RAPD和SRAP标记技术在苔藓植物亲缘关系研究中的比较分析
The Comparative Analysis on the Study of Genetic Relationship of Bryophytes Based on RAPD and SRAP Markers
利用RAPD和SRAP标记技术研究11种苔藓植物的亲缘关系,并进行比较分析。结果表明:RAPD扩增的多态性比率达100%,SRAP扩增的多态性比率达96.9%,两种标记结果均显示了很高的多态性比率。利用SPSS11.5软件对RAPD和SRAP扩增结果进行了聚类分析,两者均与形态学分类基本一致,但与形态学结果也有一定的差异,这种差异主要表现在科以上植物种类之间。同时,两种分子标记的聚类结果之间也存在一定的差异,主要是对大叶凤尾藓和小牛舌藓全缘亚种的划分。因此,RAPD和SRAP都有一定局限性,需要多种标记方法相互结合、相互印证,才能得出客观的结果。
RAPD and SRAP markers were applied to assess genetic relationship in 11 individuals of bryophytes . The results showed that both of tecniques dectected high proportion of polymorphic bands, which were 100% from RAPD and 96.9%from SRAP. By means of cluster analysis using SPSS11.5 software , the resultant dendrograms frrom RAPD and SRAP analyses were showed the generally correspondence with morphological classification except for some species among family. Comparing the two techniques, there were also some difference between SRAP and RAPD markers, mainly in the classfication of Fissidens grandifrons Brid .and Anomodon minor(Hedw.)Fuernr. ssp.integerrimus(Mitt.)Iwats.. Therefore, RAPD and SRAP had certain limitations, and we should be combined and confirmed with both in practice in order to obtain objective results.
苔藓
bryophytes
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