为了明确引起北京地区草莓病毒病的病毒种类和病毒序列的分子变异特点，从北京地区表现畸形症状的草莓叶片中提取总RNA，利用RT-PCR技术扩增得到草莓轻型黄边病毒(Strawberry mild yellow edge virus，SMYEV)的含有外壳蛋白(CP)基因的729 nt特异性核苷酸片段。经序列测定和分析可知，北京地区存在2种不同的SMYEV分离物（BJ1和BJ2），两者间的核苷酸序列同源性仅为82.72%~82.96%。将北京分离物与分别来自德国、美国、智利、捷克、澳大利亚、比利时、意大利、韩国和中国沈阳等国家或地区的不同分离物的CP基因序列进行分析比较。结果显示，33个分离物可以分为四大组群，BJI和BJ2分别归属不同组群，BJ1与19个其他国家的分离物亲缘关系较近，BJ2与中国沈阳的2个分离物亲缘关系较近。BJ1和BJ2与其他分离物的CP基因核苷酸序列同源性分别为81.10%~98.35%和82.03%~98.63%，氨基酸序列同源性介于82.17%~99.17%和90.91%~94.63%。
Abstract
Strawberry mild yellow edge virus (SMYEV) were detected from naturally infected strawberry plants with malformation symptoms from fields based on the study to determine the main pathogen of strawberry viral diseases in Beijing. The expected fragments of about 729 nt include whole CP gene of SMYEV were amplified by RT-PCR and were sequenced by PCR products directly to analyze the molecular variance. Sequence analysis showed 2 different isolates of SMYEV named BJ1 and BJ2 were obtained and the nucleotide identity between them ranged from 82.72% to 82.96%. The sequence of CP gene of different isolates from Beijing, Germany, USA, Czech Republic, Australia, Belgium, Italy, Korea and Shenyang were analyzed. The results showed 33 isolates were divided into 4 groups in which BJ1 and BJ2 belonged to different groups. The identities of nucleotide and deduced amino acid sequences of CP gene between BJ1 and other isolates ranged from 81.10% to 98.35% and 82.17% to 99.17%, and those identities between BJ2 and other isolates ranged from 82.03% to 98.63% and 90.91% to 94.63% respectively.
关键词
草莓轻型黄边病毒；RT-PCR；CP基因；序列分析
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Key words
Strawberry mild yellow edge virus; RT-PCR; CP gene; sequence analysis
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