为了开发新的可替代化肥、农药的促植物生长微生物肥料，对从水稻种子中分离得到的1株具有抗氧化活性的内生细菌K12G2进行分类鉴定，检测其促生特性，并通过平板培养和水培试验测定其对水稻幼苗的促生长作用。结合菌株K12G2的形态学、生理生化特征检测及分子生物学鉴定，K12G2为柠檬色短小杆菌(Curtobacterium citreum)；该菌株表现为产IAA、溶磷、固氮及淀粉酶活性的促生特性；在限菌平板培养条件下，菌株K12G2培养液浸种1 h处理比4 h处理后对水稻种子的萌发和生长效果明显，培养液处理的水稻幼苗根长和芽长显著增加；在水培条件下，接种K12G2的水稻幼苗根长显著提高47.08%，生长至三叶期的幼苗比例是对照组的4.5倍。结果表明，菌株K12G2促生作用明显，具有一定的应用前景。
Abstract
In order to exploit new plant growth promoting microbial fertilizer to replace chemical fertilizer and pesticide, an endophytic strain K12G2 which was isolated from rice seeds was identified and its plant growth promoting action was tested by plate cultivation and water planting. Based on morphological, physiological, biochemical and molecular identification, strain K12G2 was belonged to Curtobacterium citreum. Strain K12G2 was capable of IAA production, phosphate solubilization, nitrogen fixing and starch decomposition. Under gnotobiotic culture conditions, rice seeds treated with strain K12G2 for 1 hour showed more effective than seeds treated with 4 hours. Strain K12G2 exhibited significant increases in radicle and plumule elongation of inoculated seeds under gnotobiotic conditions, and root length (47.08%) of rice seedlings by water planting. Furthermore, rate of three-leaf stage seedlings was 4.5 times that of control. This study provided a good experimental strain for further industrial utilization on plant growth promoting.
关键词
内生菌K12G2； 水稻； 促生作用； 柠檬色短小杆菌
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Key words
endophytic strain K12G2; rice; plant growth promoting; Curtobacterium citreum
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