values, indicating that there is competition between these three compound for sorption sites. Sorption and desorption data (single component and mixture) collectively provide the following evidence: (1) NOR and, to a lesser extent, CIP outcompete OFL for sorption sites, (2) OFL sorbes to its share of "quality" sorption sites, and (3) competition only occurs for lesser "quality" binding sites.Copyright (c) 2010 Elsevier Ltd. All rights reserved.
[29]
严清. 典型PhACs在城市水系统中的迁移分布规律及其在人工湿地中的去除研究[D]. 重庆: 重庆大学, 2014.
[30]
TOLLS J. Sorption of veterinary pharmaceutic. als in soils: a review.[J]. Environmental science & technology, 2001, 35(17):3397-3406.
[31]
MARíA H, GUIDO F, MICHAEL P, et al. Removal of antibiotics from urban wastewater by constructed wetland optimization[J]. Chemosphere, 2011, 83(5):713-719.
[32]
阿丹. 人工湿地对14种常用抗生素的去除效果及影响因素研究[D]. 广州: 暨南大学, 2012.
[33]
CARRASQUILLO A J, BRULAND G L, MACKAY A A, et al. Sorption of ciprofloxacin and oxytetracycline zwitterions to soils and soil minerals: influence of compound structure[J]. Environmental science & technology, 2008, 42(20):7634-7642.
[34]
A D, LI L, TAI Y, et al. Behavior assessment of sulfonamides and N4-acetyl sulfonamides from wastewater effluent in subsurface constructed wetlands: removal, distribution, and biotransformation[J]. Chemical engineering journal, 2020, 396:125252.
[35]
LI Q, LONG Z, WANG H, et al. Functions of constructed wetland animals in water environment protection - a critical review[J]. Science of the total environment, 2021, 760:144038.
[36]
SANTOS F, ALMEIDA C M R D, RIBEIRO I, et al. Removal of veterinary antibiotics in constructed wetland microcosms - response of bacterial communities[J]. Ecotoxicology and environmental safety, 2019, 169:894-901.
This study aimed to evaluate the response of bacteria, in terms of microbial community structure, from constructed wetland (CW) microcosms exposed to two veterinary antibiotics, enrofloxacin (ENR) and ceftiofur (CEF), alone or in a mixture, identifying which bacterial groups were dominant in CWs substrate during livestock wastewater treatment. Wastewater, not-doped or doped with ENR and/or CEF (100 µg/L each), was treated during 18 one-week cycles. Systems showed removal percentages > 85% for the added antibiotics, showing also high removal percentages for nutrients and organic matter and confirming CWs systems were working properly. However, both time of exposure and presence of antibiotics influenced significantly CWs substrate bacterial community structure. Pyrosequencing results showed bacterial communities were dominated by phyla Proteobacteria (38-48%), Firmicutes (20-27%), Bacteroidetes (12-15%) and Actinobacteria (4-9%), and that their relative abundance was clearly affected by the presence of the antibiotics. Results suggest the applicability of CWs for the removal of veterinary antibiotics from livestock wastewaters and provide new knowledge about the bacteria within the system, which can potentially be involved in removal processes. This information could in the future be used to improve CWs removal rates of pharmaceuticals from livestock wastewaters.Copyright © 2018 Elsevier Inc. All rights reserved.
[37]
LI Y, ZHU G, NG W J, et al. A review on removing pharmaceutical contaminants from wastewater by constructed wetlands: design, performance and mechanism[J]. Science of the total environment, 2014, 468-469:908-932.
[38]
杜刚, 黄磊, 高旭, 等. 工湿地中微生物数量与污染物去除的关系[J]. 湿地科学, 2013, 11(1):13-20.
[39]
陈军. 生活污水中抗生素和耐药基因的人工湿地去除机制与系统优化[D]. 广州: 中国科学院大学(中国科学院广州地球化学研究所), 2017.
[40]
DU L, ZHAO Y, WANG C, et al. Removal performance of antibiotics and antibiotic resistance genes in swine wastewater by integrated vertical-flow constructed wetlands with zeolite substrate[J]. Science of the total environment, 2020, 721:137765.
