
干旱胁迫对不同年代谷子苗期根系表型的影响
Effects of Drought Stress on Root Phenotype of Foxtail Millet Seedlings of Varieties of Different Decades
作为种植于中国西北干旱和半干旱区域重要的粮食作物,谷子具有较高的营养价值。但近年来干旱频发,导致谷子品种退化、品质下降。因根系是运输有机质的重要器官,对植物生长发育的全过程至关重要,选取3个不同年代的6个谷子品种为研究对象,采用土培法人工模拟干旱环境(出苗10天后一组停止浇水至收获;另一组正常浇水,70%±5% FWC),探究品种更替过程中干旱胁迫对谷子苗期根系表型的影响机理。结果表明,不同年代谷子幼苗根系对干旱胁迫的响应机制存在差异,随年代推进,谷子根系干质量、根长、根表面积和根体积呈先降后升趋势。自20世纪70年代以来,1970s—1980s和2000s—2010s谷子在干旱环境中的生长速率分别显著提升了18.1%和30.1%(P<0.05),根冠比有降低趋势并稳定在0.21左右,根条数稳定在9~10条。干旱胁迫下,1960s—1970s谷子的生物量总干重、根宽度、根总长、根表面积和根体积分别显著降低37.6%、47.9%、36.8%、40.1%和30.6%(P<0.05),根密度无显著差异(P>0.05);1970s—1980s谷子的总干重、根宽度和根条数分别显著增加59.8%、21.4%和27%(P<0.05),根表面积、根体积和根总长没有显著差异(P>0.05);2000s—2010s谷子的根宽度、根总长和根长比分别显著降低28.5%、15.3%和35.9%(P<0.05),根表面积、根体积和比根长无显著差异(P>0.05)。本研究结果表明,干旱胁迫下,现代品种苗期根系的敏感度降低,表型差异缩小,耐性提高。
Millet is an important food crop grown in arid and semi-arid regions of northwest China and has high nutritional value. However, in recent years, frequent droughts have led to the degradation of millet varieties and the decline of quality. Because the root system is an important organ to transport organic matter and is critical to the whole process of plant growth and development, six varieties of millet of three different decades were selected as the research objects, and the soil culture method was used to simulate the drought environment (10 days after emergence, one group stopped watering until harvest, another group was irrigated normally, 70%±5% FWC) to explore the mechanism of drought stress on millet seedling root phenotype in the process of variety replacement. The results showed that the response mechanism of millet seedling roots to drought stress was different in varieties of different decades. With the advance of the variety replacement, the root dry mass, root length, root surface area and root volume of millet seedlings decreased first and then increased. The growth rate of millet in drought environment increased by 18.1% and 30.1% in 1970s—1980s varieties and 2000s—2010s varieties, respectively (P<0.05). The root-shoot ratio tended to decrease and stabilized at about 0.21, and the number of roots stabilized at 9-10. Under drought stress, the total dry biomass, root width, total root length, root surface area and root volume of the 1960s—1970s varieties decreased significantly by 37.6%, 47.9%, 36.8%, 40.1% and 30.6% (P<0.05) respectively, while the root density had no significant difference (P>0.05). For varieties of 1970s—1980s, the total dry mass, root width and root number increased by 59.8%, 21.4% and 27% (P<0.05), respectively, while the root surface area, root volume and total root length had no significant difference (P>0.05). For varieties of 2000s—2010s, the root width, total root length and ratio of root length decreased significantly by 28.5%, 15.3% and 35.9% (P<0.05), while the root surface area, root volume and specific root length had no significant difference (P>0.05). The results of this study showed that under drought stress, the root sensitivity of modern millet varieties at seedling stage decreased, the phenotypic difference was narrowed, and the tolerance was improved.
