黄皮ISSR－PCR反应体系的优化

李开拓1,2,赵依杰3,钟凤林1,2,王江波1,2,施维属1,2,潘东明1,2

中国农学通报. 2009, 25(19): 37-41

中国农学通报 ›› 2009, Vol. 25 ›› Issue (19) : 37-41. DOI: 10.11924/j.issn.1000-6850.2009-0690
生物技术科学

黄皮ISSR－PCR反应体系的优化

  • 李开拓1,2,赵依杰3,钟凤林1,2,王江波1,2,施维属1,2,潘东明1,2
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Optimization of ISSR-PCR Reaction System in Wampee

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摘要

摘 要：采用单因素和正交试验对ISSR-PCR扩增黄皮基因组DNA的主要影响因子进行筛选和分析，建立了适宜于黄皮ISSR分析的扩增体系：20 μL的反应体系中含30 ng的模板DNA、1.5 U TaqDNA聚合酶、0.4 μmol/L引物、0.3mmol/L dNTPs以及引物(gA)8C的最佳退火温度为52.4℃。

Abstract

Abstract：The factors influencing ISSR-PCR to analyze the genetic divergence in Wampee were investigated by a single factor test and orthogonal design experiment.The results showed that a better amplification system of ISSR was obtained with the reation system comtaining 30ng template DNA,1.5U Taq DNA polymerase,0.4μmol/L primers,0.3mmol/L dNTPs in the total volume of 20μL.The optimized annealing temperature was 52.4℃ for primer (gA)8C.

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关键词：黄皮

/ ISSR-PCR；优化

Key words

Key words：Wampee

/ ISSR-PCR；Optimization

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李开拓1,2,赵依杰3,钟凤林1,2,王江波1,2,施维属1,2,潘东明1,2. 黄皮ISSR－PCR反应体系的优化. 中国农学通报. 2009, 25(19): 37-41 https://doi.org/10.11924/j.issn.1000-6850.2009-0690
Optimization of ISSR-PCR Reaction System in Wampee. Chinese Agricultural Science Bulletin. 2009, 25(19): 37-41 https://doi.org/10.11924/j.issn.1000-6850.2009-0690

参考文献

李开拓1,2，赵依杰3，钟凤林1,2，王江波1,2，施维属1,2，潘东明1,2 .黄皮ISSR－PCR反应体系的优化.中国农学通报 ,2009,25(19): 37-41

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