利用改良CTAB法提取木薯基因组DNA
Genomic DNA Extraction in Cassava by Modified CTAB Method
摘要:采用改良CTAB法提取木薯基因组DNA,该方法提取的基因组DNA琼脂糖凝胶电泳条带清晰,无拖尾现象。结果表明,DNA浓度在710.0 ng/μl~967.5 ng/μl 之间,OD260/OD280在1.73~1.92之间,该方法具有简便、快速、高效等特点,所提取的基因组DNA质量和纯度较高,适用于进一步的SSR、ISSR等分子标记分析。
Abstract: In this paper,genomic DNA extraction in cassava by modified CTAB method,the extracted products had the clear band and without tailing bands which could be observed through agar gel electrophoresis. The results show that the concentrations were between 710.0 ng/μl~967.5 ng/μl,and the values of OD260/OD280 were between 1.73~1.92. The modified CTAB method had characteristics of simple、rapid and effective,the genomic DNA extracted had high quality and purity,it could be used for SSR、 ISSR and other molecular markers.
关键词:改良CTAB
Key words: modified CTAB
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