谷子 / 干旱胁迫 / 品种更替 / 根系表型 {{custom_keyword}} /
millet / drought stress, variety replacement / root phenotype {{custom_keyword}} /
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\n\nFoxtail millet (Setaria italica (L.) P.Beauv.) is an ancient cereal cultivated worldwide in arid and marginal lands. It is an ideal crop for the changing climate, with high photosynthetic efficiency. A trait-based selection for drought tolerance is sought for yield stability. The present work had segregated the drought yield as total water use (T), transpiration efficiency (TE) and harvest index (HI) and assessed the importance of these components and their association with drought tolerance. The core collection of foxtail millet germplasm (n = 155) was evaluated in mini-lysimeters under both terminal drought stress (DS) and well-watered (WW) environments. The contribution of T to grain yield under drought was minor but the contribution of TE was positive and of HI negative. Crop duration, T and TE positively influenced, and HI negatively influenced, shoot biomass production. Under drought, the core germplasm accessions varied in shoot biomass, grain yield, HI and T by >3-fold and in TE by 2-fold. Categorisation of the germplasm for TE had differentiated groups of accessions as high TE (n = 17) and low TE (n = 22). Among the three races of foxtail millet, indica was strong for T and TE, and maxima and moharia for HI, with useful exceptions.\n
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旨在为旱区谷子生产及抗旱育种提供理论依据。以10 个谷子品种(系)为研究对象,采用管栽试验,研究了干旱胁迫对不同基因型谷子生长及产量相关性状的影响。结果表明,在干旱胁迫条件下,参试的10 个谷子品种(系),叶绿素含量下降的幅度为4.90%~33.11%,株高降低的幅度为9.56%~30.93%,穗长减小的幅度为5.58%~30.90%,穗粗减小的幅度为3.42%~16.34%,单穗重降低的幅度为7.30%~45.71%,单穗粒重降低的幅度为11.83%~54.72%,出谷率降低的幅度为4.89%~16.60%。抗旱性评价结果表明,‘冀谷31’、‘沧谷5号’、‘冀谷19’等3个品种的抗旱性较好。