[41]
COSTA F, LAGO A, ROCHA V, et al. A review on biological processes for pharmaceuticals wastes abatement-a growing threat to modern society[J]. Environmental science & technology, 2019, 53(13):7185-7202.
[42]
LENG L, WEI L, XIONG Q, et al. Use of microalgae based technology for the removal of antibiotics from wastewater: a review[J]. Chemosphere, 2020, 238:124680.
[43]
SUTHERLAND D L, RALPH P J. Microalgal bioremediation of emerging contaminants - opportunities and challenges[J]. Water research, 2019, 164:114921.
[44]
SITHOLE B B, GUY R D. Models for tetracycline in aquatic environments[J]. Water, air, and soil pollution, 1987, 32(3-4):315-321.
[45]
POULIQUEN H, LE, BRIS H. Sorption of oxolinic acid and oxytetracycline to marine sediments[J]. Chemosphere, 1996, 33(5):801-815.
[46]
HALLING-SøRENSEN B, NORS NIELSEN S, LANZKY P F, et al. Occurrence, fate and effects of pharmaceutical substances in the environment- a review[J]. Chemosphere, 1998, 36(2):357-393.
[47]
RICHTER R, KAMAL M A M, GARCíA-RIVERA M A, et al. A hydrogel-based in vitro assay for the fast prediction of antibiotic accumulation in gram-negative bacteria[J]. Materials today bio, 2020, 8:100084.
[48]
HANCOCK R E W, BELL A. Antibiotic uptake into gram-negative bacteria[J]. Eur j clin microbiol infect dis, 1988, 7(6):713-720.
[49]
BEDARD J, WONG S, BRYAN L E. Accumulation of enoxacin by escherichia coli and Bacillus subtilis[J]. Antimicrobial agents and chemotherapy, 1987, 31(9):1348-1354.
Several methods were used to determine enoxacin uptake in Escherichia coli strains because washing of cells removed all or most cell-associated enoxacin whereas no washing was associated with large amounts of cell-bound enoxacin. Washing after up to 40 to 45 min of exposure to enoxacin followed by suspension in drug-free medium prevented a significant effect of enoxacin on cell growth. Cell uptakes obtained with different methods showed no difference in the shape of the timed uptake curves but did show significant quantitative differences. These results are consistent with cell-associated enoxacin comprising a freely exchangeable pool of drug. Lineweaver-Burk plots of uptake were consistent with uptake of enoxacin by simple diffusion. No saturability and no competition with ciprofloxacin were observed. Low temperature (4 degrees C) was associated with decreased uptake. Arsenate, carbonyl cyanide m-chlorophenylhydrazone, sodium fluoride, sodium azide, and 2,4-dinitrophenol had no effect on uptake. We conclude that the mechanism of transport of enoxacin into cells is by simple diffusion. Mutants of E. coli with deficiency of outer membrane proteins F and C and an enoxacin-resistant mutant selected by serial passage with increasing enoxacin concentrations demonstrated that F porins play a significant role in enoxacin uptake and influence susceptibility to enoxacin. Uptake was shown to be similar in a strain of Bacillus subtilis.
[50]
XIAO R, ZHENG Y. Overview of microalgal extracellular polymeric substances (EPS) and their applications[J]. Biotechnology advances, 2016, 34(7):1225-1244.
