葡萄热激转录因子分类及其相关SSR 标记分析

杨海波,张华丽,梁芳,秦贺兰,王茂良,辛海波

中国农学通报. 2016, 32(10): 157-161

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中国农学通报 ›› 2016, Vol. 32 ›› Issue (10) : 157-161. DOI: 10.11924/j.issn.1000-6850.casb15070032
生物技术科学

葡萄热激转录因子分类及其相关SSR 标记分析

  • 杨海波,张华丽,梁芳,秦贺兰,王茂良,辛海波
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Classification of HSFs and Analysis of Their SSR Markers in Grape

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摘要

热害是目前葡萄设施生产中存在的主要问题之一。作为耐热性的主要调控因子,HSF的分析及其基因序列中分子标记研究对葡萄耐热相关育种具有重要意义。通过植物基因组数据库Phytozome(http://phyto5.phytozome.net/)下载19 条葡萄热激转录因子(heat shock factor,HSF)蛋白序列,并对各HSF的氨基酸数目、分子量和等点电进行了预测。以拟南芥HSF为参照,经过多序列比对和进化树分析,对葡萄HSF进行序列分析和分类,发现葡萄基因组中HSF有10 个A类、7 个B类和2 个C类。同时,分析了19 条HSF 基因序列中SSR(simple sequence repeats)位点,并设计特异引物,以‘赤霞珠’基因组DNA为模板,通过PCR(polymerase chain reaction)对引物有效性进行验证。结果表明,葡萄基因组内包括3 类HSF因子编码基因,它们的序列上存在SSR位点,可用于分子标记的开发。研究结果为葡萄耐热相关分子辅助育种提供了参考。

Abstract

Heat stress restricts grape production, especially in facility cultivation. Analysis of SSR markers and classification of HSFs which are major regulators in heat tolerance could be important in grape heat tolerance breeding. In this study, 19 HSF sequences of Vitis vinifera were downloaded from Phytozome. Based on these sequences of VvHSFs, their lengths, molecular weights and isoelectric points were predicted. Furthermore, multiple alignment and phylogenetic analysis of these 19 HSFs were carried out with AtHSFs as reference sequences. 10 HSFAs, 7 HSFBs and 2 HSFCs were found in grape genome. According to SSR sites in VvHSF, the specific primers were designed for the PCR using‘Cabernet Sauvignon’as template. These data showed that grape genome harbored 3 classes of HSFs containing SSR sites which could be used for designing molecular markers. This work would be a basis for molecular marker assistant breeding in grape thermotolerance.

关键词

葡萄;热激转录因子;SSR;分子标记

Key words

grape; heat shock factor; SSR; molecular mar

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杨海波,张华丽,梁芳,秦贺兰,王茂良,辛海波. 葡萄热激转录因子分类及其相关SSR 标记分析. 中国农学通报. 2016, 32(10): 157-161 https://doi.org/10.11924/j.issn.1000-6850.casb15070032
Classification of HSFs and Analysis of Their SSR Markers in Grape. Chinese Agricultural Science Bulletin. 2016, 32(10): 157-161 https://doi.org/10.11924/j.issn.1000-6850.casb15070032

参考文献

[1] 杨治元,南方大棚葡萄调查,中国南方果树,2012,41(5):107-108
[2] Von Koskull-D?ring P, Scharf KD, Nover L. 2007. The diversity of plant heat stress transcription factors [J]. Trends Plant Science, 12:452 – 457
[3]Liu HC, Charng YY. 2012. Acquired thermotolerance independent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response[J]. Plant Signal Behav 7(5):547–550.
[4] Vierling E.1991. The roles of heat shock proteins in plants[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 42:579 – 620
[5] Sun ming-xiao, Zhao ting, Gao ang, Jia xu, et al.. 2011. Overview of pharmacological research on Aquilegia L[J]. Journal of Liaoning university of TCM, 13(4):83-84
[6] 程小毛,黄晓霞 SSR标记开发及其在植物中的应用[J]. 中国农学通报 2011,27(5):304-307
[7] 张运兴,李卫国,申亚琳,杨伟鑫 菊花EST-SSR标记的开发与应用[J].武汉大学学报(理学版)2013,59(4):357-362
[8] 赵勇,杨凯,Akbar Ali Cheema,翁跃进 利用水稻功能基因SSR标记鉴定水稻种质资源[J]. 中国农业科学 2002,35(4):349-353
[9] Nover L, Scharf K D, Gagliardi D, et al. 1996. The Hsf world: classification and properties ofplant heat stress transcription factors [J]. Cell Stress Chaperones, 1: 215 – 223
[10] Nover L, Bharti K, Doring P, et al.. 2001. Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need? [J] Cell Stress Chaperones, 6: 177 – 189
[11] Miller G, Mittler R, 2006. Could Heat Shock Transcription Factors function as hydrogen peroxide sensors in plant, Annals of Botany, 98:279 – 288
[12] Guo J K, Wu J, Ji Q, Wang C, et al. 2008. Genome-wide analysis of heat shock transcription factor families in Rice and Arabidopsis [J]. Journal of Genetics Genomics, 35: 105 – 118
[13] Mishra SK, Tripp J, Winkellaus S, et al. 2002. In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of themotolerance in tomato [J]. Genes Devepment, 16: 1555 – 1567
[14] Bharti K, Koskull-Doring PV, Bharti S, Kumar P, et al. 2004. Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with the plant CREB binding protein ortholog HAC1 [J]. Plant Cell, 16: 1521 – 1535
[15] Baniwal SK, Chan KY, Scharf K-D, et al. 2007. Role of heat stress transcription factor HsfA5as specific repressor of HsfA4 [J]. Journal of Biological Chemistry, 282: 3605 – 3613
[16] Liu HC, Liao HT, Charng YY. 2011. The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis [J]. Plant Cell Environment 34(5):738–751.
[17] Liu HC, Charng YY. 2012. Acquired thermotolerance independent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response [J]. Plant Signal Behavior 7(5):547–550.
[18] 陈培琴,郁松林,詹妍妮,等.茉莉酸对葡萄幼苗耐热性的影响 [J]. 石河子大学学报(自然科学版)2006,24(1):87-91
[19] 王娟,陶永焕,宋尚伟.葡萄EST-SSR引物的开发及部分种质聚类分析 [J].华北农学报,2014,29(2):121-126
[20] 吴子龙,王军,沈有杰,等. 8个山葡萄及山欧杂种葡萄品种的SSR分析 [J]. 植物遗传资源学报 2008,9(1):105-109
[21] 张淑静,杨敏生,梁海永,等. 葡萄SSR反应体系的优化 [J]. 河北林果研究. 2008,23(3):281-286
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