为探讨酚酸类物质与牡丹试管苗生根的关系,以生根率不同的牡丹品种‘太平红’、 ‘乌龙捧盛’和‘凤丹白’为材料,利用液相色谱-质谱联用(LC-MS)方法对试管苗生根过程中6种酚类物质成分定性定量分析,研究其在牡丹试管苗生根过程中的动态变化规律。结果表明：试管苗在生根过程中,3个品种体内的6种酚酸含量均发生了动态性变化,但不同品种变化规律不同。生根率越高的品种,其体内的芍药苷、苯甲酸、4-羟基苯甲酸含量越高,而没食子酸甲酯和莽草酸含量则越低,没食子酸含量在3个品种中未能表现出类似的规律。本研究结果认为芍药苷、苯甲酸、4-羟基苯甲酸、没食子酸甲酯、莽草酸5种酚酸可能与牡丹试管苗生根密切相关,而没食子酸可能与牡丹试管苗生根关系不大。
Abstract
The dynamic changes of six phenolic acids contents were evaluated by using high performance liquid chromatography-mass spectrometry during vitro rooting process of three cultivars (‘Wu Long Peng Sheng’, ‘Tai Ping Hong’, ‘Feng Dan Bai’ ) in order to research the relationship between phenolic acids and the occurrence of adventitious roots in tree peony (Paeonia suffruticosa Andr.). Results showed that six phenolic acids contents varied with time in different amplitude during the vitro rooting process. However, they were difference in the dynamic changes among the three tree peony cultivars. Paeoniflorin, benzoic acid and 4-hydroxy benzoic acid contents were higher in easy-to-root ‘Tai Ping Hong’ cultivar than in difficult-to-root ‘Feng Dan Bai’ and ‘Wu Long Peng Sheng’ cultivars, while methyl gallate and shikimic acid contents were lower in ‘Tai Ping Hong’ than in ‘Feng Dan Bai’ and ‘Wu Long Peng Sheng’. However, no clear conclusions could be drawn for gallic acid content in correlation to the rooting ability of the three tree peony cultivars. The results held that paeoniflorin, benzoic acid, 4-hydroxy benzoic acid, methyl gallate and shikimic acid were closely related to the rooting of tree peony, the influence of gallic acid in rooting was little.
关键词
牡丹;生根;LC-MS;酚酸;动态变化
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Key words
Tree peony; Rooting; LC-MS; Phenolic acids; Dynamic changes
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