Microalgae have been studied as natural resources for a number of applications, most particularly food, animal feed, biofuels, pharmaceuticals, and nutraceuticals. In addition to the intracellular compounds of interest, microalgae can also excrete various extracellular polymeric substances (EPS) into their immediate living environment during their life cycle to form a hydrated biofilm matrix. These microalgal EPS mainly consist of polysaccharides, proteins, nucleic acids and lipids. Most notably, EPS retain their stable matrix structure and form a 3-D polymer network for cells to interact with each other, and mediate their adhesion to surfaces. EPS also play a role as extracellular energy and carbon sinks. They are also abundant source of structurally and compositionally diverse biopolymers which possess unique bioactivities for special high-value applications, specifically as antivirals, antitumor agents, antioxidants, anticoagulants and anti-inflammatories. Their superior rheological properties also make microalgal EPS particularly useful in mechanical engineering (e.g., biolubricants and drag reducers) and food science/engineering (e.g., thickener and preservatives) applications. The chemical composition and structure of EPS appear to correlate with their applications, but the fundamentals of such relationship are not well understood. This article summarizes previous research on microalgal EPS derived from green algae, diatoms and red algae, including compositions/functions/structure, production, and potential applications. The importance of exopolysaccharides and EPS proteins, with their particular metabolic characteristics, are also described because of their potential high-value applications. This review concludes with potential future research areas of microalgal EPS.Copyright © 2016 Elsevier Inc. All rights reserved.
[51]
YU H. Molecular insights into extracellular polymeric substances in activated sludge[J]. Environmental science & technology, 2020, 54(13):7742-7750.
[52]
XU Q, HAN B, WANG H, et al. Effect of extracellular polymer substances on the tetracycline removal during coagulation process[J]. Bioresource technology, 2020, 309:123316.
[53]
LIU H, HU Z, JIANG L, et al. Roles of carbon source-derived extracellular polymeric substances in solids accumulation and nutrient removal in horizontal subsurface flow constructed wetlands[J]. Chemical engineering journal, 2019, 362:702-711.
[54]
LIGI T, OOPKAUP K, TRUU M, et al. Characterization of bacterial communities in soil and sediment of a created riverine wetland complex using high-throughput 16S rRNA amplicon sequencing[J]. Ecological engineering, 2014, 72:56-66.
[55]
ADRADOS B, SáNCHEZ O, ARIAS C A, et al. Microbial communities from different types of natural wastewater treatment systems: vertical and horizontal flow constructed wetlands and biofilters[J]. Water research, 2014, 55:304-312.
The prokaryotic microbial communities (Bacteria and Archaea) of three different systems operating in Denmark for the treatment of domestic wastewater (horizontal flow constructed wetlands (HFCW), vertical flow constructed wetlands (VFCW) and biofilters (BF)) was analysed using endpoint PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE). Further sequencing of the most representative bacterial bands revealed that diverse and distinct bacterial communities were found in each system unit, being γ-Proteobacteria and Bacteroidetes present mainly in all of them, while Firmicutes was observed in HFCW and BF. Members of the Actinobacteria group, although found in HFCW and VFCW, seemed to be more abundant in BF units. Finally, some representatives of α, β and δ-Proteobacteria, Acidobacteria and Chloroflexi were also retrieved from some samples. On the other hand, a lower archaeal diversity was found in comparison with the bacterial population. Cluster analysis of the DGGE bacterial band patterns showed that community structure was related to the design of the treatment system and the organic matter load, while no clear relation was established between the microbial assemblage and the wastewater influent. Copyright © 2014 Elsevier Ltd. All rights reserved.
[56]
ZHOU X, CHEN Z, LI Z, et al. Impacts of aeration and biochar addition on extracellular polymeric substances and microbial communities in constructed wetlands for low C/N wastewater treatment: implications for clogging[J]. Chemical engineering journal, 2020, 396:125349.
[57]
严佳丽, 陈湖星, 杨杨, 等. 一株高效DEHP降解菌的分离、鉴定及其降解特性[J]. 微生物学通报, 2014, 41(8):1532-1540.
[58]
WANG J, WANG S. Microbial degradation of sulfamethoxazole in the environment[J]. Applied microbiology and biotechnology, 2018, 102(8):3573-3582.
Sulfamethoxazole (SMX) is one of the most widely applied sulfonamide antibiotics in the world, which is becoming a ubiquitous pollutant in the environment. In this mini-review, the microbial degradation of SMX was briefly reviewed. The performance of the conventional wastewater treatment plants in removing SMX was provided. The microorganisms capable of degrading SMX, including mixed cultures and pure cultures, were presented. The effects of environmental conditions such as temperature, pH, initial SMX concentration, and additional carbon sources on the biodegradation of SMX were discussed. The metabolic pathways of SMX degradation were summarized. Finally, the suggestions were made for further studies.
