环介导恒温扩增技术(LAMP)及其在植物病毒检测中的应用

秦文韬 王忠跃 张昊

中国农学通报. 2013, 29(21): 170-174

中国农学通报 ›› 2013, Vol. 29 ›› Issue (21) : 170-174. DOI: 10.11924/j.issn.1000-6850.2013-0240
植物保护 农药

环介导恒温扩增技术(LAMP)及其在植物病毒检测中的应用

  • 秦文韬 王忠跃 张昊
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Loop-mediated isothermal amplification(LAMP)and its application in detection of plant virus

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摘要

摘要:环介导恒温扩增技术是一种特异、灵敏、简单的新型核酸扩增技术,目前,该技术已经被广泛应用于疾病诊断、食品安全检测及基因芯片等领域。本文综述了环介导恒温扩增法在植物病毒、类病毒检测中的应用,并讨论了其在应用中出现的问题及解决办法。

Abstract

Abstract:Loop-mediated isothermal amplification(LAMP) is a new nucleic acid amplification technology with the advantage of high specificity, efficiency and rapidity. At present, the technology has been widely used in disease diagnosis, food safety testing and gene chip, etc. This paper reviews application of LAMP in the detection of plant virus and viroid, then discusss the problems and solutions which discovered in the process of application.

关键词

植物类病毒

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Plant viroid

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秦文韬 王忠跃 张昊. 环介导恒温扩增技术(LAMP)及其在植物病毒检测中的应用. 中国农学通报. 2013, 29(21): 170-174 https://doi.org/10.11924/j.issn.1000-6850.2013-0240
Loop-mediated isothermal amplification(LAMP)and its application in detection of plant virus. Chinese Agricultural Science Bulletin. 2013, 29(21): 170-174 https://doi.org/10.11924/j.issn.1000-6850.2013-0240

参考文献

秦文韬 王忠跃 张昊 .环介导恒温扩增技术(LAMP)及其在植物病毒检测中的应用.中国农学通报 ,2013,29(第21期7月): 170-174

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