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为了在现有谷子品种的基础上,明确有利于提高谷子籽粒营养品质和食味品质的生长环境,本文对‘长农38号’、‘长农39号’、‘长农41号’谷子在长治和沁县两个生态点谷子籽粒的营养品质与食味品质进行比较分析。结果表明:种植地区对‘长农38号’谷子籽粒的维生素B1、直链淀粉、粗蛋白和‘长农41号’的维生素B1、粗脂肪的影响较大,变异系数均在10%以上;对‘长农39号’谷子籽粒的营养品质影响较小,变异系数均在10%以下。通过食味品质的比较可以看出种植在沁县的‘长农38号’谷子籽粒的一致性高于长治,种植在长治的‘长农39号’、‘长农41号’谷子籽粒的一致性低于沁县;种植于长治的‘长农38号’、‘长农39号’谷子籽粒的色泽高于沁县,种植于长治的‘长农41号’谷子籽粒色泽与沁县的评分结果一样;种植于沁县的3个基因型谷子籽粒食味品质的分数合计结果均高于长治。因此通过优化谷子栽培管理措施对提高谷子籽粒品质具有重要意义。
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【目的】 分析新疆早熟陆地棉品种更替产量提高过程中株型特征及主要经济性状的演变趋势,结合发展机采棉对品种特性的需求,阐述品种更替中适宜机采特性的变化,为新疆棉花新品种选育及栽培管理提供理论依据。【方法】 于2015—2016年和2018年选择新疆近40年来自育早熟陆地棉(Gossypium hirsutum L.)不同年代(1980s、1990s、2000s和2010s)大面积主栽品种(新陆早1号、新陆早7号、新陆早13号和新陆早45号)为材料,在膜下滴灌栽培条件下,对不同年代品种的第一果节长度、果枝节间长度、节枝比、株高、果枝始节、始节高度、果枝夹角、果枝数、叶枝数、倒四叶宽和茎粗共11个株型指标及主要经济性状的演变进行分析。【结果】 随品种更替,棉株第一果节长度、果枝节间长度和节枝比逐渐增加,株型由紧凑型向较松散型转变;株高、果枝始节和始节高度逐渐增加,上部果枝与主茎的夹角逐渐减小,果枝上举,具有高产株型特征;根据棉花机采对品种特性的要求,2010s品种果枝始节高度和果枝角度较符合机采棉对株型的要求;不同年代品种间果枝数、叶枝数、倒四叶宽和茎粗无明显差异。皮棉产量、总铃数和衣分均随品种更替逐渐增加,其中1980s、1990s、2000s和2010s品种皮棉产量较当年区域试验产量分别高23%—53%、16%—20%、13%—14%和-2%—6%,膜下滴灌现代高产栽培技术对产量的提高有重要作用,但2000s和2010s品种收获指数显著低于1990s品种。与1980s和1990s品种相比,2010s品种上部铃期短4—5 d,吐絮相对集中,对脱叶剂敏感,吐絮率均在95%以上,无显著差异,但生育期偏长;与审定时品种的生育期相比,1980s和1990s品种提前了3—7 d,2000s和2010s品种提前了0—3 d,这可能与膜下滴灌促早熟栽培技术应用有关。2000s和2010s品种棉纤维长度、比强度、伸长率和纺纱一致性明显改善,但马克隆值相对偏大,纤维强度的改善是以牺牲纤维细度为代价,纤维品质协调性不佳。【结论】 品种更替产量提高过程中,棉花经济性状改善,但品种株型由紧凑型向较松散型转变,生育期偏长,收获指数偏低,棉纤维马克隆值偏大;随着机采棉种植模式的应用,选育和选用纤维品质优、适宜机采的品种是保障新疆棉花产业稳步发展的关键。
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张宁宁, 严加坤, 王小林, 等. 陕北谷子品种更替过程中产量及农艺性状的演变[J]. 应用生态学报, 2021, 32(4):1337-1344.
选择不同年代(1960s—1970s、1980s—1990s、2000s—2010s)在陕北种植的6个谷子品种为试验材料,于2018—2019年在陕西榆林进行田间试验,研究分析了不同年代品种谷子产量及其农艺性状的演变特征。结果表明: 随年代更替,谷子品种产量呈现明显增加趋势,2000s—2010s选育的品种平均产量分别为0.46(2018)和0.66 kg·m<sup>-2</sup>(2019),较2000s前的品种显著增产,增产幅度22%~53%;株高、穗重、叶重和杆重与产量趋势类似;而千粒重和穗长表现相对稳定,随品种更替并未呈现明显变化;旗叶宽度虽然变幅不大,但随品种更替呈明显增大趋势。相关性分析表明,穗重、杆重、叶重和株高与产量之间呈极显著相关。主成分分析显示,2000s—2010s谷子品种具有更大的优势。陕北谷子品种更替过程中主要通过株高和穗重的改良来增加单位面积产量,未来谷子高产育种应注重株高、穗重、杆重和叶重性状改良,尤其应探索适合机械收割的最优株高。
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王振华, 刘鑫, 余爱丽, 等. 不同谷子品种萌发期对干旱胁迫生理响应的变化及抗旱指标筛选[J]. 中国农业科技导报, 2020, 22(12):39-49.