[59]
CHENG D L, NGO H H, GUO W S, et al. Bioprocessing for elimination antibiotics and hormones from swine wastewater[J]. Science of the total environment, 2018, 621:1664-1682
[60]
韩蕊, 王冬莹, 芮洋, 等. 一株降解邻苯二甲酸酯真菌的筛选及其降解特性研究[J]. 环境科学学报, 2013, 33(11):2941-2946.
[61]
刘琳, 王炜, 余健. 曝气生物滤池去除邻苯二甲酸酯的试验研究[J]. 工业水处理, 2012, 32(12):62-65.
[62]
NAVACHAROEN A, VANGNAI A S. Biodegradation of diethyl phthalate by an organic-solvent-tolerant Bacillus subtilis strain 3C3 and effect of phthalate ester coexistence[J]. International biodeterioration & biodegradation, 2011, 65(6):818-826.
[63]
WU X, WANG Y, DAI Q, et al. Isolation and characterization of four di-n-butyl phthalate (DBP)-degrading Gordonia sp. strains and cloning the 3, 4-phthalate dioxygenase gene[J]. World journal of microbiology and biotechnology, 2011, 27(11):2611-2617.
[64]
BOUjU H, RICKEN B, BEFFA T, et al. Isolation of bacterial strains capable of sulfamethoxazole mineralization from an acclimated membrane bioreactor[J]. Applied and environmental microbiology, 2012, 78(1):277-279.
In this study, we isolated five strains capable of degrading ¹⁴C-labeled sulfamethoxazole to ¹⁴CO₂ from a membrane bioreactor acclimatized to sulfamethoxazole, carbamazepine, and diclofenac. Of these strains, two belonged to the phylum Actinobacteria, while three were members of the Proteobacteria.
[65]
LIU L, LI J, XIN Y, et al. Evaluation of wetland substrates for veterinary antibiotics pollution control in lab-scale systems[J]. Environmental pollution, 2021, 269:116152.
[66]
ROGERS H R. Sources, behaviour and fate of organic contaminants during sewage treatment and in sewage sludges[J]. Science of the total environment, 1996, 185(1):23-26.
[67]
XIONG J, KURADE M B, JEON B. Can microalgae remove pharmaceutical contaminants from water?[J]. Trends in biotechnology, 2018, 36(1):30-44.
[68]
LIU Y, LIU X, LU S, et al. Adsorption and biodegradation of sulfamethoxazole and ofloxacin on zeolite: influence of particle diameter and redox potential[J]. Chemical engineering journal, 2020, 384:123346.
[69]
CHEN J, TONG T, JIANG X, et al. Biodegradation of sulfonamides in both oxic and anoxic zones of vertical flow constructed wetland and the potential degraders[J]. Environmental pollution, 2020, 265:115040.
[70]
MENG P, PEI H, HU W, et al. How to increase microbial degradation in constructed wetlands: influencing factors and improvement measures[J]. Bioresource technology, 2014, 157:316-326.
Microorganisms play a vital role in degradation of multiple pollutants in constructed wetlands (CWs). Thus, the search for methods to improve microbial degradation in CWs is crucial. This study provides a review of critical parameters including availability of organic carbon, redox condition, temperature, pH, presence of plants, media characteristics and their influences on microbial processes. Current strategies focusing on regulation of carbon source, redox condition, and choice of substrates to enhance microbial activity in CWs are also described. A special emphasis is given to the application of bioaugmentation to enhance microbial activities in wetland in future research. Copyright © 2014 Elsevier Ltd. All rights reserved.
[71]
REIS P J M, HOMEM V, ALVES A, et al. Insights on sulfamethoxazole bio-transformation by environmental proteobacteria isolates[J]. Journal of hazardous materials, 2018, 358:310-318.
