水稻突变体的创制
Creation of Rice (Oryza sativa L.) Mutant
为功能基因组学研究提供较好的基础材料,利用0.75%甲基磺酸乙酯溶液和γ射线双重诱变处理‘晋恢21’M1、‘中花11’、LR001种子进行研究。结果表明,经过M2筛选,获得716份叶、茎、穗、粒等性状变异的突变体,突变频率较高,达1.79%,还获得一些如不分蘖、多分蘖、白化苗、mini-plant、稀粒、花器官等极具研究价值的突变类型。双重诱变可以获得各种类型的突变体,所有的突变类型在3种材料中均有出现,说明该技术可为功能基因组研究提供大量的实物材料。
To provide materials for the research of rice functional genomics, dry seeds of ‘Zhonghua11’and LR001 were induced with 60Co γ-ray, then these seeds and ‘Jinhui21’ M1 seed, induced by ethyl methane sulfonate (EMS) of 1%, were induced with 1% EMS. Through the screening to M2, seven hundred and sixteen mutants, including the changed organ of leaf, stem, panicles and grain, etc, were identified. Among these ones, some were of great value in research, such as no-tillering, more-tillering, albino, mini-plant, thin grains, flower organs, etc. More types of mutants were obtained, with a frequencies of mutation mean of 1.79%, and all emerged in the three varieties in double induction. This indicated that the double mutation technology might provide a lot of mutants for functional genomics research.
/
〈 |
|
〉 |