In order to make good use of SSR-PCR to identify the distant hybridization descendant of apricot, the orthogonal design was used to optimize SSR amplification system of Apricot hybrids in five factors at four levels respectively.A suitable SSR reaction system was established by range method, namely 20μl reaction system containing 1×buffer、2.0mM/L Mg2+、0.25mM/L dNTPs、0.25μM/L Primer、60ng/20μl DNA templet and 0.05 U/μl Taq polymerase.
Cai Shengwen, Sun Haoyuan, Yang Li, Wang Yuzhu, Lu Bingshe.
Optimization of SSR-PCR Reaction System of Apricot Distant Hybrids. Chinese Agricultural Science Bulletin. 2008, 24(7): 50-54 https://doi.org/10.11924/j.issn.1000-6850.20085929
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