RNA干扰（RNAi）介导的植物抗病毒研究是近年来引起广泛关注的一项植物抗病毒基因工程策略。本文以GVA运动蛋白（MP）基因为模板扩增出了418 bp的基因片段，根据RNA干扰的基本原理，将目的片段正反向分别插入到载体pFGC5941内含子的两侧以便其转录过程中高效产生RNA发夹结构。经PCR扩增证明已成功构建以GVA MP基因为靶标的干扰载体pFGC5941-GVAFR，并成功转入农杆菌EHA105中，为后续探讨干扰载体的干涉效果奠定了基础。

The resistance induced by RNAi to plant virus is a new strategy for controlling the viruses in recent years. In this study, according to the basic principles of RNA interference, two fragments (418 bp) of GVA MP gene were amplified with PCR and inserted into both sides of intron of pFGC5941 in forwards and reverse way so that it produces hairpin RNA during translation efficiently. The construction of the interfering vector pFGC5941-GVAFR targeting to GVA MP gene was identified by PCR, and the vector was transferred into agrobacterium EHA105 successfully, it provided an important basis for further studying its RNAi effection.