为分析水稻品种‘连粳7号’对水稻黑条矮缩病的抗性，获得与抗性基因紧密连锁的分子标记，通过田间自然接种和室内人工接种方法，对‘连粳7号’及其与感病品种‘培矮64’衍生来的F2:3群体进行黑条矮缩病抗性鉴定。结果表明，‘连粳7号’发病率最高为12.25%，最低为5.5%。‘连粳7号’的亲本中，仅‘中粳川-2’的抗性与‘连粳7号’相近。另外，‘连粳7号’对灰飞虱不具有抗性，所以‘连粳7号’对黑条矮缩病的抗性是品种本身对病毒的抗性所致。连粳7号/培矮64 F2:3群体中，各个家系表型呈现连续分布，并具有超亲分离特点，说明抗性由多基因控制。最终，通过BSA法获得与抗性位点紧密连锁的标记RM287。综上，‘连粳7号’对黑条矮缩病具有较强抗性，抗性来自亲本‘中粳川-2’，标记RM287将为利用分子标记辅助培育抗黑条矮缩病水稻新品种提供有力工具。
Abstract
To analyze the resistance of‘Lianjing 7’against rice black-streaked dwarf disease, and find the linked marker with the resistant gene, the resistance analysis of‘Lianjing 7’, its parents and the F2:3 population, which derived from a cross between‘Pei’ai 64’, a susceptible variety, and‘Lianjing 7’, a variety with resistance to RBSDS, were evaluated using natural infection methods and artificial inoculation by scoring the disease rating. The result showed‘Lianjing 7’was resistant to RBSDD with the disease rate between 5.5% and 12.25% in all test. In the parents of‘Lianjing 7’, only‘Zhongjingchuan-2’was resistant to RBSDD. The antixenosis and antibiosis score of‘Lianjing 7’was similar to the susceptible variety‘Wuyujing 3’, so the resistance of‘Lianjing 7’was mainly caused by the resistance to the RBSDV. In the Lianjing 7/Pei’ai 64 F2:3 population, the disease rates showed a continuous distribution with transgressive segregation, it indicated the resistance of‘Lianjing 7’was controlled by polygenic. Finally, we got the marker RM287 closely linking with the resistance loci by BSA. To sum up,‘Lianjing 7’was resistant to RBSDD, and it acquired the resistance of‘Zhongjingchuan-2’. The marker RM287 should be useful for breeding new RBSDD-resistant cultivars by marker-assisted selection (MAS).
关键词
水稻；‘连粳7号’； 黑条矮缩病； 抗性分析
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Key words
rice (Oryza sativa L.);‘Lianjing 7’ ; RBSDD; resistance analysis
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参考文献
[1] Milne R G, Lovisolo O. Maize rough dwarf and related viruses[J]. Adv Virus Res,1977(21):267-341.
[2] Azuhata F, Uyeda I, Kimura I, et al. Close similarity between genome structures of rice black- streaked dwarf and maize rough dwarf viruses[J]. J Gen Virol,1993(74):1227-1232.
[3] 李德葆,王拱辰,盛方镜.浙江省水稻病毒病的发生规律和防治[J].植物病理学报,1979,9(2):73-87.
[4] Heng M Z, Yang J, Chen J P, et al. A black-streaked dwarf disease on rice in China is caused by a novel fiji virus[J]. Arch Virol,2008 (153):1893-1898.
[5] Zhang H M, Chen J P, Lei J L, et al. Sequence analysis shows that a dwarfing disease on rice, wheat and maize in China is caused by rice black- steaked dwarf virus[J]. Eur J Plant Pathol,2001,107(5): 563-567.
[6] Wang H D, Chen J P, Wang A G, et al. Studies on the epidemiology and yield losses from rice black-streaked dwarf disease in a recent epidemic in Zhejiang province, China[J]. Plant Pathol,2009(58): 815-825.
[7] 江苏省农业会员会, 水稻黑条矮缩病发生特点及治理对策,2009- 05-11.
[8] 李爱宏,戴正元,季红娟,等.不同基因型水稻种质对黑条矮缩病抗性的初步分析[J].扬州大学学报,2008,29(3):18-22.
[9] 潘存红,李爱宏,陈宗祥,等.水稻黑条矮缩病抗性 QTL分析[J].作物学报,2009,35(12):2213-2217.
[10] 王宝祥,江玲,陈亮明,等.水稻黑条矮缩病抗性资源的筛选和抗性QTL的定位[J].作物学报,2010,36(8):1258-1264.
[11] 方雷,张涛,高云,等.淮北稻区水稻品种适应性及生产力筛选试验研究[J].农业科技通讯,2011(6):56-60.
[12] 周彤,吴丽娟,王英,等.灰飞虱从冷冻病叶获得水稻黑条矮缩病毒方法的研究初报[J].中国水稻科学,2010,24(4):425-428.
[13] Nemoto H, Ishikawa K, Shimura E. The resistance to rice stripe virus and small brown planthopper in rice variety IR50[J]. Breeding Science,1994,44(1):13-18.
[14] Duan C X, Zhang S X, Lei C L, et al. Evaluation of rice germplasm for resistance to the small brown planthopper (Laodelphax striatellus) and analysis of resistance mechanism[J]. Rice Sci,2008, 15(1):36-42.
[15] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Res,1980,819(19):4321- 4325.
[16] Akaji H, Yokozeki Y, Inag akaii, et al. Micron, a microsatellitetarget ingtransposable element in the rice genome[J]. Mol Gen Genomics,2001,266(3):471-480.
[17] Michelmore R W, Paranand I, Kessali R V. Identification of markers linked to disease resistance gene by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations[J]. Proc Natl Acad Sci USA, 1991(88):9829-9832.
[18] Lincoln S, Daly M, Lander E. Constructing genetics maps with Mapmaker/Exp 3.0[R]. Whitehead Institute Technical Report, Cambridge, Massachusetts,1992.
[19] 卢百关,程兆榜,秦德荣,等.江苏水稻主栽和候选品种抗黑条矮缩病鉴定[J].南方农业学报,2011,42(12):1478-1482
[20] Zhang Y X, Wang Q, Jiang L, et al. Fine mapping of qSTV11KAS, a major QTL for rice stripe disease resistance[J]. Theor Appl Genet, 2011(122):1591-1604.
[21] 丁秀兰,江玲,刘世家,等.利用重组自交系群体检测水稻条纹叶枯病抗性基因及 QTL分析[J].遗传学报,2004,31(3):287-292
[22] Wang B X, Jiang L, Zhang Y X, et al. Genetic dissection of the resistance to Rice stripe virus present in the indica rice cultivar‘IR24’ [J]. Genome,2011(54):611-619.
[23] Wang G L, Mackill D J , Bonman J M, et al. RFLP mapping of genes conferring and partial resistance to blast in a durably resistance rice cultivar[J]. Genetics,1994(136):1421-1434.
[24] Yoshimura S, Yoshimura A, Nelson R J, et al. Mapping and combining of bacterial blight resistance genes in rice using molecular markers[J]. Jpn J Breed,1993(43):161-165.
[25] Song W Y, Wang G L, Chen L L, et al. A receptor kinase- like protein encoded by the rice disease resistance gene, Xa21[J]. Science,1995(270):1804-1806.
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