余甘子多糖提取物抗氧化活性研究
The Study on the Antioxidant Activity of Polysaccharide Extract from Phyllanthus emblica L.
利用超声提取-水提醇沉法从余甘子中提取可溶性多糖,多糖提取率达4.61%。提取物中多糖含量为44.62%。考查了余甘子多糖提取物的抗氧化活性,对Fenton反应羟自由基清除作用的IC50为0.38 mg/mL,最大清除率为80.3%,对光照核黄素超氧阴离子自由基清除作用的IC50为0.93 mg/mL,最大清除率为85.9%,对羟自由基诱发卵磷脂脂质过氧化损伤的IC50为0.39 mg/mL,最大抑制率为71.3%。实验结果表明余甘子富含活性多糖,余甘子多糖具有良好的抗氧化作用,最大清除作用与茶多酚相近,具有较好的研究价值。
The water soluble polysaccharide was extracted from phyllanthus emblica L. by the method of ultrasound-water extracting-alcohol precipitating. Polysaccharide extraction yield of 4.61% was obtained and the content of polysaccharide in the extract was reached to 44.62%. The antioxidant activity of the polysaccharide of phyllanthus emblica L. was investigated. The IC50 of scavenging effect on hydroxyl free radical generated by Fenton reaction was 0.38 mg/mL, the maximum scavenging rate was 80.3%. The IC50 of scavenging effect on superoxide radical generated by riboflavine photosensitization was 0.93 mg/mL, the maximum scavenging rate was 85.9 %. The IC50 of inhibition effect on lecithin lipid peroxidation induced by hydroxyl free radical was 0.39 mg/mL, the maximum inhibition rate was 71.3%. The results showed that phyllanthus emblica L. polysaccharides had good antioxidant activity, which was closed to tea polyphenols.
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