贵州省甘蔗审定品种及区试材料的SSR-CE/FD分析

付瑜华,Pan Yong-Bao,雷朝云,谢慧珏,雷石富,卢加举

中国农学通报. 2017, 33(8): 5-12

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中国农学通报 ›› 2017, Vol. 33 ›› Issue (8) : 5-12. DOI: 10.11924/j.issn.1000-6850.casb16050053
农学 农业基础科学

贵州省甘蔗审定品种及区试材料的SSR-CE/FD分析

  • 付瑜华,Pan Yong-Bao,雷朝云,谢慧珏,雷石富,卢加举
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SSR-CE/FD Assessment of Guizhou Approved Sugarcane Cultivars and Regional Materials

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摘要

旨在保护贵州育种单位知识产权，指导当地甘蔗杂交育种亲本组合的制定。采用SSR标记和毛细管电泳/荧光检测技术相结合(SSR-CE/FD)的技术，对12 个贵州甘蔗材料进行基因型分析，利用NTsys软件进行数据处理。21 对多态性高的SSR引物共获得131 条扩增带，多态性条带比例为90.1%，平均每对引物产生6.2 个条带。其中11 个条带为7 个品种（系）中某一品种（系）的特征条带；利用这些特征条带可以迅速将该7 个品种（系）的中某一品种（系）与其他材料区分开。引物SMC31CUQ多态性条带比例为100%，且可以将12 个甘蔗材料完全区分开，是分辨率最高的引物。UPGMA聚类分析表明12 个甘蔗无性系间的遗传相似系数变异范围为0.64~0.92。本研究成功构建了贵州3 个审定品种和9 个区试材料基于131 个SSR条带的指纹图谱，12 个甘蔗无性系之间遗传基础较为狭窄，育种工作中应选用遗传背景差异大的甘蔗亲本，从而拓宽贵州甘蔗品种（系）的遗传基础。

Abstract

mbination in local sugarcane cross breeding, SSR-capillary electrophoresis/fluorescence detection (SSR-CE/FD) technology was used to analyze twelve sugarcane genotypes from Guizhou. The SSR data were then calculated using NTsys software. A total of 131 DNA fragments were amplified through PCR by 21 pairs of highly polymorphic SSR primers, of which 90.1% were polymorphic. The number of fragments per primer pair was 6.2. Eleven fragments were genotype-specific fragment of one cultivar (line) from seven cultivars (lines); these genotype-specific fragments could distinguish one cultivar (line) from other six cultivars (lines). The SMC31CUQ primer pair, produced 100% polymorphic alleles, was the highest-resolution primer that could distinguish all the 12 sugarcane genotypes. According to the cluster results of UPGMA program, pairwise similarity coefficients among the 12 molecular identities varied from 0.64 to 0.92. Our study successfully constructed a SSR fingerprint of 131 DNA fragments about 3 cultivars and 9 regional materials in Guizhou Province. The twelve sugarcane genotypes have a narrow genetic base, indicating parental materials with big differences in genetic background should be employed in sugarcane breeding programs in order to broaden the genetic basis among sugarcane cultivars (clones) in Guizhou Province.

关键词

贵州；甘蔗；SSR-CE/DF；指纹图谱

Key words

Guizhou; sugarcane; SSR-CE/FD; fingerprints

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付瑜华,Pan Yong-Bao,雷朝云,谢慧珏,雷石富,卢加举. 贵州省甘蔗审定品种及区试材料的SSR-CE/FD分析. 中国农学通报. 2017, 33(8): 5-12 https://doi.org/10.11924/j.issn.1000-6850.casb16050053
SSR-CE/FD Assessment of Guizhou Approved Sugarcane Cultivars and Regional Materials. Chinese Agricultural Science Bulletin. 2017, 33(8): 5-12 https://doi.org/10.11924/j.issn.1000-6850.casb16050053

