猕猴桃根中三萜类化合物的提取工艺研究

田娜 陈颖韬 吴文杰 张盛 刘硕谦

中国农学通报. 2013, 29(25): 184-189

中国农学通报 ›› 2013, Vol. 29 ›› Issue (25) : 184-189. DOI: 10.11924/j.issn.1000-6850.2013-0082
林学 园艺 园林

猕猴桃根中三萜类化合物的提取工艺研究

  • 田娜 陈颖韬 吴文杰 张盛 刘硕谦
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Optimization of Extraction Process for Triterpenoids from the Roots of Radix Actinidiae Chinensis

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摘要

【研究目的】对猕猴桃根中的三萜类化合物的提取工艺进行研究,获得最佳的提取工艺条件;【方法】采用单因素实验考察提取温度、乙醇体积分数、提取时间、料液比与提取次数对三萜类化合物得率的影响,并以正交试验进一步优化提取工艺条件;【结果】猕猴桃根三萜类物质的最优提取工艺条件为提取温度80℃,乙醇体积分数65%,提取时间1.5 h,料液比1:20 (g/mL),提取次数1次,在此工艺条件下,猕猴桃根三萜类物质的平均得率为6.7517%;【结论】该工艺应用于猕猴桃根中三萜类物质的提取简单可行,有较好的参考价值。

Abstract

【Objective】The study was aimed to optimize the extraction of the triterpenoids from the roots of Radix Actinidiae Chinensis and obtain the optimal extraction process. 【Methods】 The effect of extraction temperature, ethanol concentration (volume percent), extraction time, material-to-liquid ratio and the number of extraction times on the yield of triterpenoids is investigated through single factor tests and orthogonal tests. 【Results】The optimal extraction conditions were determined as follows: extraction temperature of 80℃, ethano concentration of 65%, extraction time of 1.5 h, material-to-liquid ratio of 1:20 (g/mL) and the number of extraction times was one time. Under the optimized extraction conditions, 6.7517% of the extraction yield of triterpenoids from Radix Actinidiae Chinensis was achieved. 【Conclusion】This optimized extraction process is simple, feasible and has a very good reference value.

关键词

正交试验

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orthogonal test

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田娜 陈颖韬 吴文杰 张盛 刘硕谦. 猕猴桃根中三萜类化合物的提取工艺研究. 中国农学通报. 2013, 29(25): 184-189 https://doi.org/10.11924/j.issn.1000-6850.2013-0082
Optimization of Extraction Process for Triterpenoids from the Roots of Radix Actinidiae Chinensis. Chinese Agricultural Science Bulletin. 2013, 29(25): 184-189 https://doi.org/10.11924/j.issn.1000-6850.2013-0082

参考文献

田娜 陈颖韬 吴文杰 张盛 刘硕谦 .猕猴桃根中三萜类化合物的提取工艺研究.中国农学通报 ,2013,29(第25期9月): 184-189

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