Although sulfonamide residues are frequently reported as freshwaters contaminants, information on the ability of native bacteria to modify these synthetic antibiotics is scarce. Our purpose was to investigate the potential of bacteria from different aquatic environments to cleave or transform sulfamethoxazole (SMX) and infer on their ability to reduce the toxicity of this antibiotic. From a collection of about 100 Proteobacteria, 47 strains previously isolated from drinking water, surface water, and wastewater grew in the presence of 200 μM, and were further studied. Out of these, 14 strains, mostly from mineral drinking water, transformed SMX into equimolar amounts of the lesser toxic derivative N-acetyl-sulfamethoxazole. The highest percentage of SMX transformation was recorded for two strains affiliated to Pseudomonas mandelii. For P. mandelii McBPA4 higher SMX transformation rate and extent were observed in fed-batch (∼8 μM/h, 81%) than in batch conditions (∼5 μM/h, 25%), but similar specific transformation rates were found in both cultivation modes (∼20 μmol/g/h), indicating the dependence of the process on the microbial load. These results evidence that the capacity to transform synthetic antibiotics may be common among bacteria and highlight the potential of environmental bacteria in attenuating the potential adverse effects of pollution with sulfonamides.Copyright © 2018 Elsevier B.V. All rights reserved.
[72]
RICKEN B, KOLVENBACH B A, BERGESCH C, et al. FMNH(2)-dependent monooxygenases initiate catabolism of sulfonamides in Microbacterium sp. strain BR1 subsisting on sulfonamide antibiotics[J]. Scientific reports, 2017, 7(1):15783.
We report a cluster of genes encoding two monooxygenases (SadA and SadB) and one FMN reductase (SadC) that enable Microbacterium sp. strain BR1 and other Actinomycetes to inactivate sulfonamide antibiotics. Our results show that SadA and SadC are responsible for the initial attack of sulfonamide molecules resulting in the release of 4-aminophenol. The latter is further transformed into 1,2,4-trihydroxybenzene by SadB and SadC prior to mineralization and concomitant production of biomass. As the degradation products lack antibiotic activity, the presence of SadA will result in an alleviated bacteriostatic effect of sulfonamides. In addition to the relief from antibiotic stress this bacterium gains access to an additional carbon source when this gene cluster is expressed. As degradation of sulfonamides was also observed when Microbacterium sp. strain BR1 was grown on artificial urine medium, colonization with such strains may impede common sulfonamide treatment during co-infections with pathogens of the urinary tract. This case of biodegradation exemplifies the evolving catabolic capacity of bacteria, given that sulfonamide bacteriostatic are purely of synthetic origin. The wide distribution of this cluster in Actinomycetes and the presence of traA encoding a relaxase in its vicinity suggest that this cluster is mobile and that is rather alarming.
[73]
SHAO S, HU Y, CHENG J, et al. Biodegradation mechanism of tetracycline (TEC) by strain klebsiellasp. SQY5 as revealed through products analysis and genomics[J]. Ecotoxicology and environmental safety, 2019, 185:109676.
[74]
BILAL M, ADEEL M, RASHEED T, et al. Emerging contaminants of high concern and their enzyme-assisted biodegradation-a review[J]. Environment international, 2019, 124:336-353.
[75]
WANG C, ZHENG S, WANG P, et al. Effects of vegetations on the removal of contaminants in aquatic environments: a review[J]. Journal of hydrodynamics, ser. b, 2014, 26(4):497-511.
[76]
DAN A, YANG Y, DAI Y, et al. Removal and factors influencing removal of sulfonamides and trimethoprim from domestic sewage in constructed wetlands[J]. Bioresource technology, 2013, 146:363-370.
