为探索接种AM真菌对不同苗龄牡丹实生苗耐盐性的效应，将接种Glomus mosseae的1年生和2年生盆栽‘凤丹’牡丹实生苗，分别于不同浓度人工海水(0%、8%、16%和24%)下进行胁迫处理。结果表明，盐胁迫下接种G. mosseae的2年生‘凤丹’菌根侵染率显著高于1年生苗处理的，而叶片细胞膜透性则显著低于1年生苗处理的，盐胁迫下的增长量、根系活力、矿质元素N、P、K含量和耐盐系数均显著高于1年生苗的。1年生苗的菌根依赖性高于2年生苗；G. mosseae对1年生‘凤丹’植株的总干重、耐盐系数和N、P、K含量的菌根贡献率高于2年生苗的，且菌根贡献率随盐浓度的增加而增加。结论认为接种AM真菌对1年生牡丹苗的效应优于对2年生苗的，而且不同苗龄牡丹对菌根的依赖性不同。
Abstract
The purpose of this study was to estimate the effects of AM fungi on salt tolerance of the annual and biennial treepeony seedlings. The annual and biennial treepeony‘Fengdan’seedlings grown in pots were inoculated with or without Glomus mosseae, and then treated with artificial sea water (0%, 8%, 16% and 24%). The results showed that under salt stress mycorrhizal colonization of biennial treepeony seedlings inoculated with G. mosseae were significantly higher than that of annual treepeony seedlings, while the leaf relative conductivity was significantly lower than that of annual treepeony seedling. Under salt stress increment, root activity, N, P and K content, and index of salt tolerance were significantly higher in biennial treepeony seedlings than in annual treepeony seedlings. Mycorrhizal dependence of annual treepeony seedlings was higher than that of biennial treepeony seedlings. The total dry weight, index of salt tolerance, mycorrhizal contribution of N, P and K in annual peony seedlings inoculated with G. mosseae were higher than in biennial treepeony seedlings, with the increase of salt mycorrhizal contribution were increased. It was suggested that the effects of AM fungi on annual treepeony seedlings be superior to biennial treepeony seedlings, and treepeony with different growth stages showed different mycorrhizal dependence.
关键词
牡丹； 实生苗； AM真菌； 盐胁迫； 菌根依赖性
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Key words
Paeonia suffruticosa; seedling; arbuscular mycorrhizal fungi; salt stress; mycorrhizal dependence
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