正交试验法对黑米黑色素的微波提取工艺研究
Study on Melanin Extraction from Black Rice by Microwave and Orthogonal Test
采用正交试验法,利用微波萃取技术,对黑米中黑色素的最佳提取工艺进行了深入研究。用分光光度法进行测定,以黑米黑色素的得率作为考查指标,对影响黑色素提取工艺的因素进行了优化研究。试验结果表明:乙醇浓度是影响黑米黑色素提取的最关键因子。优化得到黑色素的最佳提取工艺参数为:浸提剂乙醇浓度为80%、料液比1:20、微波作用功率为480W、微波作用时间为120s。在此实验条件下,黑米黑色素的得率达到3.2%,为先前文献报导的2.2倍。
The optimal conditions for microwave-assisted extraction of melanin from black rice were investigated by the orthogonal experiment. The content of melanin as research index in the extract was tested by spectrophotometry and the influencing factors of melanin were optimized. The results suggested that the ethanol concentration should be the statistically significant factor. The optimal parameters of the extraction process were as follows: ethanol concentration (80%), solid/liquid rate (1:20), microwave output power (480W) and microwave irradiation time (120s). Under the optimal experimental condition the extraction rate of melanin from black rice reached 3.2%, which was increased by a fctor of 2.2 compared with the former data.
正交试验
Orthogonal test
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