Twelve pilot-scale constructed wetlands with different configurations were set up in the field to evaluate the removal and factors that influence removal of sulfonamides (sulfadiazine, sulfapyridine, sulfacetamide, sulfamethazine and sulfamethoxazole) and trimethoprim from domestic sewage. The treatments included four flow types, three substrates, two plants and three hydraulic loading rates across two seasons (summer and winter). Most target antibiotics were efficiently removed by specific constructed wetlands; in particular, all types of constructed wetlands performed well for the degradation of sulfapyridine. Flow types were the most important influencing factor in this study, and the best removal of sulfonamides was achieved in vertical subsurface-flow constructed wetlands; however, the opposite phenomenon was found with trimethoprim. Significant relationships were observed between antibiotic degradation and higher temperature and redox potential, which indicated that microbiological pathways were the most probable degradation route for sulfonamides and trimethoprim in constructed wetlands. Copyright © 2013 Elsevier Ltd. All rights reserved.
[77]
HUANG X, YE G, YI N, et al. Effect of plant physiological characteristics on the removal of conventional and emerging pollutants from aquaculture wastewater by constructed wetlands[J]. Ecological engineering, 2019, 135:45-53.
[78]
MAN Y, WANG J, TAM N F, et al. Responses of rhizosphere and bulk substrate microbiome to wastewater-borne sulfonamides in constructed wetlands with different plant species[J]. Science of the total environment, 2020, 706:135955.
[79]
李琴. 会仙湿地植物根际对磺胺类抗生素降解的影响机理研究[D]. 桂林: 桂林理工大学, 2021.
[80]
程宪伟, 梁银秀, 祝惠, 等. 工湿地处理水体中抗生素的研究进展[J]. 湿地科学, 2017, 15(1):125-131.
[81]
荣婧. 水生植物对水中磺胺嘧啶和左炔诺孕酮去除机理研究[D]. 重庆: 重庆大学, 2011.
[82]
HU H, ZHOU Q, LI X, et al. Phytoremediation of anaerobically digested swine wastewater contaminated by oxytetracycline via Lemna aequinoctialis : nutrient removal, growth characteristics and degradation pathways[J]. Bioresource technology, 2019, 291(C):121853.
[83]
周海东, 黄丽萍, 陈晓萌, 等. 人工生态系统对城市河流中抗生素和ARGs的去除[J]. 环境科学, 2021, 42(2):850-859.
[84]
ZHAO M L, ZHAO J, YUAN J, et al. Root exudates drive soil-microbe-nutrient feedbacks in response to plant growth[J]. Plant, cell & environment, 2020, 44(2):613-628.
[85]
CHUNG H S, LEE Y J, RAHMAN M, et al. Uptake of the veterinary antibiotics chlortetracycline, enrofloxacin, and sulphathiazole from soil by radish[J]. Science of the total environment, 2017,605-606:322-331.
[86]
ZHANG Y P, SALLACH J B, LAURIE H, et al. Effects of soil texture and drought stress on the uptake of antibiotics and the internalization of salmonella in lettuce following wastewater irrigation[J]. Environmental pollution, 2016, 208(Pt B):523-531.
Treated wastewater is expected to be increasingly used as an alternative source of irrigation water in areas facing fresh water scarcity. Understanding the behaviors of contaminants from wastewater in soil and plants following irrigation is critical to assess and manage the risks associated with wastewater irrigation. The objective of this study was to evaluate the effects of soil texture and drought stress on the uptake of antibiotics and the internalization of human pathogens into lettuce through root uptake following wastewater irrigation. Lettuce grown in three soils with variability in soil texture (loam, sandy loam, and sand) and under different levels of water stress (no drought control, mild drought, and severe drought) were irrigated with synthetic wastewater containing three antibiotics (sulfamethoxazole, lincomycin and oxytetracycline) and one Salmonella strain a single time prior to harvest. Antibiotic uptake in lettuce was compound-specific and generally low. Only sulfamethoxazole was detected in lettuce with increasing uptake corresponding to increasing sand content in soil. Increased drought stress resulted in increased uptake of lincomycin and decreased uptake of oxytetracycline and sulfamethoxazole. The internalization of Salmonella was highly dependent on the concentration of the pathogen in irrigation water. Irrigation water containing 5 Log CFU/mL Salmonella resulted in limited incidence of internalization. When irrigation water contained 8 Log CFU/mL Salmonella, the internalization frequency was significantly higher in lettuce grown in sand than in loam (p = 0.009), and was significantly higher in lettuce exposed to severe drought than in unstressed lettuce (p = 0.049). This work demonstrated how environmental factors affected the risk of contaminant uptake by food crops following wastewater irrigation.Copyright © 2015 Elsevier Ltd. All rights reserved.
