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基于细胞色素B(Cyt b)基因对翘嘴鲌不同群体遗传多样性的分析
Culter alburnus Basilewsky in Different Populations: Genetic Diversity Analysis Based on Cytochrome B (Cyt b) Gene
为了解长江下游翘嘴鲌(Culter alburnus Basilewsky)不同群体遗传多样性水平,评估其资源状况。研究以淀山湖(DSH)、太湖(TH)、长荡湖(CD)和长江(CJ)4个水域共211尾翘嘴鲌为研究对象,对其线粒体DNA细胞色素b基因(Cyt b)序列进行测序分析,所得基因序列在NCBI中比对,运用MEGA、DNASP等软件分析得到4个翘嘴鲌群体的遗传多样性数据。翘嘴鲌Cyt b基因序列长度为1088 bp,其中碱基A+T含量为55%,G+C为45%。共检测到258个多态位点,占核苷酸总数的23.71%。4个群体211个样本中得到46个单倍型,单倍型多样性为0.849~0.946,具体表现为TH>CD>CJ>DSH。核苷酸多样性为0.0083~0.174,具体表现为DSH> CD>CJ>DSH。4个群体间遗传距离0.052~0.152,太湖群体和淀山湖群体的群体间遗传距离最大为0.152。群体间的遗传分化指数范围为0.197~0.646,基因流值范围为0.152~1.019,其中长江与淀山湖群体的基因流值最小,长江与长荡湖群体的基因流值最大。基于NJ法构建的系统进化树结果显示,4个群体翘嘴鲌没有明显地理区分。本研究结果表明,长江下游翘嘴鲌群体遗传多样性较高,结合近年来翘嘴鲌渔业资源的调查结果,以深入了解其遗传背景和种质资源现状,为翘嘴鲌的野生资源保护提供基础数据。
In order to understand the genetic diversity of different Culter alburnus Basilewsky populations in the lower reaches of the Changjiang River, and evaluate the resource status of C. alburnus. A total of 211 C. alburnus samples from Dianshan Lake (DSH), Taihu Lake (TH), Changdang Lake (CD) and Changjiang River (CJ) were studied. Mitochondrial DNA b gene (Cyt b) of C. alburnus was sequenced, and compared with NCBI. Genetic diversity data of four C. alburnus populations were analyzed by MEGA, DNASP and other software. The Cyt b gene sequence was 1088 bp in length, with 55% A+T and 45% G+C. A total of 258 polymorphic sites were detected, accounting for 23.71% of the total nucleotides. 46 haplotypes were found in 211 samples from 4 populations, and the haplotype diversity ranged from 0.849 to 0.946, the order was as following: TH>CD>CJ>DSH. The nucleotide diversity was 0.0083-0.174, which was represented as DSH> CD>CJ>DSH. The genetic distance between the four populations was 0.052-0.152, and the maximum was 0.152 between Taihu population and Dianshan Lake population. The genetic differentiation indices ranged from 0.197 to 0.646, and the gene flow values ranged from 0.152 to 1.019, among which the gene flow values of Changjiang River and Dianshan Lake populations were the smallest, while those of Changjiang River and Changdang Lake populations were the largest. The results of phylogenetic tree based on NJ method showed that there was no obvious geographical differentiation among the four populations. The results of this study indicated that the population genetic diversity of C. alburnus in the lower reaches of the Changjiang River was high. Combined with the results of fishery resources investigation in recent years, the genetic background and germplasm resources of C. alburnus in the lower reaches of the Changjiang were further understood, which provided basic data for wild resource conservation.
翘嘴鲌 / 线粒体DNA / 细胞色素b基因 / 遗传多样性 {{custom_keyword}} /
Culter alburnus Basilewsky / mitochondrial DNA / Cytochrome b gene / genetic diversity {{custom_keyword}} /
| [1] |
倪勇, 伍汉霖. 江苏鱼类志[M]. 北京: 中国农业出版社, 2006:284-285.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [2] |
姜建湖, 沈斌乾, 陈建明, 等. “太湖鲂鲌”及其亲本肌肉营养成分的分析与评价[J]. 水生生物学报, 2019, 43(2):388-394.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [3] |
吕大伟, 周彦锋, 葛优, 等. 淀山湖翘嘴鲌的年龄结构与生长特性[J]. 水生生物学报, 2018, 42(4):762-769.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [4] |
周彦锋, 吕大伟, 葛优, 等. 淀山湖河蚬翘嘴红鲌国家级水产种质资源保护区翘嘴鲌产卵场现状[J]. 长江流域资源与环境, 2018, 27(12):2733-2739.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [5] |
李停, 贾永义, 刘士力, 等. 二次正交旋转组合设计优化人工诱导翘嘴鲌雌核发育[J]. 中国水产科学, 2016, 23(1):77-89.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [6] |
刘月亮, 贾永义, 蒋文枰, 等. 雌核发育翘嘴鲌胚胎发育和形态特征的研究[J]. 上海海洋大学学报, 2017, 26(2):203-211.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [7] |
李忠利, 冉辉, 杨马, 等. 锦江翘嘴鲌的繁殖生物学特征[J]. 动物学杂志, 2017, 52(2):263-270.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [8] |
王哲, 刘春雷, 顾泽茂, 等. 多子小瓜虫的形态发生及其寄生导致翘嘴鲌鳃组织病理变化[J]. 水生生物学报, 2016, 40(5):935-941.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [9] |
杨子拓, 孙际佳, 李桂峰, 等. 基于线粒体D-LOOP基因的珠江翘嘴鲌遗传多样性与遗传分化研究[J]. 中山大学学报:自然科学版, 2016, 55(2):89-96.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [10] |
王伟, 陈立侨, 禹娜, 等. 应用COⅡ基因部分序列分析翘嘴鲌群体的遗传多样性[J]. 大连水产学院学报, 2008, 14(5):403-408.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [11] |
黄小彧. 长江水系翘嘴鲌遗传多样性研究[D]. 广州:暨南大学, 2012:30-42.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [12] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [13] |
杨金权, 刘焕章. 两种鲿科鱼类在长江和珠江流域Cytb基因序列变异性分析[J]. 水生生物学报, 2003, 27(3):253-257.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [14] |
