用液相色谱-串联质谱的方法建立鸡组织中地克珠利残留的检测方法，并考察地克珠利在鸡组织
中的消除规律。用乙腈和乙酸乙酯的混合溶液提取，60℃氮气吹干，乙腈溶解，过0.22 μm微孔滤膜后，
采用液相色谱-串联质谱分析。地克珠利在鸡肝中8~4000 μg/kg 范围内具有较好的线性关系（r2>0.99），
而在鸡肌肉中8~1200 μg/kg 范围内具有较好的线性关系（r2>0.99），检测限和定量限分别为0.5 μg/kg 和
0.8 μg/kg。鸡肝脏和肌肉中地克珠利的液相色谱串联质谱测定方法简单、灵敏度高，适用于鸡组织中地
克珠利残留量的检测。

Objective: we present a method based on liquid chromatography tandem mass spectrometry for determining residues of diclazuril in chicken tissues and investigate the depletion of diclazuril in chicken tissues. Method: The analyte was extracted by mixed solution of acetonitrile and ethyl-acetate, The extracts were dried by nitrogen gas, redissolved in acetonitrile, filtered through 0.22μm micropore film and directly injected into the LC–MS/MS system. Result: The calibration curves were linear (r2>0.99) in the range of 8~4000μg/kg in chicken liver and 8~1200μg/kg in chicken muscle. Limit of detection (LOD) and limit of quantitation (LOQ) were 0.5μg/kg and 0.8μg/kg. Conclusion: The method was simple and accurate enough to determine residues of diclazuril in chicken tissues.