为比较B型烟粉虱和温室白粉虱分别取食烟草后对烟草生长特性的影响，明确2 种粉虱取食诱导的烟草防御反应的差异和时间效应，探讨B型烟粉虱诱导的烟草防御反应在竞争取代温室白粉虱中的作用，采用直尺测量和烘箱烘干称重的方法，研究比较B型烟粉虱和温室白粉虱分别取食烟草不同时间后对烟草自身生长指标影响的差异。结果表明：在诱导寄主防御的虫口密度下，B型烟粉虱和温室白粉虱分别取食烟草不同时间后对烟草自身生长的影响存在显著差异，且具有时间效应。与对照烟草相比，B型烟粉虱取食烟草5 天后对烟草生长没有明显的不利影响，但从取食10 天开始出现显著不利影响，随着取食时间的延长对烟草的不利影响逐渐增加。烟粉虱取食15、20 天后烟草株高分别降低了6.9、8.5 cm，平均节间距分别缩短了0.6、0.53 cm，叶片数量分别减少了2.1、2.6 叶/株，系统叶叶面积明显增大，叶片变薄，单位面积干重分别降低了0.99、1.01 mg/cm2。温室白粉虱取食烟草不同时间后对烟草生长没有明显的不利影响。与温室白粉虱为害烟草后相比，B型烟粉虱为害烟草后产生了对烟草的特异性抑制反应，这可能与B型烟粉虱诱导烟草表达的特异性防御反应对自身资源的消耗有关。
Abstract
The paper objective is to compare the effect of growth characteristic of tobacco after feeding by Bemisia tabaci Biotype B and Trialeurodes vaporariorum respectively on tobacco plants, and to determine the difference defense response of tobacco plants and temporal effect by two whitefly induced, and to explore its role in interspecific competition between B. tabaci and T. vaporariorum and its possible mechanism. The difference effects of growth characteristic of tobacco after feeding different times by B. tabaci and T. vaporariorum respectively get though using ruler measurement and oven drying weighing method. The results showed that the significant difference of tobacco growth and exist temporal effect after feeding by B. tabaci and T. vaporariorum, respectively, under the whitefly population density of reached plant induced defense. Compared with the control of tobacco, the tobacco feeding by B. tabaci had no negative effects on growth for 5 d, and but had negative effects on plant growth start for 10 d after feeding by B. tabaci and had gradually increase the adverse effects of tobacco follow the extension of B. tabaci feeding time. The tobacco plants height were decreased by 6.9 cm and 8.5 cm, and the average internode were decreased by 0.6cm and 0.53cm, and the leaf number of tobacco plants was decreased by 2.1 leaf/plant and 2.6 leaf/plant, and the dry weight of per unit areas were decreased by 0.99 mg/cm2 and 1.01 mg/cm2 after previous feeding by B. tabaci for 15 d and 20 d, respectively. The tobacco feeding different times by T. vaporariorum had no negative effects. Compared with tobacco after feeding by T. vaporariorum, B. tabaci feeding tobacco can induce specific inhibition responses, B. tabaci -elicited declines in growth may result from the re-allocation of resources from growth to defense.
关键词
烟草；生长特性；B型烟粉虱；温室白粉虱；诱导防御
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Key words
tobacco; growth characteristics; Bemisia tabaci B-biotype; Trialeurodes vaporariorum; induced defense
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