应用高通量测序技术(RNA-seq),获得显著差异表达基因369条,160条基因显著下调,209条基因显著上调。其中显著差异表达基因在ECM与受体相互作用信号通路和MAPK信号通路中富集明显。分析发现,灭多威对精巢组织细胞粘附性造成影响,诱导ECM与受体相互作用中相关基因表达显著上调,如层粘连蛋白Laminins、整合素ɑ6、β1等,以稳定精巢组织结构；JNK途径中MAP3K、MKK4、JNK显著表达上调,增强录因子AP-1,促进 P53、Bax等促凋亡蛋白的表达；MAPK信号通路ERK途径中相关生长因子表达受到影响,从而可能对罗非鱼精巢组织生殖细胞增殖产生影响。
Abstract
369 significantly expressed genes were obtained by RNA sequencing(RNA-Seq). 160 genes were significantly down- regulated, and 209 genes were up-regulated. The expression of the differentially expressed genes in MAPK signaling pathway and ECM-receptor interaction was more significant. The results indicated that the adhesion of Testis tissue cells was influenced by Methomy stress, that induced the expression of related gene in ECM-receptor interaction to up-regulated for stabilizing the structure of testis tissue, such as Laminins, integrin alpha 6 and integrin beta 1. JNK, MAP3K and MKK4 in JNK pathway were significantly up-regulated, this enhanced transcription factor AP-1 to promote the expression of P53, Bax and other pro-apoptotic proteins. Because the expression of related growth factors in the ERK pathway of MAPK signaling pathway was significantly down-regulated by methomy stress, the proliferation of reproduction cells in the testis tissue may be affected.
关键词
灭多威;罗非鱼;精巢;转录组;信号通路
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Key words
Methomyl; Tilapia; Testis; Transcriptome; Signaling pathway
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