用鸡胚培养法和血凝试验,测定了鱼腥草对鸡新城疫病毒的抑制作用。试验共分为4组,第1组接种病毒尿囊液与中药鱼腥草混合液,HA滴度0。第Ⅱ组接种新城疫病毒尿囊液与盐酸金刚烷胺混合液,HA滴度5 log2。第Ⅲ组接种新城疫病毒尿囊液与生理盐水混合液,HA滴度9log2。第Ⅳ组不接种病毒尿囊液,不给药,HA滴度0。试验结果表明：鱼腥草对鸡胚无毒性作用；鱼腥草与病毒同时接种鸡胚,能完全抑制鸡新城疫病毒在鸡胚中的增殖。
Abstract
To study whether the traditional Chinese herb medicine Houttuynia cordata can inhibit the proliferation of Newcastle disease virus, we applied chick embryo culture and hemagglutination test. The experiment was divided into four groups, the embryos in group Ⅰ were inoculated with the allantoic fluid containing virus and H. cordata extract, those in group Ⅱ were inoculated with the allantoic fluid containing virus and amantadine hydrochloride, those in group Ⅲ were inoculated with the allantoic fluid containing virus and normal saline mixture, and those in group Ⅳ were not inoculated with virus or drug. The results showed that the titer of group Ⅰ, Ⅱ, Ⅲ, and Ⅳ was 0, 5 log2, 9 log2, and 0, respectively, indicating that H. cordata extract had no toxic effect on chick embryo, and could completely inhibit the proliferation of Newcastle disease virus in chick embryo.
关键词
鱼腥草;鸡新城疫病毒;鸡胚接种;血凝试验
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Key words
Houttuynia cordata; Newcastle disease virus; SPF chicken embryo inoculation; Hemagglutination test
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参考文献
[1] 丁 壮,尹仁福,薛 聪,等．新城疫流行病学新特点及鹅新城疫防控策略[J]．中国兽医学报,2015,35(1)：159-168．
[2] 王学理,鄢长庆,刘珂飞,等.莪术油注射液鸡胚接种抗新城疫病毒的研究[J].江苏农业学报,2012,28(3)：683-684．
[3] 李秀清．中药鱼腥草的现代药理研究[J]．黑龙江医药,2014,27(4)：865-868．
[4] 张志荣,沈淼山,吴文辉. 鱼腥草总多酚提取工艺的优化及不同产地含量比较[J].中华医药学刊,2016,34(10)：2445-2448．
[5] 陈 婧,方建国,吴方建,等．鱼腥草抗炎药理作用机制的研究进展[J].中草药,2014,45( 2) : 284-289．
[6] Chen Y F,Yang J S,Chang W S,et al．Houttuynia cordata Thunb extract modulates G0 / G1 arrest and Fas / CD95-mediated death receptor apoptotic cell death in human lung cancer A549 cells[J]. J Biomed Sci,2013,20 ( 18 ) : 1-8．
[7] 潘 颖,魏亚超,王 乐 等. 鱼腥草滴眼液治疗流行性角结膜炎的临床疗效及经济效益学分析[J]. 现代药物与临床,2013,28(4): 562-566.
[8]杨文凡,陈 勇,程翼宇．鱼腥草不同部位挥发油成分的研究[J]. 中草药,2006,37(8): 1149-1151.
[9] Wang Z M,Zhu X X,Cui X L,et al. Screeningof traditional Chinese remedies for SARS treatment [J]. China J Chin Mater Med,2003,28(6)：484-487.
[10] 刘秀梵,胡顺林．新城疫病毒的进化及其新型疫苗的研制[J].中国兽药杂志,2010,(1)：12-18.
[11] Chen X Q,Wang C Y,Xu L F,et al． A Laboratory Evaluation of Medicinal Herbs Used in China for the Treatment of Hand,Foot,and Mouth Disease[J]．Evidence-Based Complementary and Alternative Medicine,2013 (2013), Article ID 504563,10 pages．
[12] Lin T Y,Liu Y C,Jheng J R,et al．Anti-enterovirus 71 activity screening of Chinese herbs with anti-infection and inflammation activities[J]．Am J Chin Med,2009,37( 1) : 143-158．
[13] 吴德峰,陈绍军,翁顺太,等. 鱼腥草抗病毒合剂对鸡法氏囊病的疗效及小鼠的免疫作用[J]. 福建农林大学学报(自然科学版),2003,32(2)：221-224.
[14] 李 丹,李 力,张柳红. 鱼腥草有效成分抗巨细胞病毒的实验研究[J].中国妇幼保健,2010,25(36)：5463-5465.
[15] 王 健,史 玉,张永泽,等. 鱼腥草不同部位提取物的抗菌抗病毒作用比较[J].河北工 程大学学报(自然科学版),2010,27(2)：104-106.
[16] Yin J C,Li G X,Li J,et al．In vitro and in vivo effects of Houttuynia cordata on infectious bronchitis virus[J]．Avian Pathol,2011,40(5)：491-498．
[17] Ren X F,Sui X W,Yin J C． The effect of Houttuynia cordata injection on pseudorabies herpesvirus( Pr V) infection in vitro[J]．Pharm Biol,2010,49(2)：161-166．
[18] 樊宏伟,瞿 卫,立 彦,等. 鱼腥草黄酮提取物对肿瘤细胞的抑制作用[J].中国医院药学杂志,2008,28(7)：528-531.
[19] 薛兴阳,吴华振,付腾飞,等.鱼腥草生物碱抑制人大细胞肺癌细胞生长研究[J].现代中西医结合杂志,2016,25(27)：2972-2975.
[20] 张壮丽,赵 宁,赵志鸿,等. 鱼腥草挥发油抗淋巴瘤细胞谱效关系[J].2015,50(3)：378-381.
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脚注
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