一种脉孢霉的分离鉴定及拮抗菌筛选

王珊珊,杨佳玥,袁雪,张国庆,陈青君,田昆利

中国农学通报. 2015, 31(21): 157-161

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中国农学通报 ›› 2015, Vol. 31 ›› Issue (21) : 157-161. DOI: 10.11924/j.issn.1000-6850.casb15010209
生物技术科学

一种脉孢霉的分离鉴定及拮抗菌筛选

  • 王珊珊,杨佳玥,袁雪,张国庆,陈青君,田昆利
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Isolation, Identification, and Screening of Antagonistic Bacteria of A Neurospora sp. Strain

  • 王珊珊,杨佳玥,,, and
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摘要

对黑木耳栽培中的污染杂菌进行分离鉴定及拮抗菌筛选，为进一步研究和治理提供理论依据。采用组织分离法获得纯培养菌株，基于ITS鉴定其分类学地位，采用平板对峙法筛选生防放线菌。获得纯培养菌株，编号CP-1。ITS序列扩增，得到一条571bp的序列(GenBankNo.KP027411)；与好食脉孢霉和粗糙脉孢霉的相似度分别为99%和98%；结合形态学特征，CP-1与好食脉孢霉的相似性更高。生防链霉菌BUAS13-03菌株抗性最佳，抑菌圈直径为2.8±0.3cm。确定菌株CP-1为好食脉孢霉；生防菌BUAS13-03对CP-1的拮抗效果较好。本研究对黑木耳好食脉孢霉的生物防治提供理论基础和试验依据。

Abstract

The aim was to identify the contaminative fungus from the culture media of mushroom Auricularia auricula- judae, screen the antagonistic bacteria and provide theoretical basis for further applications. Pure culture strain was obtained using tissue isolation method. Phylogenetic identification was based on ITS sequencing. The antagonist study was conducted with plate confrontation method. A strain numbered CP-1 was obtained. Partial ITS sequence was 571 bp (GenBank No. KP027411). It shared highest sequence similarity towards Neurospora sitophila and N. crassa with the value of 99% and 98%, respectively. CP-1 was identified as N. sitophila based on molecular and morphological identification. The Streptomyces strain BUAS13- 03 demonstrated the highest antifungal activity with the inhibition zone of 2.8 ± 0.3 cm. The strain CP- 1 was identified as N. sitophila. The Streptomyces strain BUAS13-03 manifested considerably high inhibitory activity towards CP-1. The present study is helpful for the further applications of biological control for N. sitophila.

关键词

黑木耳； 好食脉孢霉； ITS； 拮抗效果

Key words

Auricularia auricula-judae; Neurospora sitophila; ITS; antagonistic effect

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王珊珊,杨佳玥,袁雪,张国庆,陈青君,田昆利. 一种脉孢霉的分离鉴定及拮抗菌筛选. 中国农学通报. 2015, 31(21): 157-161 https://doi.org/10.11924/j.issn.1000-6850.casb15010209
王珊珊,杨佳玥,,, and. Isolation, Identification, and Screening of Antagonistic Bacteria of A Neurospora sp. Strain. Chinese Agricultural Science Bulletin. 2015, 31(21): 157-161 https://doi.org/10.11924/j.issn.1000-6850.casb15010209

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