旨在优化植物根内生菌高通量测序分析技术，更好地解析健康与患根肿病上海青根内生菌之间的差异。采用高通量测序方法，对其16S rDNA的V3-V4 区进行扩增，并通过合适的软件和数据库进行菌群分析。结果表明，植物内生菌受植物叶绿体DNA的干扰大，平均叶绿体数据占67%。上海青根内生微生物菌群具有丰富的多样性，主要由变形菌门、放线菌门、拟杆菌门和厚壁菌门细菌组成，优势种群为假单胞菌，根瘤菌，芽孢杆菌，以及链霉菌等。健康样品的稳定性较好，不同患根肿病样品之间菌群比例差异较大。研究显示，根肿病侵染对上海青根内生菌群的结构和组成有较为显著的影响。
Abstract
The study aims to optimize high- throughput sequencing analysis techniques of vegetable root endophytes and get a better understanding of the difference in root endophytic bacteria between healthy and clubroot-infected Brassica chinensis L.. The V3-V4 region of 16S rDNA was amplified by high-throughput sequencing method and the data was analyzed by appropriate software and databases. The results showed that the plant endophytes were disturbed by chloroplast DNA severely, the average chloroplast data accounted for 67%. B. chinensis root endophytes had rich diversity, and Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes were primary endophytic groups. The dominant endophytic groups were Pseudomonas, Rhizobium, Bacillus, and Streptomyces. The stability of healthy samples was better than clubroot-infected samples, and the difference of clubroot- infected samples was significant. The study indicated that clubroot infection had a significant effect on the structure and composition of B. chinensis root endophytic bacteria.
关键词
上海青;根内生菌群;宏基因组学;分析方法
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Key words
Brassica chinensis L.; root endophytes; clubroot disease; metagenomics; analytical method
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