以中国柽柳为试材,通过盆栽试验,研究混合碱(NaHCO3和Na2CO3摩尔比为9:1)在不同浓度胁迫下(100、200、300和400 mmol/L)对中国柽柳幼苗叶片生理特性和株高的影响。结果表明：与对照相比,中国柽柳叶片的相对含水量在碱浓度为100、200、300和400 mmol/L时均显著降低,分别下降8.82%、34.49%、43.27%和45.43%；而叶片丙二醛、可溶性糖和脯氨酸含量则随着碱浓度的升高呈递增的趋势,其中100、200、300和400 mmol/L碱处理的脯氨酸含量分别较对照显著高出36.37%、56.41%、77.35%和84.25%。此外,不同碱浓度处理的株高大小次序为：对照>100 mmol/L>200 mmol/L>300 mmol/L≈400 mmol/L,其中100、200、300和400 mmol/L处理的株高分别比对照显著降低45.95%、62.16%、70.27%和74.05%,表明不同程度的碱胁迫均显著抑制了中国柽柳的生长。综合分析认为,以NaHCO3和Na2CO3为主要盐分的碱胁迫对中国柽柳的生长发育具有一定的抑制作用,随着碱胁迫程度的加剧,抑制作用越来越大。
Abstract
A pot experiment was conducted to determine the effects of mixedalkali (the mole ratio of NaHCO3 and Na2CO3 is 9:1) at different concentrations of 100, 200, 300, and 400 mmol/L mixtures on physiological characteristics in leaves and plant height of TamarixchinensisLour. seedlings. The results showed that in comparison with the control, the relative water contents in leaves of TamarixchinensisLour. were significantly decreased at the alkali concentrations of 100, 200, 300, and 400 mmol/L by 8.82%, 34.49%, 43.27%, and 45.43%, respectively. Whereas the contents of MDA, soluble sugar and proline presented an increasing trend along with the increase of alkali concentrations, and the proline contents in the alkali concentrations of 100, 200, 300, and 400 mmol/L were increased by 36.37%, 56.41%, 77.35%, and 84.25%, respectively, compared with the control. In addition, the size order of plant height in different alkali concentrations was as follows: control>100 mmol/L>200mmol/L>300mmol/L≈400mmol/L, and the plant height in the alkali concentrations of 100, 200, 300, and 400 mmol/L were significantly decreased by 45.95%, 62.16%, 70.27%, and 74.05% in contrast to the control, respectively. This suggested that different degree of alkali stress equally inhibited the growth of TamarixchinensisLour.seedlings. As a result, the alkali stress mainly including NaHCO3 and Na2CO3 had significantly inhibitory effect on the growth of amarixchinensisLour., and as the increase of degree of alkali stress, the inhibitory effect become larger and larger.
关键词
中国柽柳;碱胁迫;相对含水量;可溶性糖;脯氨酸
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Key words
TamarixchinensisLour.; alkali stress; relative water content; soluble sugar; proline
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