参考文献

[1] 梅洪娟,马瑞君,庄东红. 指纹图谱技术及其在植物种质资源中的应用[J].广东农业科学, 2014, 3:159-164
[2] 辜大川,邱东峰,殷明珠,等.水稻优异资源分子身份证的建立[J].湖北农业科学,2012,51(24):5579-5589
[3] 王如琼,余显权,姜敬伟,等.贵州省2011年水稻区试品种DNA指纹图谱的构建及遗传多样性分析[J].种子,2012,31(4):35-39
[4] 张志勇,詹先进,蓝家样,等.利用SSR标记鉴定棉花品种和纯度研究进展[J].湖北农业科学,2013,52(9):1992-1994
[5] 尤春源,聂新辉,张胜,等.新疆彩色棉23个品种指纹图谱的构建及遗传多样性分析[J].棉花学报,2014,26(2):161-170
[6] 谭君,丁仲芳,孙仕贤,等.西南常用玉米自交系SSR指纹图谱构建[J].西南农业学报,2003,16(2):1-6
[7] 刘涛,胡萍,赵德刚,等.黔东南地方玉米品种SSR分子标记指纹图谱构建[J].种子,2010,29(9):24-29
[8] 徐海风,程保山,杨加银,等.黄淮海地区夏大豆品种(系)指纹图谱的构架及其遗传多样性分析[J].西南农业学报,2014,27(5):1814-1819
[9] 苗晗,张圣平,顾兴芳,等.中国黄瓜主栽品种SSR遗传多样性分析及指纹图谱构建[J].植物遗传资源学报,2014,15(2):333-341
[10] 邱杨,李锡香,李清霞,等.利用SSR标记构建萝卜种质资源分子身份证[J].植物遗传资源学报,2014,15(3):648-654
[11] 滕海涛,吕波.利用DNA指纹图谱辅助植物新品种保护的可能性[J].生物技术通报, 2009,1:1-6
[12] 周云龙.当品种管理遇上DNA[J].种子科技,2015(6)
[13] 匡猛,王研琴,周大云,等.棉花DUS测试标准品种的SSR指纹数据库构建[J].棉花学报,2015,27(1):46-52
[14] 陆光远,吴晓明,张东晓,等.SSR标记分析国家油菜区试品种的特异性和一致性[J].中国农业科学,2008,41(1):32-42
[15] 王艳平,沈奇,张继红,等.大麦DUS测试标准品种的遗传多样性分析及指纹图谱的构建[J].麦类作物学报,2013,33(2):273-278
[16] 程本义,施勇峰,沈伟峰,等.水稻品种DNA指纹检测技术体系及其应用[J].杂交水稻,2008,23(1):54-59
[17] 张金渝,张建华,杨晓洪,等.玉米DUS测试标准品种的SSR分子指纹图谱的构建[J].玉米科学,2006,14(4):47-52
[18] 王缨,陈建民,曾玉荣,等.我国植物新品种博湖对育种科研的影响及对策[J].福建农业学报,2015,30(4):411-416
[19] Sreenivasan T.V., Ahloowalia B.S., Heinz D.J..Cytogenetics. In:Heinz D.J.(ed.) sugarcane improvement through breeding[M]. Amsterdam:Elsevier
[20] 刘新龙,毛钧,陆鑫,等.甘蔗SSR和AFLP分子遗传连锁图谱构建.作物学报.2010,36(1):177-183)
[21] Pan Y.-B., Cordeiro G.M., Richard E.P. Jr., et al.. Molecuar genotyping of sugarcane clones with microsatellite DNA markers[J]. Maydica,2003a,48:319-329.
[22] Pan, Y.-B., Miller J. D., Schnell R. J., et al.. Application of microsatellite and RAPD fingerprints in the Florida sugarcane variety program[J]. Sugar Cane International, March/April 2003b, 19-28
[23] Pan Y.-B. Highly polymorphic microsatellite DNA markers for sugarcane germplasm evaluation and variety identity testing[J]. Sugar Technology, 2006, 8(4):246-256
[24] Qi Y.-W, Pan Y.-B., Lao F.-Y., et al.. Genetic structure and diversity of parental cultivars involved in China mainland sugarcane breeding programs as inferred from DNA microsatellites[J]. Journal of Integrative Agriculture,2012,11(11):1794-1803
[25] Chen P.H., Pan Y.-B., Chen R.-K., et al.. SSR marker-based analysis of genetic relatedness among sugarcane cultivars (Saccharum spp. hybrids) from breeding programs in China and other countries[J]. Sugar Tech,2009,11(4):347-354
[26] Tew T.L. and Pan Y.-B. Microsatellite (Simple Sequence Repeat) marker-based paternity analysis of a seven-parent sugarcane polycross[J]. Crop Science, 2010,50:1401-1408
[27] 梁俊,Pan Yong-Bao,李杨瑞,等.甘蔗属不同种及优良甘蔗栽培品种的SSR标记遗传多样性分析[J].广西植物,2010,30(5):594-600
[28] 郑益凤.甘蔗亲本遗传多样性的SSR荧光标记分析与指纹图谱构建[D].福建农林大学硕士学位论文.2009
[29] Hameed U., Pan Y.-B, Muhammad K., et al.. Use of simple sequence repeat markers for DNA fingerprinting and diversity analysis of sugarcane (Saccharum spp) cultivars resistant and susceptible to red rot[J]. Genetics and Molecular Research, 2012, 11(2):1195-1204
[30] 刘新龙,马丽,陈学宽,等.云南甘蔗自育品种DNA指纹身份证构建[J].作物学报,2010,36(2):202-210
[31] Chandra A., Grisham M.P., Pan Y.-B.. Allelic divergence and cultivar-specific SSR alleles revealed by capillary electrophoresis using fluorescence-labeled SSR markers in sugarcane[J]. Genome, 2014,57(6): 363-372
[32] Pan Y.-B, Burner D.M., Legendre B.L.. An assessment of the phylogenetic relationship among sugarcane and related taxa based on the nucleotide sequence of 5S rRNA intergenic spacers[J]. Genetica, 2000,108:285-295
[33] Rohlf F.J.. NTSYS-pc: numerical taxonomy and multivariate analysis system Version 2.1[J]. Exeter publishing Setauket, New York, 2000
[34] Milbourne, D., Meyer R., Bradshaw J. E., et al.. Comparison of PCR- based marker system the analysis of genetic relationships in cultivated potato[J]. Mol. Breed,1997, 3:127-136
[35] Pan Y.-B., Scheffler B.S., Richard E.P., et al.. High-throughput genotyping of commercial sugarcane clones with microsatellite (SSR) DNA fingerprints[J]. Sugar Tech, 2007, 9(4):176-181
[36] Price S.. Interspecific hybridization in sugarcane breeding[J]. Proc.Int.Soc. Sugar Cane Technol. 1965, 12:1021-1026
[37] Roach B.T. Nobilisation of sugarcane[J]. Proc Int Soc Sugar Cane Technol. 1972, 14:206-216
[38] D’Hont A., Glaszmann J.C.. Sugarcane genomoe analysis with molecular markers, a first decade of research[J]. Proc Int Soc Sugarcane Technol. 2001, 24:556-559
[39] 姚春雪,王先宏,何丽莲,等.甘蔗与蔗茅杂交不同世代的SSR指纹图谱构建[J].分子植物育种,2011,9(3):381-389
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