为了提高蚕豆花左旋多巴的提取得率，采用加热回流提取法优化蚕豆花左旋多巴提取工艺。通过单因素和正交试验设计，考察液料比、溶剂pH、提取时间对蚕豆花左旋多巴提取得率的影响，确定最佳提取工艺条件为：液料比20:1，溶剂pH 4.5，提取时间2 h，在此条件下左旋多巴提取得率为3.67%，相比冷浸法提高了3.67%。本工艺中使用乙醇来调节溶剂的pH值，可以抑制水溶性杂质的析出，经HPLC谱图分析，提取物中杂质组分较少，且左旋多巴与杂质组分分离较好，这有利于左旋多巴进一步的纯化。本研究可为从蚕豆花中工业化提取左旋多巴提供技术依据。
Abstract
In order to improve the extraction rate of levodopa from broad bean flowers, the extraction process was optimized by heating reflux extraction. Through single factor and orthogonal test design, the effects of the ratio of liquid to material, solvent pH and extraction time on the extraction yield of levodopa from broad bean flowers were investigated. The optimum extraction conditions were determined as follow: the ratio of liquid to material 20:1, solvent pH 4.5, extraction time 2 h. Under the optimized conditions, the extraction rate of levodopa was 3.67%, which was 3.67% higher than cold maceration. The ethanol was used to adjust the pH value of solvent in the process, which could inhibit the precipitation of the water soluble impurities. The impurity components were less in the extract and the separation of levodopa and impurities was good by HPLC, so it’s conducive to the further purification of levodopa. The study can provide a technical basis for the industrial extraction of levodopa from broad bean flowers.
关键词
蚕豆花；左旋多巴；加热提取；正交试验
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Key words
broad bean flowers; levodopa; heating extraction; orthogonal test
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脚注
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