为研究大孔吸附树脂分离枇杷叶科罗索酸的工艺条件及参数，以枇杷叶为原料，通过高效液相色谱法测定枇杷叶科罗索酸浓度，采用乙醇浸提法提取枇杷叶科罗索酸，并用活性炭进行脱色和大孔吸附树脂分离提取液中的科罗索酸。试验结果枇杷叶中科罗索酸提取得量为7.76 mg/g 干粉；枇杷叶提取液脱色条件为：氢氧化钠用量0.2%，活性炭用量1.5%，脱色温度为70℃，脱色时间20 min，枇杷叶提取液的色素去除率达90%，科罗索酸回收率为91.2%；大孔树脂分离静态试验结果表明，NKA9 大孔吸附树脂较适合枇杷叶科罗索酸的分离纯化，静态吸附率为96.3%，解吸率为80.9%。动态试验结果表明，枇杷叶科罗索酸的分离工艺参数为：上样流速为3 BV/h，洗脱剂体积分数为90%乙醇，用量为7 BV，洗脱流速为3 BV/h，获得科罗索酸的纯度为43.12%。
Abstract
The study aims to optimize the purification technology and parameters of macroporous resins to separate corosolic acid from Eriobotrya japonica leaves. Taking Eriobotrya japonica leaves as raw material, the content and purification of corosolic acid were analyzed by high performance liquid chromatography (HPLC), corosolic acid was extracted from loquat leaves by ethanol. Decolorizing was conducted with activated carbon and corosolic acid was separated by macroporous adsorptive resin. Results showed that the yield of corosolic acid was 7.76 mg/g. The optimum condition of decoloring with the activated carbon was: sodium hydroxide dosage of 0.2%, the activated carbon ratio of 1.5%, the temperature of 70℃ and the time of 20 minutes. 90% of pigment was eliminated, 91.2% of CA could be reclaimed. The results of macroporous resin separation static test showed that NKA9 resin was suitable for the separation, the static adsorption rate and desorption rate was 96.3% and 80.9%, respectively. The dynamic test indicated that the corosolic acid was separated by 90% ethanol, 7 BV of dosage and 3 BV/h of flow rate, 43.12% purity of corosolic acid was obtained.
关键词
枇杷叶；科罗索酸；大孔树脂；分离
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Key words
Eriobotrya japonica leaves; corosolic acid; macroporous; separation
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