为探究杀虫剂对香蕉花蓟马科学合理的生物测定方法，采用离心管药膜法、浸叶法和叶管药膜法测定了5种常用杀虫剂原药对香蕉花蓟马二龄若虫的毒力。结果显示：3种生物测定方法对测定结果产生不同的影响，以叶管药膜法的毒力最大，其对5种药剂的毒力(LC50)顺序为：甲维盐(0.36 mg/L)＞毒死蜱(0.90 mg/L)＞吡虫啉(1.64 mg/L)＞高效氯氰菊酯(4.70 mg/L)＞噻嗪酮(12.53 mg/L)，这与离心管药膜法在5种药剂的毒力顺序一致：甲维盐(1.63 mg/L)＞毒死蜱(2.18 mg/L)＞吡虫啉(6.59 mg/L)＞高效氯氰菊酯(7.38 mg/L)＞噻嗪酮(22.06 mg/L)；采用浸叶法对5种药剂的毒力(LC50)顺序为：吡虫啉(2.15 mg/L)＞甲维盐(2.29 mg/L)＞高效氯氰菊酯(7.21 mg/L)＞毒死蜱(7.40 mg/L)＞噻嗪酮(14.95 mg/L)。上述3种生物测定方法都可以建立并应用于香蕉花蓟马的生物测定，离心管药膜法更适合触杀毒力测定。
Abstract
In an effort to test scientific and reasonable bioassay of Thrips hawaiiensis, laboratory studies were conducted on the effect of three bioassay methods including centrifuge tube residual bioassay, leaf-dip bioassay and leaf-tube residual bioassay on toxicity of five original insecticides to against larvae of T. hawaiiensis. It was revealed that toxicity results of the 5 insecticides varied differently among the treatments, leaf-tube residual bioassay performed the highest toxicity and the toxicity (LC50) order was emamectin-benzoate(0.36 mg/L)＞ chlorpyrifos(0.90 mg/L)＞ imidacloprid(1.64 mg/L)＞ betacy-permethrin(4.70 mg/L)＞ buprofezin(12.53 mg/L), which was consistent with centrifuge tube residual bioassay, for emamectin-benzoate(1.63 mg/L)＞ chlorpyrifos(2.18 mg/L)＞ imidacloprid(6.59 mg/L)＞ betacy-permethrin(7.38 mg/L)＞ buprofezin(22.06 mg/L). Whereas toxicity order was imidacloprid (2.15 mg/L)＞ emamectin-benzoate(2.29 mg/L)＞ betacy-permethrin(7.21 mg/L)＞ chlorpyrifos(7.40 mg/L)＞ buprofezin(14.95 mg/L) when leaf-dip bioassay was applied in the research. In summary, present study suggested above three methods would be establish and apply in laboratory bioassay on Thrips hawaiiensis, and centrifuge tube residual bioassay was more suitable for using in contact toxicity bioassay.
关键词
香蕉花蓟马； 生物测定方法； 杀虫剂； 毒力； 害虫治理
{{custom_keyword}} /
Key words
Thrips hawaiiensis; bioassay methods; insecticides; toxicity; pest management
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Murai T. Development and reproductive capacity of Thrips hawaiiensis (Thysanoptera: Thripidae) and its potential as a major pest[J]. Bulletin of Entomological Research,2001 91:193-198.
[2] 曾鑫年,林进添.黄胸蓟马对香蕉的危害及其防治[J].植物保护, 1998,24(6):15-17.
[3] 林明光,刘福秀,彭正强,等.海南省香蕉作物害虫调查与鉴定[J].西南农业大学学报,2009,22(6):1619-1622.
[4] 蔡云鹏,黄明道,陈新评.香蕉园内花蓟马之发生及其为害[J].中华昆虫,1992,12(4):231-237.
[5] Alfredo R, Anthony M S. Development of a bioassay system for monitoring susceptibility in Thrips tabaci[J]. Pest Manag. Sci,2003, 59(5):553-558.
[6] Bielza P, Quinto V, Contreras J, et al. Resistance to spinosad in the western flower thrips Frankliniella occidentalis in greenhouses of south-eastern Spain[J]. Pest Manag Sci,2007,63:682-687.
[7] 龚佑辉,吴青君,张友军,等.多杀菌素低剂量处理西花蓟马对药剂敏感性的影响[J].植物保护,2010,36(4):138-141.
[8] 陈雪林,孙蓉,杜予州,等.阿维菌素与三种杀虫剂对西花蓟马的联合毒力[J].植物保护,2011,37(5):206-209.
[9] 王健立,李洪刚,郑长英.西花蓟马与烟蓟马药剂敏感性的比较[J].应用昆虫学报,2011,48(3):548-552.
[10] 陈斌,张琦,桂富荣,等.球孢白僵菌不同分离株对西花蓟马的毒力测定[J].生物安全学报,2012,21(1):14-19.
[11] 张安盛,张思聪,李丽莉,等.3种环境友好型药剂对西花蓟马的室内毒力与田间防效[J].植物保护,2012,38(4):175-177.
[12] Dagli F, Tunci. Insecticide resistance in Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) collected from horticulture and cotton in Turkey[J] Aust J Entomol,2007,46(4):320-324.
[13] 王圣印,刘永杰,周仙红,等.环境因子对西花蓟马室内生物测定结果的影响[J].农药学学报,2012,14(2):158-164.
[14] 卢辉,钟义海,刘奎,等.6种杀虫剂对香蕉花蓟马的室内毒力测定[J].中国农学通报,2010,26(6):240-242.
[15] 孟和生.两种生测方法对杀螨剂毒力测定结果的影响比较[J].植物保护,2002,28(3):49-51.
[16] 何玉仙,梁智生,林桂君,等.烟粉虱成虫对烟碱类杀虫剂抗性的生物测定方法[J].福建农林大学学报,2006,35(2):144-146.
[17] 张永强,丁伟,吴静,等.3种杀螨剂生物测定方法的比较研究[J].农药科学与管理,2007,28(7):50-53.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
