为了研究甘蓝型油菜防御素基因的结构和功能，根据白菜防御素基因序列设计引物，采用RT-PCR方法从甘蓝型油菜中克隆到4个防御素基因，并对其序列进行了生物信息学分析。结果表明，甘蓝型油菜防御素基因cDNA全长325~335 bp，包含231~243 bp的开放阅读框，编码76~80个氨基酸；防御素基因在长期进化过程中产生了显著的差异，但防御素含有的8个保守半胱氨酸残基均稳定存在；Bndef1、Bndef2、Bndef3与萝卜Rs-AFP1、诸葛菜Omdef亲缘关系较近，Bndef4与豇豆Vudef亲缘关系较近；4个防御素蛋白均为稳定蛋白，具有信号肽结构，可能为分泌蛋白。
Abstract
The aims were to investigate the structure and function of Brassica rapa defensin genes. According to the sequence of Brassica rapa defensin genes, 4 Brassica napus defensin genes were cloned by polymerase chain reaction (PCR) and predicted by bioinformatic methods. The results showed that the cDNA sequence was 325-335 bp, and the open reading frame (ORF) was 231-243 bp which could encode a polypeptide of 78-80 amino acids. Brassica napus defensin genes resulted in significant differences in the long evolutionary process, resulting in significant differences, but the 6-8 conservative cysteine residues are stable. Phylogenetic tree showed that Bndef1, Bndef2, Bndef3 had close genetic relationship with Raphanus sativus Rs-AFP1 and Orychophragmus violaceus Omdef, Bndef4 had close genetic relationship with Vigna unguiculata Vudef. The Brassica napus defensin genes defensin proteins were stable protein with a signal peptide structure, which might be secreted protein.
关键词
甘蓝型油菜；植物防御素；克隆；生物信息学分析
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Key words
Brassica napus; plant defensin; gene cloning; bioinformatics analysis
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