过量表达OsCATb提高大肠杆菌对不同重金属逆境胁迫的耐受性

刘大丽,马龙彪,鲁振强

中国农学通报. 2018, 34(34): 36-41

中国农学通报 ›› 2018, Vol. 34 ›› Issue (34) : 36-41. DOI: 10.11924/j.issn.1000-6850.casb18070019
生物技术科学

过量表达OsCATb提高大肠杆菌对不同重金属逆境胁迫的耐受性

  • 刘大丽,马龙彪,鲁振强
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Over-expression of OsCATb Enhanced E. coli Stress Tolerance to Heavy Metals

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摘要

为了进一步研究水稻Catalase b (OsCATb)在重金属逆境胁迫下的作用机制，笔者将外源表达重组质粒pGEX-6p-3-OsCATb转化到大肠杆菌BL21中。SDS-PAGE表明，通过IPTG的诱导表达，在53 kDa的位置获得了与推测分子量大小相一致的可溶性目的蛋白。在1 mmol/L Cd、Zn和Cu的重金属逆境胁迫下，过量表达GST-OsCATb 的重组菌表现出优于对照菌株(BL21)以及过量表达标签蛋白GST的空载体菌株的耐受性；同时，目的基因重组菌体内的过氧化氢酶活性也要远高于实验对照组。研究结果也进一步暗示了水稻OsCATb 作为重要的抗氧化剂，能够参与到细胞的重金属逆境胁迫应答、解毒和耐受机制过程中。

Abstract

The paper aims to study the mechanism of Oryza sativa Catalase b (OsCATb) in heavy metal stress. We introduced the exogenous reconstructed plasmid pGEX-6p-3-OsCATb into E. coli BL21. SDS-PAGE showed that there was a soluble protein band in 53 kDa in accordance with its predicted molecular weight. Under 1 mM Cd2+, Zn2+ or Cu2+ heavy metal stress, strain over-expressing GST-OsCATb exhibited better growth and increased tolerance than control (BL21) and E. coli over-expressing GST tag; meanwhile, the catalase activities in aimgene recombinant strain were also higher than the other two controls’. The results suggest that rice OsCATb appears to be an important antioxidant in cellular response, detoxification and tolerance processes under heavy metal stress.

关键词

重金属逆境;过氧化氢酶;大肠杆菌;OsCATb基因

Key words

heavy metal stresses; catalase; E. coli; OsCATb gene

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刘大丽,马龙彪,鲁振强. 过量表达OsCATb提高大肠杆菌对不同重金属逆境胁迫的耐受性. 中国农学通报. 2018, 34(34): 36-41 https://doi.org/10.11924/j.issn.1000-6850.casb18070019
Over-expression of OsCATb Enhanced E. coli Stress Tolerance to Heavy Metals. Chinese Agricultural Science Bulletin. 2018, 34(34): 36-41 https://doi.org/10.11924/j.issn.1000-6850.casb18070019

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