高效液相色谱法测定黄皮果实中的有机酸

朱敏,何书强,罗石荣,麦贤家,陈业渊

中国农学通报. 2016, 32(34): 187-192

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PDF(633 KB)
中国农学通报 ›› 2016, Vol. 32 ›› Issue (34) : 187-192. DOI: 10.11924/j.issn.1000-6850.casb16080148
食品 营养 检测 安全

高效液相色谱法测定黄皮果实中的有机酸

  • 朱敏,何书强,罗石荣,麦贤家,陈业渊
作者信息 +

Determination of Organic Acids in Wampee [Clausena lansium (Lour.)Skeels] Fruits by High Performance Liquid Chromatography (HPLC)

  • Zhu Min, He Shuqiang, Luo Shirong, Mai Xianjia, Chen Yeyuan
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摘要

为深入了解黄皮果实中有机酸,采用水浴超声提取果实中的有机酸、高效液相色谱法测定的方法,对12份黄皮种质果实中有机酸的组分及含量进行研究。结果表明:在色谱柱Venusil XBP C18(T) (4.6 mm×250 mm,5 μm)、流动相为0.01 mol/L (NH4)2HPO4(H3PO4调pH至2.7)、流速1 mL/min、柱温27℃、检测波长210 nm的条件下,黄皮果实中柠檬酸、草酸、乳酸、苹果酸、抗坏血酸、酒石酸得到良好分离,且测定精确灵敏、回收率高、重复性好,标准曲线相关系数为0.9975~0.9996,相对标准偏差为1.02%~1.84%,回收率为95.60%~101.78%。黄皮果实中有机酸以柠檬酸、草酸和苹果酸为主,乳酸在少部分种质中有测出,不同种质果实中柠檬酸、草酸、苹果酸含量分别为0.780~8.090、0.773~4.205、0.478~3.212 mg/(g.FW),分别占总酸的22%~65%、16%~40%、11%~20%。

Abstract

To study the organic acids in wampee fruits, the constituent and content of organic acid in 12 wampee germplasms were analyzed by ultrasonic-assisted water extraction and high performance liquid chromatography (HPLC). The results show that six organic acids can be successfully separated and all the calibration curves for these organic acids display a good linear relationship with correlation coefficients of 0.9975-0.9996, RSD is 1.02%-1.84% (n=5) and recovery rate is 95.60%-101.78%, on the condition that Venusil XBP C18(T) column (4.6 mm×250 mm, 5 μm) is used at 27℃, mobile phase is 0.01 mol/L (NH4)2HPO4 (pH 2.6) with flow rate of 1 mL/min and detection wavelength of 210 nm. The major parts of organic acids in wampee fruits are citric acid, oxalix acid and malic acid, lactic acid is detected only in a few samples; in different samples, the content of citric acid, oxalic acid and malic acid is 0.780-8.090 mg/(g.FW), 0.773-4.205 mg/(g.FW), 0.478-3.212 mg/(g.FW), respectively, and accounts for 22%-65%, 16%-40% and 11%-20% of the total acid.

关键词

黄皮;高效液相色谱法;有机酸

Key words

wampee; high performance liquid chromatography (HPLC); organic acid

引用本文

导出引用
朱敏,何书强,罗石荣,麦贤家,陈业渊. 高效液相色谱法测定黄皮果实中的有机酸. 中国农学通报. 2016, 32(34): 187-192 https://doi.org/10.11924/j.issn.1000-6850.casb16080148
Zhu Min,He Shuqiang,Luo Shirong,Mai Xianjia and Chen Yeyuan. Determination of Organic Acids in Wampee [Clausena lansium (Lour.)Skeels] Fruits by High Performance Liquid Chromatography (HPLC). Chinese Agricultural Science Bulletin. 2016, 32(34): 187-192 https://doi.org/10.11924/j.issn.1000-6850.casb16080148

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