The experiment chose wild soft dates kiwi as a tender plant, and as a young leaves and stem section for explant, the wild soft tissue culture to the whole process dates kiwi, established a rapid and efficient wild soft dates kiwi regeneration system. The results showed that: the most suitable induction blade, stem section callus culture medium were respectively for MS+0.5 mg/L 6-BA+1.0 mg/L and NAA would MS+0.5 mg/L 6-BA+0.1 mg/L NAA; induction of callus in MS+0.6 mg/L 6-BA+0.05 mg/L NAA medium could be a very good differentiation in vitro shoot seedlings; bud induction multiple shoot clumps of culture medium was produced for MS+1.0 mg/L 6-BA+0.1 mg/L NAA; the better suitable for 1/2 MS root medium. In later experiment of callus found that auxin NAA could prevent callus from browning. In the callus differentiation, it was found that differentiated bud seeding to callus again, could improve the differentiation rate.