Cloning and Bioinformatic Analysis of CBF1 Gene in Amygdalus communis
Zhang Liang1, Zhang Zhijun2, Li Jiang3, Dai Peihong1, Tian Jia3, Luo Shuping1
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(1College of Agronomy, Xinjiang Agricultural University, Urumqi 830052; 2The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an Zhejiang 311300; 3College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052)
The AcCBF1 gene was cloned and analyzed in order to identify the role of CBF transcription factors in plant resistance of Amygdalus communis. The CBF1 homologue gene named YZ- AcCBF1 (Genbank Accession No. KJ818900) was isolated from‘Yingzui’(Amygdalus communis) with the technology and method of PCR. The bioinformatics analysis showed that the open reading frame was 729 bp in full- length and encodedaprotein of 242 amino acids. The molecular formula was C1195H1886N346O368S13, and its relative molecular mass was approximately 27.405 kDa and isoelectric point was 7.69. This protein was an instable protein whose deduced half-life was 30 h. Its fat factor was 63.72 and the average of hydrophobic was -0.646. Subcellular localization analysis showed that AcCBF1 protein was localized in the nucleus, it was not a secreted protein but the hydrophilic protein and the transmembrane domain did not exist. Phylogenetic analysis revealed that AcCBF1 encode protein showed the highest similarity with Prunus persica (AGS13699). The prediction of secondary structure of protein indicated that the encoded protein mainly contain 26.86% ofα- helices and 58.68% of the random coil. Three- dimensional structure predicted that AcCBF1 protein confirmed the characteristics of AP2 domain model.
Zhang Liang1, Zhang Zhijun2, Li Jiang3, Dai Peihong1, Tian Jia3, Luo Shuping1.
Cloning and Bioinformatic Analysis of CBF1 Gene in Amygdalus communis. Chinese Agricultural Science Bulletin. 2014, 30(34): 16-23 https://doi.org/10.11924/j.issn.1000-6850.2014-1877
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参考文献
[1] Levitt J. Responses of plants to environmental stresses: chilling, freezing, and high temperature stresses[M].New York: Academic Press,1980:698.
[2] Thomashow M F. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms[J]. Annu Rev Plant Phys,1999,50:571-599.
[3] Nakashima K, Yamaguchi-Shinozaki K. Regions involved inosmotic stress-responsive and cold stress-responsive geneexpression in plants[J].Physiol Plant,2006,126:62-71.
[4] Yamaguchi-Shinozaki K, Shinozaki K. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses[J]. Annu Rev Plant Biol,2006,57:781-803.
[5] Medina J, Catalá R, Salinas J. The CBFs: three Arabidopsistranscription factors to cold acclimate[J]. Plant Sci,2011,180:3-11.
[6] Stockinger E J, Gilmour S J, Thomashow M F. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit[J]. Proc Natl Acad Sci USA,1997,94:1035-1040.
[7] Liu Q, Kasuga M, Sakuma Y, et al. Two transcription factors,DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought and low temperature responsive gene expression, respectively, inArabidopsis[J]. Plant Cell,1998,10:1391-1406.
[8] 李疆,胡芳名,张智俊,等.扁桃主要生物学特性的观测[J].经济林研究,2003,21(3):39-40.
[9] 成键红,侯平,李疆,等.巴旦杏的产业发展及其研究进展[J].干旱区研究,2000,17(1):32-38.
[10] Nakano T K, Fujimura Suzuki T, Shinshi H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice[J]. Plant Physiol,2006,140:411-432.
[11] Dubouzet J G, Sakuma Y, Kasuga M, et al. OsDREB genes in rice, Oryza sativa L, encode transcription activators that function in drought, high-salt and cold-responsive gene expression[J]. The Plant Journal,2003,33:751-763.
[12] Francia E, Rizza F, Cattivalli L, et al. Two Loci on chromosome 5H determine low temperature tolerance in a ‘Nure’ (winter)× ‘Tremois’ (spring) barley map[J]. Theoretical and Applied Genetics,2004,108:670-680.
[13] Shen Y G, Zhong W K, He S J, et al. An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress[J]. Theoretical and Applied Genetics,2003,106:923-930.
[14] Artus N N, Uemura M, Steponkus P L. Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance[J]. Proceedings of the National Academy of Science,1996,93:13404-13409.
[15] Medina J, Bargues M, Terol J, et al. The Arabidopsis CBF genefamily is composed of three genes encoding AP2 domain-containing proteins whose expression Is regulated by low temperature but not by abscisic acid or dehydration[J]. Plant Physiol,1999,119(2):463-469.
[16] Gilmour S J, Zarka D G, Stockinger E J, et al. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptionalactivators as an early step in cold-induced COR gene expression[J]. Plant J,1998,16(4):433-442.
[17] 周洲,李永丽.毛白杨转录因子PtCBF5的表达模式分析[J].林业科学,2010,46(4):58-63.
[18] 秦红霞,贾志平,张海超,等.银新杨中与DRE元件结合的转录因子的克隆及鉴定分析[J].生物工程学报,2005,21(6):906-910.
[19] Wang Xunmin, J Dong ie, Liu Yun, et al. A Novel Dehydration-Responsive Element-Binding Protein from Caragana korshinskii Is Involved in the Response to Multiple Abiotic Stresses and Enhances Stress Tolerance in Transgenic Tobacco[J]. Plant Mol Biol Rep,2010,28(4):664-675.