研究不同浓度肌苷对绿豆种子萌发及幼苗生长的影响，为其应用于植物生长调节提供参考。通过室内水培试验，分别设定100~500mg/L五组不同浓度的肌苷处理，测定绿豆种子发芽和植株生长的相关指标。与对照相比，不同浓度肌苷浸种均促进了绿豆种子的萌发和生长，绿豆的发芽势、发芽率、根长、叶长、叶宽、鲜重和干重有不同程度提高，根冠比降低。不同浓度处理中，以400mg/L处理的幼苗发芽势和发芽率最高，分别为95.8%和97.9%；以300mg/L处理鲜重、总干重和根干重最高，播种8、10天后以200mg/L和400mg/L处理的幼苗根长、叶长、叶宽最高。利用肌苷浸种可促进绿豆种子萌发和幼苗生长，且浓度为200~400mg/L肌苷更能够在不同程度上有益于苗期生长并提高产量。
Abstract
The present experiment was conducted to study the influences of different concentrations of inosine on seed germination and seedling growth of mung bean plant, in order to provide references for its application in plant growth regulator. By using indoor hydroponic culture, five different concentrations of inosine (100- 500 mg/L) were designed to determine relevant indicators on seed germination and seedling growth of mung bean. Compared with the control, soaking in all different inosine concentrations could promote the seed germination and seedling growth of mung bean seeds, and improve the seed germination potential and rate, root length, leaf length and width, fresh weight and dry weight in various degrees, while root shoot ratio decreased. Among all inosine treatments, the seed germination and rate of 400 mg/L treatment were the highest, which were 95.8% and 97.9% , respectively. And the fresh weight, dry weight and root dry weight of 300 mg/L treatment were the maximum. Eight and ten days after seeding, seedling root length, leaf length and width of seedling of 200 mg/L and 400 mg/L treatments were also the highest. The use of inosine soaking could promote the seed germination and seedling growth of mung bean, and the inosine concentration of 200-400 mg/L can be more beneficial to the seedling growth and increase the output in varying degrees.
关键词
肌苷;绿豆;种子萌发;幼苗生长
{{custom_keyword}} /
Key words
inosine; mung bean; seed germination; seedling growth
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]宋勇波,蔡显鹏,储炬,等.肌苷合成关键酶活性与肌苷积累之间的关系[J].微生物学报,2003,43(3):361-365.
[2]林容轩,郑莲英,李学明.反相高效液相色谱法测定肌苷发酵和提取液中肌苷和次黄嘌呤的含量[J].科技通报,1991,7(4):207-210.
[3]吕东坡,吕广磊,刘芳芳,等.肌苷发酵条件优化的研究[J].中国食品添加剂,2008, 6(2):161-166.
[4]张克旭,陈 宁,孙振环,等.肌昔高产菌的转化育种及均匀设计在发酵条件试验中的应用[J].食品与发酵工业,1994,1:33-38.
[5]杨尚彤,肖安风,倪 辉,等.枯草芽孢杆菌JMUKC2产肌苷摇瓶发酵培养基的优化[J].食品科学,2012,33(11):167-171.
[6]宋勇波,蔡显鹏,储 炬,等.肌苷合成关键酶活性与肌苷积累之间的关系[J].微生物学报,2003,43(3):361-365.
[7]郭元昕,陈双喜,刘新星,等.不同有机氮源对肌苷合成的影响[J].华东理工大学学报(自然科学版)[J].32(11):1289-1292.
[8]王美华,黄丽英,黄丽萍,等.肌苷分子印迹聚合物的合成及其吸附性能研究[J].药物分析杂志,2013,33(4):566-571.
[9]王丽侠,程须珍,王素华,等.绿豆种质资源、育种及遗传研究进展[J].中国农业科学, 2009,42(5):1519-1527.
[10]陈强培,郭楚玲,廖长君,等.绿肥植物绿豆去除土壤中芘的实验研究[J].农业环境科学学报,2013,32(6):1172-1177.
[11]周 清,李保同,陈洁琼,等.大蒜素对3种蔬菜种子发芽和幼苗生长的影响[J].中国农学通报 2014,30(13):207-213.
[12]纪秀娥,史留功,胡春红,等.油菜素内酯对小麦、玉米种子萌发的影响[J].江苏农业科学,2014,42(9):88-89.
[13]仕相林,孙亚男,王家麟,等.大豆叶片性状QTL的定位及Meta分析[J].作物学报,2012,38(2): 256-263.
[14]陈晓远,高志红,罗远培.植物根冠关系[J].植物生理学通讯,2005,41(5):555-561.
[15]罗 珊,康玉凡,夏祖灵.种子萌发及幼苗生长的调节效应研究进展[J].中国农学通报.2009,25(02):28-32.
[16]于秋良,杨志贤,李忠芳,等.pH值对绿豆种子萌发和幼苗生长的影响[J].南方农业学报.2012,43(10):1466-1469.
[17]王艳哲,刘秀位,孙宏勇,等.水氮调控对冬小麦根冠比和水分利用效率的影响研究. 中国生态农业学报.2013,21(3):282-289.
[18] LIOERT F, CASANOVAS C, PENUELAS J. Seedling survival of Mediterranean shrub land species in relation to root: shoot ratio, seed size and water and nitrogen use [J]. Functional Ecology, 1999,13(2):210-216.
[19]孔祥生,张妙霞.油菜素内酯和多效唑对玉米种子萌发及幼苗生长的影响[J].中国农学通报,1998,14(2):21-23.
[20]张琳.油菜素内酯的生理效应及发展前景[J].北方园艺,2011(20):188-191.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
PDF(626 KB)
