为对毛细管荧光电泳检测过程中出现的非特异性峰进行观察，探讨其形态及发生原因，笔者从新疆野扁桃的5 个居群中分别挑选2 份叶片提取基因组DNA，PCR产物使用毛细管电泳荧光检测。结果表明：在毛细管电泳荧光检测中，共选用4 种荧光引物，其中荧光引物FAM峰型最为正常且特异峰清晰，不常发生非特异性峰。与之相反，荧光引物ROX的非特异峰种类数量最多，且特异峰不易辨认。在6 种异常情况中，N+1 峰出现次数最多，高低峰出现次数最少。荧光引物FAM更适合于新疆野扁桃进行扩增。
Abstract
The paper aims to observe the non-special peak appeared in capillary fluorescent electrophoresis and discuss its shape and the occurrence reason. We selected 2 samples of leaves from 5 populations of wild almond respectively to extract the genome DNA, and analyzed the amplification production by using the capillary fluorescent electrophoresis. The results showed that: there were 4 kinds of fluorescent primers in the capillary fluorescent electrophoresis; the peak of fluorescent primer FAM was within a normal range, with the special peak being obvious and the non-special peak did not occur frequently; by contrast, the non-special peak of fluorescent primer ROX had the most varieties, and the special peak was difficult to discern; in 6 exceptional cases, N+1 peak occurred the most and different peaks occurred the least. The fluorescent primer FAM is more suitable for wild almond to amplify.
关键词
新疆野扁桃;毛细管荧光电泳;TP-M13-SSR
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Key words
wild almond(Prunus tenella Batsch.); Capillary fluorescent electrophoresis; TP-M13-SSR
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