研究电子束辐照对花粉萌发率的影响，为电子束辐射诱变育种提供实验基础。对‘京糯28’玉米花粉和陆地棉花粉进行不同剂量的电子束辐照，在液体培养基中对辐照的花粉进行培养，观察并统计二者的花粉萌发率。玉米‘京糯28’花粉在200~600 Gy辐射剂量范围内花粉萌发率均高于对照组，其中以600 Gy辐射剂量的萌发率最高；而陆地棉在300~1000 Gy辐射剂量范围内花粉的萌发率均低于对照组，但在50 h后对其花粉进行培养时，各辐照组的花粉萌发率均高于对照组。电子束辐照剂量在200~600 Gy范围内对‘京糯28’玉米花粉萌发率有促进作用，在300~1000 Gy范围内对陆地棉花粉萌发率有不同程度的抑制作用，但在一定程度上延长了陆地棉花粉生活力。

The aim was to study the effects of electron beam irradiation on pollen germination rate and provide the base for mutation breeding of electron beam irradiation. The maize pollen of ‘Beijing-waxy 28’ and pollen of Gossypium hirsutum L. were exposed in electron beam with different dose, the irradiated pollens were cultivated in a liquid medium, and then the pollen germination rates were observed and counted. The pollen germination rate of ‘Beijing-waxy 28’ was higher than that of the control group in 200-600 Gy radiation dose, with the highest germination rate at 600 Gy radiation dose. While the pollen germination rate of Gossypium hirsutum L. was lower than that of the control group in 300-1000 Gy radiation dose, but after the 50 h, the pollen germination rate was higher than that of the control groups. Electron beam radiation doses in the range of 200-600 Gy could facilitate the pollen germination rate of ‘Beijing-waxy 28’, and Electron beam radiation doses in the range of 300-1000 Gy could inhibit the pollen germination rate of Gossypium hirsutum L., but it could extend the activity of the Gossypium hirsutum L.pollen in a certain degree.