为了探讨干旱胁迫对谷子萌芽期的影响,筛选抗旱谷子新品种及谷子萌芽期抗旱性相关指标,明确干旱胁迫对不同谷子品种萌发期生理特征变化影响,以20%的聚乙二醇(PEG-6000)模拟干旱胁迫对15个谷子品种进行了萌发期抗旱性鉴定,对相关的六个抗旱性指标进行了筛选和相关性研究,并通过测定15个品种在干旱胁迫条件下SOD、POD和CAT的活性来研究其变化情况。结果表明:以萌发抗旱指数为标准指标,15个品种谷子萌发期抗旱性基本可以分为强、中、弱3类,其中抗旱性强的3个品种分别是陇谷10号、长生07、晋谷21号;抗旱性弱的2个品种是济谷20号、长生13。在干旱胁迫条件下谷子SOD、POD和CAT活性均有所升高,其中SOD活性在抗旱性强的3个品种中值最大,在抗旱性较弱的2个品种中值最小;SOD活性升高值与萌发抗旱指数显著正相关,而POD和CAT活性升高程度与萌发抗旱指数相关性不显著。通过相关性分析表明,活力抗旱指数、相对发芽率、相对发芽势与萌发抗旱指数呈极显著正相关,而相对胚芽长、相对胚根长与萌发抗旱指数之间没有明显的相关性。综上,在相关的六个抗旱性指标中,谷子抗旱的标准指标是萌发抗旱指数,谷子萌发期抗旱性鉴定的首要指标为活力抗旱指数和相对发芽率,次级指标则是相对发芽势,参考指标为相对胚芽长和相对胚根长。
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王永丽, 王珏, 杜金哲, 等. 不同时期干旱胁迫对谷子农艺性状的影响[J]. 华北农学报, 2012, 27(6):125-129.
采用盆栽控水方法,研究不同生育时期干旱胁迫对谷子物候期、株高、叶片数、顶叶叶面积、穗长、根轮数等农艺性状及产量的影响。结果表明,干旱延迟谷子物候期的出现;拔节期干旱对谷子株高的影响最大;干旱不会引起谷子总叶片数的改变,但对叶片发生时间有一定影响;拔节期干旱和孕穗期干旱使谷子顶叶叶面积减小;干旱抑制谷子根系的生长,但复水后生长迅速恢复。拔节期干旱对谷子农艺性状造成的影响最严重、最持久,干旱胁迫使谷子的产量降低,尤以灌浆期干旱对产量影响最显著。
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樊仙全, 杨绍林, 李如丹, 等. 甘蔗苗期抗旱性鉴定评价研究[J]. 中国农学通报, 2022, 38(3):17-24.
以12个甘蔗品种试验材料,通过桶栽试验,在苗期进行反复干旱胁迫处理,测定甘蔗幼苗存活率和叶片叶绿素含量、可溶性糖含量、丙二醛含量、超氧化物歧化酶活性等生理指标。运用抗旱系数法和隶属函数值法,对苗期甘蔗各生理指标性状进行了反复干旱胁迫下的抗性评价和鉴定,为甘蔗抗旱品种的选择提供可借鉴的方法,同时为其抗旱育种和蔗区抗旱品种示范推广提供数据支持。结果表明,反复干旱处理后,所有甘蔗品种幼苗存活率、叶片叶绿素含量、POD和SOD酶活性均有降低,而叶片Pro、REC、MDA和可溶性糖含量均明显增加,且各甘蔗品种间存在明显差异。‘LC 09-15’、‘GT 32’、‘FN 08-3214’、‘YZ 08-1609’、‘YZ 11-1204’、‘YZ 05-51’、‘YR 11-450’和‘DZ 12-88’等8个甘蔗品种的苗期抗旱性强于对照品种‘ROC 22’。通过甘蔗优异抗旱品种的筛选和推广,解决蔗区主栽甘蔗品种‘ROC 22’种性退化性状,可为蔗区有效抵御早春干旱、保证甘蔗稳产、高产提供理论指导。
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Fine roots are a key component of carbon (C) flow and nitrogen (N) cycling in forest ecosystems. However, the complexity and heterogeneity of the fine root branching system have hampered the assessment and prediction of C and N dynamics at ecosystem scales. We examined how root morphology, biomass, and chemistry differed with root branch orders (1-5 with root tips classified as first order roots) and how different root orders responded to increased C sink strength (via N fertilization) and reduced carbon source strength (via canopy scorching) in a longleaf pine (Pinus palustris L.) ecosystem. With increasing root order, the diameter and length of individual roots increased, whereas the specific root length decreased. Total root biomass on an areal basis was similar among the first four orders but increased for the fifth order roots. Consequently, total root length and total root surface area decreased systematically with increasing root order. Fine root N and lignin concentrations decreased, while total non-structural carbohydrate (TNC) and cellulose concentrations increased with increasing root order. N addition and canopy disturbance did not alter root morphology, but they did influence root chemistry. N fertilization increased fine root N concentration and content per unit area in all five orders, while canopy scorching decreased root N concentration. Moreover, TNC concentration and content in fifth order roots were also reduced by canopy scorching. Our results indicate that the small, fragile, and more easily overlooked first and second order roots may be disproportionately important in ecosystem scale C and N fluxes due to their large proportions of fine root biomass, high N concentrations, relatively short lifespans, and potentially high decomposition rates.
