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PDA中水含量对固体培养黄绿木霉T1010 分生孢子生长发育的影响
Water Content in PDA: Influence on Growth and Development of Trichoderma aureoviride 1010 Conidia on Solid Medium
本研究旨在探索马铃薯葡萄糖培养基(PDA)中水含量对固体培养黄绿木霉T1010分生孢子生长发育的影响,为木霉菌剂工厂化生产提供理论与技术支撑。以PDA为基本培养基,设计17种水营养配比和16种水琼脂配比,观测平板培养T1010的菌落形态、半径,菌丝生长状况和分生孢子发育过程、形态及其数量。在水葡萄糖质量比试验中,水葡萄糖质量比55.56时T1010分生孢子近似直径最大,比标准PDA提高7.94%;水葡萄糖质量比83.33时T1010菌丝生长极限值最大;水葡萄糖质量比500时比生长速率最大,为0.316/h;水葡萄糖质量比25时单位面积产孢量最大,比标准PDA提高61.04%;水葡萄糖质量比55.56时单位葡萄糖质量产孢量最大,比标准PDA提高10.86%。在水琼脂质量比试验中,水琼脂质量比38.46时单位面积产孢量最大,比标准PDA提高21.72%;水琼脂质量比500时单位琼脂质量产孢量最大,比标准PDA提高381.94%。PDA培养基中水、马铃薯、葡萄糖、琼脂配比为1000: 36:18: 26时,T1010分生孢子发育好、产量高,固体发酵生产中基质水调控很关键。
The purpose is to clarify the influence of water content in potato glucose medium (PDA) on growth and development of Trichoderma aureoviride 1010 conidia, and to provide theoretical and technical support for industrial production of Trichoderma agent. In this study, potato glucose medium (PDA) was used as the basic medium with 17 kinds of water/nutrition and 16 kinds of water/agar to be inoculated with T. aureoviride 1010. Colony morpha and radius, hyphal growth, the development, morpha and quantity of conidial of T1010 were observed. In the mass ratio of water/glucose test, the largest approximate diameter was at the water/glucose mass ratio of 55.56, which was 7.94% higher than that in standard PDA. The growth limit value of T1010 at the water/glucose mass ratio of 83.33 was the highest. The maximum specific growth rate was 0.316/h at the water/glucose mass ratio of 500. The highest conidia yield per unit area of medium was at the water/glucose mass ratio of 25, and the conidia yield increased by 61.04% than that in standard PDA. The highest conidia yield per unit mass of glucose was at the water/glucose mass ratio of 55.56, and the conidia yield increased by 10.86% than that in standard PDA. In the mass ratio of water/agar test, the conidia yield per unit area of medium at the water/agar mass ratio of 38.46 was 21.72% higher than that of standard PDA medium, and the conidia yield per unit mass of agar at the water/agar mass ratio of 500 was significantly higher than that of standard PDA by 381.94%. When the ratio of water, potato, glucose and agar in PDA medium was 1000:36:18:26, conidia of T1010 developed well and produced high yield. Thus, matrix water regulation is the key point in solid fermentation production.
PDA / 水含量 / 固体培养 / 相对含量 / 黄绿木霉 / 分生孢子 / 影响 {{custom_keyword}} /
PDA / water content / solid culture / relative content / Trichoderma aureoviride / conidia / influence {{custom_keyword}} /
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