基于液质联用检测柿子多酚成分的分离与鉴定
Study on the Separation and Identification of Polyphenols in Persimmon Using LC/MS Coupling Techniques
柿子中富含多酚类化合物，具有多种生理活性功能。为了获得柿子中的多酚组分，依次采用HP2MGL大孔吸附树脂、Sephadex LH-20凝胶多糖和ODS-A反相填料对柿子多酚进行分离纯化，先后获得了4种不同的柿子多酚组分。通过LC/MS的检测与鉴定，4种组分分别是没食子酸、儿茶素、表儿茶素和咖啡酸，这4种多酚纯度分别为没食子酸95.15%，儿茶素95.04%，表儿茶素87.47%，咖啡酸85.0%。本研究建立了一种从‘磨盘柿’中分离纯化柿子多酚组分的有效方法。
Persimmon is rich in natural phenolic compounds which have varieties of physiological functions. To separate and identify the polyphenols in persimmon, HP2MGL macroporous adsorption resin, Sephadex LH-20 polysaccharide gels and ODS-A reverse-phase media were employed orderly. Four different phenols in Diospyros kaki L. cv. Mopan, gallic acid, catechin, (-)-epicatechin and caffeine acid, were obtained, which were detected and identified using LC/MS method. The purity of gallic acid, catechin, (-)-epicatechin and caffeine acid was 95.15%, 95.04%, 87.47% and 85.0% respectively. An effective method of isolating and purifying phenolic components from Diospyros kaki L. cv. Mopan was established in this study.
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