[87]
黄丹, 叶茂, 朱国繁, 等. 抗生素/抗性细菌/抗性基因在土壤-植物系统中迁移转化及阻控消减的研究进展[J]. 土壤, 2020, 52(5):891-900.
[88]
KONG W D, ZHU Y G, LIANG Y C, et al. Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.).[J]. Environmental pollution (Barking, Essex:1987), 2007, 147(1):187-193.
[89]
FRANKLIN A M, WILLIAMS C F, ANDREWS D M, et al. Uptake of three antibiotics and an antiepileptic drug by wheat crops spray irrigated with wastewater treatment plant effluent[J]. Journal of environmental quality, 2016, 45(2):546.
With rising demands on water supplies necessitating water reuse, wastewater treatment plant (WWTP) effluent is often used to irrigate agricultural lands. Emerging contaminants, like pharmaceuticals and personal care products (PPCPs), are frequently found in effluent due to limited removal during WWTP processes. Concern has arisen about the environmental fate of PPCPs, especially regarding plant uptake. The aim of this study was to analyze uptake of sulfamethoxazole, trimethoprim, ofloxacin, and carbamazepine in wheat ( L.) plants that were spray-irrigated with WWTP effluent. Wheat was collected before and during harvest, and plants were divided into grain and straw. Subsamples were rinsed with methanol to remove compounds adhering to surfaces. All plant tissues underwent liquid-solid extraction, solid-phase extraction cleanup, and liquid chromatography-tandem mass spectrometry analysis. Residues of each compound were present on most plant surfaces. Ofloxacin was found throughout the plant, with higher concentrations in the straw (10.2 ± 7.05 ng g) and lower concentrations in the grain (2.28 ± 0.89 ng g). Trimethoprim was found only on grain or straw surfaces, whereas carbamazepine and sulfamethoxazole were concentrated within the grain (1.88 ± 2.11 and 0.64 ± 0.37 ng g, respectively). These findings demonstrate that PPCPs can be taken up into wheat plants and adhere to plant surfaces when WWTP effluent is spray-irrigated. The presence of PPCPs within and on the surfaces of plants used as food sources raises the question of potential health risks for humans and animals. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.
[90]
AN J, CHEN H W, WEI S H, et al. Antibiotic contamination in animal manure, soil, and sewage sludge in Shenyang, northeast China[J]. Environmental earth sciences, 2015, 74(6):5077-5086.
[91]
HUANG Y J, CHENG M M, LI W H. Simultaneous extraction of four classes of antibiotics in soil, manure and sewage sludge and analysis by liquid chromatography-tandem mass spectrometry with the isotope-labelled internal standard method[J]. Analytical methods, 2013, 5(15):3721-3731.
[92]
QIAO M, CHEN W D, SU J Q, et al. Fate of tetracyclines in swine manure of three selected swine farms in China[J]. Journal of environmental sciences, 2012, 24(6):1047-1052.
[93]
孔维栋, 朱永官. 抗生素类兽药对植物和土壤微生物的生态毒理学效应研究进展[J]. 生态毒理学报, 2007(1):1-9.
[94]
李兆君, 姚志鹏, 张杰, 等. 兽用抗生素在土壤环境中的行为及其生态毒理效应研究进展[J]. 生态毒理学报, 2008(1):15-20.
[95]
LIU F, YING G G, TAO R, et al. Effects of six selected antibiotics on plant growth and soil microbial and enzymatic activities[J]. Environmental pollution, 2008, 157(5):1636-1642.
[96]
LIU L, LIU Y H, LIU C X, et al. Potential effect and accumulation of veterinary antibiotics in phragmites australis under hydroponic conditions[J]. Ecological engineering, 2013, 53:138-143.
