pCAMBIA2300-betA-BADH双价基因植物表达载体的构建

咸洋,,夏阳,张金文,庞彩红,李丽,毛秀红

中国农学通报. 2009, 25(9): 47-50

中国农学通报 ›› 2009, Vol. 25 ›› Issue (9) : 47-50. DOI: 10.11924/j.issn.1000-6850.2009-0146
生物技术科学

pCAMBIA2300-betA-BADH双价基因植物表达载体的构建

  • 咸洋,,夏阳,张金文,庞彩红,李丽,毛秀红
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Construction of plant expression vector of pCAMBIA2300-betA-BADH

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摘要

摘要：本实验以载体pCAMBIA2300-35s-OCS为基础通过分子克隆手段，将甜菜碱合成途径的两个关键酶基因，即编码胆碱脱氢酶（CDH）的betA基因和编码甜菜碱醛脱氧酶（BADH）的BADH基因及启动子和终止序列构建在同一植物表达载体上，得到pCAMBIA2300-betA-BADH双价植物表达载体。并将其导入根癌农杆菌GV3101和LBA4404，进一步用于双子叶植物的遗传转化。

Abstract

Abstract: The choline dehydrogenase（CDH）gene and the betaine aldehyde dehydrogenase（BADH）gene are the key genes of the glycine betaine’s synthesis pathway. They were cloned into the vector pCAMBIA2300-35s-OCS.They were tandem and have their respective promoters and terminators. The vector pCAMBIA2300-betA-BADH was transformed into the Agrobactrium tumefaciens GV3101, LBA4404 and will be used in the genetic transformation of the dicotyledons.

关键词

betA;BADH;酶切;PCR

Key words

betA;BADH;Enzyme cutting;PCR

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咸洋,,夏阳,张金文,庞彩红,李丽,毛秀红. pCAMBIA2300-betA-BADH双价基因植物表达载体的构建. 中国农学通报. 2009, 25(9): 47-50 https://doi.org/10.11924/j.issn.1000-6850.2009-0146
Construction of plant expression vector of pCAMBIA2300-betA-BADH. Chinese Agricultural Science Bulletin. 2009, 25(9): 47-50 https://doi.org/10.11924/j.issn.1000-6850.2009-0146

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