为了快速获得健康的葱兰幼苗，通过优化离体培养条件，建立葱兰高效的离体再生体系。利用葱兰叶片、鳞叶和顶芽作为外植体进行了愈伤组织和植株再生体系研究。结果表明，顶芽为最适外植体；丛生芽诱导和增殖的最适培养基为MS+TDZ 0.5 mg/L +NAA 0.1 mg/L，其次是MS+6-BA 0.5 mg/L+ NAA 0.1 mg/L；生根培养基为1/2MS+ IBA 0.5 mg/L，生根率为100%，为葱兰的工厂化生产提供技术参考。

The study aimed to establishment of regeneration system and getting healthy plantlet through optimizing in vitro culture condition. The regeneration system of Zephyranthes candida was established in this paper by using leaves, scale leaves and terminal bud as explants in vitro culture. The results showed that terminal bud was optimum explants for induction. The optimum medium for induction and proliferation of rosette buds was MS+ TDZ 0.5 mg/L+ NAA 0.1 mg/L, followed by MS+ 6-BA 0.5 mg/L + NAA 0.1 mg/L. The rooting medium was 1/2MS+ IBA 0.5 mg/L, the rooting rate reached 100%. The system proved a technological foundation for industrialized production of Zephyranthes candida.