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Progress in understanding the network of mechanisms involved in maize primary root growth maintenance under water deficits is reviewed. These include the adjustment of growth zone dimensions, turgor maintenance by osmotic adjustment, and enhanced cell wall loosening. The role of the hormone abscisic acid (ABA) in maintaining root growth under water deficits is also addressed. The research has taken advantage of kinematic analysis, i.e. characterization of spatial and temporal patterns of cell expansion within the root growth zone. This approach revealed different growth responses to water deficits and ABA deficiency in distinct regions of the root tip. In the apical 3 mm region, elongation is maintained at well-watered rates under severe water deficit, although only in ABA-sufficient roots, whereas the region from 3-7 mm from the apex exhibits maximum elongation in well-watered roots, but progressive inhibition of elongation in roots under water deficit. This knowledge has greatly facilitated discovery of the mechanisms involved in regulating the responses. The spatial resolution with which this system has been characterized and the physiological knowledge gained to date provide a unique and powerful underpinning for functional genomics studies. Characterization of water deficit-induced changes in transcript populations and cell wall protein profiles within the growth zone of the maize primary root is in progress. Initial results from EST and unigene analyses in the tips of well-watered and water-stressed roots highlight the strength of the kinematic approach to transcript profiling.
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The objectives of this two-year field experiment were (1) to study the effect of irrigation frequency and irrigation amount on the root characteristics of drip-irrigated spring wheat (Triticum aestivum L.) and (2) to determine the relationship between these root characteristics and aboveground biomass and yield. A split-plot design was used with two wheat cultivars (Xinchun 6 and Xinchun 22). The irrigation treatments consisted of three irrigation intervals (D 13 d; D, 10 d; and D, 7 d) and three water amounts (W1, 3750 m/ha; W2, 6000 m/ha; and W3, 8250 m/ha). The results showed that root length density (RLD) and root weight density (RWD) were greater at 0-20 cm than at 20-40 cm at flowering. The RLD was greater in D1 and D2 than in D3 in the shallow soil layer and did not differ among the treatments with different irrigation frequencies in deep soil. The RLD at the 0-20 cm depth of W3 was 17.9% greater than that of W2 and 53.8% greater than that of W1, and the RLD trend was opposite at the 20-40 cm depth. The root-shoot ratio was significantly higher in D2 than in the other treatment, whereas the RLD, RWD, leaf Pn and LAI were significantly greater in D3. Leaf Pn and LAI both increased as the irrigation amount increased. Regression analysis showed a natural logarithmic relationship between RWD and aboveground biomass (R> 0.60, P < 0.05) and binomial relationships of the RWD at 0-20 cm depth (R = 0.43, P < 0.05) and the RLD at 20-40 cm depth (R = 0.34, P < 0.05) with grain yield. We found that with the optimum irrigation amount (W2), increasing drip irrigation frequency can increase wheat root length and root weight and aboveground biomass accumulation, thereby improving yield and water use efficiency.
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