分析平欧杂种榛间的遗传关系，为遗传图谱的绘制及优良基因的选育奠定基础。该实验选用21份主栽品种，经DNA提取、SSR标记及NTSYSpc (version 2.11 a)对结果的分析建立遗传聚类图。筛选6对欧榛SSR引物对供试材料进行检测，共检测出71个遗传位点，全部为多态性遗传位点，多态位点百分率为100%。遗传相似系数为0.34~0.82，平均相似系数为0.55，当阈值为0.74时，供试样品可聚类分为4个组。C1、C13和C2聚为一组，即组Ⅰ；C6和C15聚为一组，即组Ⅱ；C7、C9、C14、C16和C10聚为一组，即组Ⅳ；其余样品聚为一组，即组Ⅲ。平欧杂种榛品种间具有丰富的遗传多样性，供试主栽品种间存在着复杂的亲缘关系；构建的技术体系可为其他相关试验提供参考。
Abstract
The analysis of genetic relationship of Ping’ou hybrid hazelnut laid the foundation for the genetic mapping and the breeding of choiceness gene. 21 main cultivars were selected for the experiment. After DNA extraction, SSR molecular marker and NTSYSpc (version 2.11 a) analysis of the results, constructed a dendrogram. 6 primer pairs of Corylus avellana were selected for the detection of the tested materials. 71 genetic loci were detected, which were polymorphic loci in all. The percentage of polymorphic bands was 100%. The SSR-derived genetic similarity was 0.34-0.82, with an average of 0.55. All the samples were divided into 4 groups when the threshold value was 0.74. C1, C13 and C2 constituted the groupⅠ; C6 and C15 constituted the groupⅡ; C7, C9, C14, C16 and C10 constituted the groupⅣ. The others constituted the groupⅢ. There were genetically abundant diversities among Ping’ou hybrid hazelnut. The genetic relationships of the main cultivars were complex. Analysis system established in this study could offer reference for other relative researches.
关键词
平欧杂种榛； SSR； 遗传多样性
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Key words
Ping’ ou hybrid hazelnut; SSR; genetic diversity
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