为研究不同浓度硝酸盐、铵盐和磷酸盐对斯氏角孔珊瑚、二异角孔珊瑚和澄黄滨珊瑚CAT和SOD活性的影响，设置硝酸盐、铵盐和磷酸盐高、中、低3种试验浓度对斯氏角孔珊瑚、二异角孔珊瑚和澄黄滨珊瑚分别进行8周的养殖观察。结果表明：斯氏角孔珊瑚以中等硝酸盐和铵盐浓度组CAT活性最高，高浓度磷酸盐组CAT活性高，以高浓度硝酸盐和磷酸盐组SOD活性最高，中等浓度铵盐组SOD活性最高；二异角孔珊瑚以中等浓度硝酸盐、铵盐和磷酸盐CAT活性最高，以中等浓度硝酸盐、铵盐组SOD活性最高，以高浓度磷酸盐组SOD活性最高；澄黄滨珊瑚以对照组CAT和SOD活性最高，说明澄黄滨珊瑚对营养盐的耐受性差；珊瑚CAT和SOD活性均出现峰值，与其他时间点比较差异显著(P<0.05)，是珊瑚在营养盐胁迫下从适应到中毒反应的临界值，说明珊瑚是可以间接评价海洋环境营养盐富集程度的指示生物。
Abstract
To study the effects of nitrogen and phosphorus on the activities of catalase (CAT) and superoxide dismutase (SOD) in Goniopora stutchburyi, Goniopora duofasciata and Porites lutea, nitrate, ammonium and phosphate were used to conduct the experiments. And the concentrations of the above nutrients were set to be in low, medium and high level, respectively, and the cultivation of the corals lasted for 8 weeks. The results indicated that activities of CAT in G. stutchburyi exposed to medium concentration of nitrate and ammonium , activities of SOD exposed to high concentrations of nitrate and phosphate and to medium concentrations of ammonium reached the highest; Activities of CAT in G. duofasciata exposed to medium concentration of nitrate, ammonium and phosphate, activities of SOD exposed to medium concentrations of nitrate and ammonium and to high concentrations of phosphate reached the highest; Activities of CAT and SOD in P. Lutea with control group reached the highest,it showed poor tolerance to nutrients; CAT and SOD in Goniopora stutchburyi, Goniopora duofasciata and Porites lutea had peaks, and there were significant differences in enzymatic activity among different time points(P<0.05).It was the critical value from adaptation to toxic reaction under stress of nutrients, it illustrated that corals could be the indirectly indicator organism for evaluating the nutrient enrichment degree of marine environment.
关键词
氮;磷;过氧化氢酶;超氧化物歧化酶;珊瑚
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Key words
nitrogen ;phosphorus ; catalase;superoxide dismutase; corals
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