甜瓜SSR引物在西葫芦中的通用性研究
Transferability of SSRs from Cucumis melo to Summer squash
本文分析了25对甜瓜SSR引物在西葫芦基因组中的可转移性,以期为西葫芦分子遗传育种研究提供分析工具。结果表明,约24%的甜瓜SSR引物可在西葫芦基因组中有效扩增。多态性分析发现6对引物中有4对在34份西葫芦种质中可以检测到多态性,等位变异数从3~6不等,平均为4。UPGMA 聚类分析表明,来自市场主栽F1的纯化材料和双单倍体材料之间多样性较差,说明现有栽培品种基因谱较窄;而从国家蔬菜种质资源库引进的材料多样性比较丰富,对这些材料的整理和重新评价有助于发掘新的基因源,为西葫芦杂交育种提供新材料。
Simple sequence repeat (SSR) is a popular tool in genetic studies. Currently, only a few SSR markers are available in summer squash (Cucurbita pepo L.), whereas plenty of SSR markers have been developed in its close relative Cucumis melo. In this study, 25 SSR primer pairs from Cucumis melo were tested in summer squash. Approximately 24% of them generated repeatable and clear products in summer squash, indicating a relative high homology between genomes of the two species. Of the transferable SSR markers, 6 primer pairs generated polymorphic products in 34 summer squash genotypes with a total of 16 alleles. Based on the SSR fingerprints, a relative low level of polymorphism was detected within the materials originated from F1 hybrid commonly seen in market while a high level of polymorphism was detected in those introduced from National Vegetable Ggermplasms Resource Center(NVGRC)
西葫芦;甜瓜;SSR;通用性
summer squash;cucumis melo ; SSRs; transferability
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