吕国庆, 李思发. 鱼类线粒体DNA多态研究和应用进展[J]. 中国水产科学, 1998, 16(3):95-104.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [15] |
边力, 王鹏飞, 陈四清, 等. 基于线粒体Cytb基因序列的绿鳍马面鲀6个野生群体的遗传结构分析[J]. 中国水产科学, 2018, 25(4):125-134.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [16] |
王利华, 罗相忠, 王丹, 等. 基于COI和CytbDNA条形码在鲌属鱼类物种鉴定中的应用[J]. 淡水渔业, 2019, 49(4):22-28.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [17] |
王伟. 翘嘴鲌(Culteral burnus)群体遗传多样性及鲌亚科鱼类系统发生的研究[D]. 上海:华东师范大学, 2007:38-83.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [18] |
何玮. 长荡湖渔业生态环境评估及资源增殖对策[D]. 南京:南京农业大学, 2015:1-6.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [19] |
DnaSP is a software package for a comprehensive analysis of DNA polymorphism data. Version 5 implements a number of new features and analytical methods allowing extensive DNA polymorphism analyses on large datasets. Among other features, the newly implemented methods allow for: (i) analyses on multiple data files; (ii) haplotype phasing; (iii) analyses on insertion/deletion polymorphism data; (iv) visualizing sliding window results integrated with available genome annotations in the UCSC browser.Freely available to academic users from: (http://www.ub.edu/dnasp).
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [20] |
We present here a new version of the Arlequin program available under three different forms: a Windows graphical version (Winarl35), a console version of Arlequin (arlecore), and a specific console version to compute summary statistics (arlsumstat). The command-line versions run under both Linux and Windows. The main innovations of the new version include enhanced outputs in XML format, the possibility to embed graphics displaying computation results directly into output files, and the implementation of a new method to detect loci under selection from genome scans. Command-line versions are designed to handle large series of files, and arlsumstat can be used to generate summary statistics from simulated data sets within an Approximate Bayesian Computation framework.© 2010 Blackwell Publishing Ltd.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [21] |
张久盘, 刘哲, 张波, 等. 祁连山裸鲤和青海湖裸鲤mtDNACytb基因和D-LOOP区序列特征及遗传分化[J]. 淡水渔业, 2015, 45(4):92-95.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [22] |
冯晓宇, 谢楠, 冯建彬, 等. 基于细胞色素b基因序列的鲌亚科鱼类系统发育研究[J]. 淡水渔业, 2009, 39(5):23-27.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [23] |
张力文, 田辉伍, 陈大庆, 等. 长江上游中华纹胸鮡遗传多样性及遗传结构研究[J]. 淡水渔业, 2020, 50(4):3-11.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [24] |
徐丹丹. 基于微卫星标记和线粒体cytb基因序列的鲇遗传多样性研究[D]. 重庆:西南大学, 2013:51-80.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [25] |
徐宇, 钟立强, 李潇轩, 等. 5个湖泊河川沙塘鳢(Odontobutis potamophila)种群线粒体细胞色素b基因的遗传变异分析[J]. 湖泊科学, 2015, 4(27):693-699.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [26] |
沙航, 罗相忠, 李忠, 等. 基于COI序列的长江中上游鲢6个地理群体遗传多样性分析[J]. 中国水产科学, 2018, 25(4):783-792.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [27] |
闫春梅, 杜晓燕, 郑伟, 等. 中国东北部山区细鳞鱼4个群体遗传多样性分析[J]. 水产科学, 2017, 36(6):778-783.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [28] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [29] |
陈会娟, 刘明典, 汪登强, 等. 长江中上游4个鲢群体遗传多样性分析[J]. 淡水渔业, 2018, 48(1):20-25.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [30] |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| [31] |
The red panda (Ailurus fulgens) is one of the flagship species in worldwide conservation and is of special interest in evolutionary studies due to its taxonomic uniqueness. We sequenced a 236-bp fragment of the mitochondrial D-loop region in a sample of 53 red pandas from two populations in southwestern China. Seventeen polymorphic sites were found, together with a total of 25 haplotypes, indicating a high level of genetic diversity in the red panda. However, no obvious genetic divergence was detected between the Sichuan and Yunnan populations. The consensus phylogenetic tree of the 25 haplotypes was starlike. The pairwise mismatch distribution fitted into a pattern of populations undergoing expansion. Furthermore, Fu's F(S) test of neutrality was significant for the total population (F(S) = -7.573), which also suggests a recent population expansion. Interestingly, the effective population size in the Sichuan population was both larger and more stable than that in the Yunnan population, implying a southward expansion from Sichuan to Yunnan.
{{custom_citation.content}}
{{custom_citation.annotation}}
|
| {{custom_ref.label}} |
{{custom_citation.content}}
{{custom_citation.annotation}}
|
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