摘要：植物的脂肪酸合成主要在质体和内质网中进行，以甘油三酯（TAG）的形式在种子中贮藏。二酰甘油酰基转移酶(DGAT)催化TAG合成途径的最后阶段，是该途径的限速酶。本文通过生物信息学的方法，在全基因组水平上对大豆(Glycine max)DGAT基因家族进行了鉴定和分类，并分析了其基因结构、蛋白质结构域特征、系统发生及基因表达方式。研究结果表明：大豆中存在10个DGAT家族基因，分别属于DGAT1、DGAT2、DGAT3亚家族，分布在6条染色体上，其中DGAT1亚家族基因主要在茎端分生组织和绿色果荚中表达；DGAT3亚家族基因主要在根中表达；而DGAT2亚家族基因表达范围最广，主要在根瘤、叶、花和绿色果荚中表达。

Abstract: Triacylglycerol (TAG) is the main storage fat of the most oil seeds which is synthesized mainly in the plastid of plant. Diacylglycerolacyltransferase (DGAT) catalyzes the last step of triacylglycerolsynthetic pathway, which is a rate-limiting enzyme of thispathway. In this study, DGAT family genes of soybean are characterized in genome wide. Phylogenetic analysis characterized 3 classes of DGATgenes in soybean, which are belong to DGAT1, DGAT2 and DGAT3, and they distribute in 6 chromosomes of soybean genome.Whole genome expression analysis revealedthat DGAT1 subfamily genes mainly expressed in SAM and green pod while DGAT3 subfamily genes mainly in root, DGAT2 family genes broadly expressedin nodule, leaf, flower and green pod.