[97]
李丽, 杨扬, 陶然, 等. 垂直流-水平潜流组合湿地对磺胺类抗生素的去除[J]. 安全与环境学报, 2014, 14(3):233-239.
[98]
廖德润, 刘超翔, 王振, 等. 兽用抗生素胁迫对空心菜的影响研究[J]. 环境科学学报, 2013, 33(9):2558-2564.
[99]
郑茂佳, 张恩栋, 孙静茹, 等. 四环素类抗生素生物降解研究进展[J]. 天津农业科学, 2018, 24(6):72-76,85.
[100]
MILLER E L, NASON S L, KARTHIKEYAN K G, et al. Root uptake of pharmaceuticals and personal care product ingredients[J]. Environmental science & technology, 2016, 50(2):525-541.
[101]
CARVALHO P N, BASTO M C P, ALMEIDA C M R, et al. A review of plant-pharmaceutical interactions: from uptake and effects in crop plants to phytoremediation in constructed wetlands[J]. Environmental science and pollution research, 2014, 21(20):11729-11763.
[102]
RAI P K, KIM K, LEE S S, et al. Molecular mechanisms in phytoremediation of environmental contaminants and prospects of engineered transgenic plants/microbes[J]. Science of the total environment, 2020, 705:135858.
[103]
TAI Y, TAM F Y, RUAN W, et al. Specific metabolism related to sulfonamide tolerance and uptake in wetland plants[J]. Chemosphere, 2019, 227:496-504.
Wetland plants are proven to perform well in water treatment. However, the phytoremediation capability of wetland plants for antibiotics, especially the uptake and metabolism involved in vivo, is poorly understood. In this study, we investigated the removal, uptake, and specific metabolism by Canna indica and Iris pseudacorus of five sulfonamides (SAs) using hydroponic experiments for seven days. The removal of SAs ranged from 15.2% to 98.4% in the planted groups, whereas that in the unplanted control group was much lower (12.6%-39.9%). The accumulation of SAs in plants was in a concentration-dependent manner via an active process and is not a major removal mechanism (constituted 0.31%-3.62% of the total removal load in plant system). The results also showed differences in the removal and accumulation by plant species of SAs. The acetyl conjugates (N-acetyl SA) were formed, which significantly enhanced the uptake of SAs (P < 0.001) except sulfapyridine. The concentrations of N-acetyl SA accounted for only 0.4%-23.8% of the total SAs distribution in plants, suggesting the involvement of other metabolism pathways. Methylation and oxidation metabolites were identified in plant tissues and no SA-induced growth stress occurred, revealing that antibiotic metabolism in vivo should be associated with the ability of wetland plants to accumulate antibiotic and tolerate antibiotic stress.Copyright © 2019 Elsevier Ltd. All rights reserved.
[104]
ZHANG D Q, GERSBERG R M, NG W J, et al. Removal of pharmaceuticals and personal care products in aquatic plant-based systems: a review[J]. Environmental pollution, 2014, 184:620-639.
Pharmaceuticals and personal care products (PPCPs) in the aquatic environment are regarded as emerging contaminants and have attracted increasing concern. The use of aquatic plant-based systems such as constructed wetlands (CWs) for treatment of conventional pollutants has been well documented. However, available research studies on aquatic plant-based systems for PPCP removal are still limited. The removal of PPCPs in CWs often involves a diverse and complex set of physical, chemical and biological processes, which can be affected by the design and operational parameters selected for treatment. This review summarizes the PPCP removal performance in different aquatic plant-based systems. We also review the recent progress made towards a better understanding of the various mechanisms and pathways of PPCP attenuation during such phytoremediation. Additionally, the effect of key CW design characteristics and their interaction with the physico-chemical parameters that may influence the removal of PPCPs in functioning aquatic plant-based systems is discussed. Copyright © 2013 Elsevier Ltd. All rights reserved.

基金

大学生创新创业训练计划“药物化合物在人工湿地中去除的分子机制研究”(73321108)
PDF(1230 KB)

Accesses

Citation

Detail

段落导航
相关文章

/