【研究目的】季节性淹水胁迫是滩地杨树造林的主要限制因子，建立可靠的指标体系对美洲黑杨无性系耐涝性进行评价意义重大；【方法】以美洲黑杨XL-75、XL-77、XL-90、I-69及苏柳J172为对象，分析了其内部生理生化指标对淹水胁迫的响应、性状间相关性及其遗传变异；【结果】淹水胁迫下各无性系叶绿素含量、可溶性蛋白质含量、CAT活性在总体上表现为下降的趋势；I-69杨在淹水胁迫下MDA含量较对照明显上升，XL-75、XL-77、XL-90、J-172在淹水初期MDA含量较对照略有下降，在后期出现回升的趋势；淹水15d时，5个参试材料SOD活性与对照比较均明显下降，随着淹水胁迫程度加深，SOD活性表现为依次上升趋势，而POD变化则与SOD恰好相反，二者存在明显的补偿机制；淹水30d以内时，5个参试材料ADH活性均较对照显著提高，淹水45d时，I-69杨ADH活性较对照下降56.9%，而XL-75、XL-77、XL-90、J172分别较对照提高970.4%、279.1%、555.2%和461.5%；【结论】不同处理下Chl、SOD、CAT相对值与总生物量相对值呈极显著正相关，且其广义遗传力在0.5967~0.7743之间，表明其受遗传控制程度较高，可做为不同无性系耐涝性评价的可靠指标。
Abstract
【OBJECTIVE】The seasonal flooding stress was the main restriction factor for afforestation of Populus deltoides in beach land,the evaluation of flooding-tolerance ability through reliable properties was very important.【METHOD】The response of inner physiological characters,correlation among different characters and their genetic variation under flooding stress were studied objecting to Populus deltoides clones XL-75，XL-77，XL-90 and ck( I-69，Salix×jiangsuensis ‘J172’).【RESULT】Contents of chlorophyll, Soluble Protein and CAT activity of all clones under waterlogging stress were decreased totally compared to contrast. Content of MDA in I-69 was increased significantly compared with the contrast，Content of MDA in XL-75，XL-77，XL-90，J-172 in earlier time of waterlogging were decreased slightly compared with contrast and increased with sustain inundation. The SOD activity decreased obviously than CK in earlier time of waterlogging stress，but the POD activity rised obviously. With the waterlogging stress deepened，the SOD activity was rising one by one and the POD activity was declining one by one，both had obviously compensation on anti-oxygenation mechanism. Flooding within 30d，the ADH activity increased more obviously than CK. When flooding on 45d，the ADH activity of I-69 decreased 56.9% compared with CK，but the ADH activity of XL-75，XL-77，XL-90，J172 respectively increased 970.4%，279.1%，555.2% and 461.5% compared with CK.【CONCLUSION】The relative value of Chl，SOD，CAT had very remarkably positive correlation with the total biomass，and the heritabilities of Chl，SOD，CAT ranged from 0.5967 to 0.7743.It indicated that the variations of 3 traits were moderately or intensively inherited，and they could be used to evaluate the flooding-tolerance of populus deltoides clones.
关键词
淹水胁迫；过氧化产物；抗氧化酶；耐涝性；美洲黑杨
{{custom_keyword}} /
Key words
flooding stress;peroxidation products;antioxidase enzymes;flooding tolerance;Populus deltoides
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]刘晓忠,李建坤,王志霞,等.涝渍逆境下玉米叶片超氧物歧化酶和过氧化氢酶活性与抗涝性的关系[J].华北农学报,1995,10(3):29-32.
[2]Sarkar R K, Das A. Changes in antioxidative enzymes and antioxidants in relation to flooding tolerance in rice[J].Journal of Plant Biology,2000,27(3):307-311.
[3]蔡永萍,陶汉之,张玉琼.土壤渍水对小麦开花后叶片几种生理特性的影响[J].植物生理学通讯,2000,36(2):110-114.
[4]童富淡,胡家恕,邵爱萍.涝渍逆境下大麦叶片保护酶活性和膜脂过氧化作用的变化[J].浙江农业大学学报,1998,24(4):239-243.
[5]Neog B, Gogoi N, Baruah K K. Morpho-physiological changes associated with waterlogging in rice(Oryza sativa)[J].Indian Journal of Agricultural Sciences,2002,72(7):404-407.
[6]Seong R C, Kim J G, Nelson C J. Dry matter accumulation and leaf mineral contents as affected by excessive soil water in soybean.Korean Journal of Crop Science[J],1999,44(2):129-133.
[7]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2003.
[8]中国科学院上海植物生理研究所,上海市植物生理学会.现代植物生理学实验指导[M].北京:科学出版社,1999.
[9]惠红霞,许兴,李守明.宁夏干旱地区盐胁迫下枸杞光合生理特性及耐盐性研究.中国农学通报,2002,18(5):29-34.
[10]韩蕊莲,李丽霞,梁宗锁,等.干旱胁迫下沙棘膜脂过氧化保护体系研究[J].西北林学院学报,2002,17(4):1-5.
[11]唐罗忠,徐锡增,方升佐.土壤涝渍对杨树和柳树苗期生长及生理性状影响的研究[J].应用生态学报,1998,9(5):471-474.
[12]魏良民.几种旱生植物碳水化合物和蛋白质变化的研究[J].干旱区研究,1991,8(4):38-41.
[13]蔡志全,曹坤芳.遮荫下2种热带树苗叶片光合特性和抗氧化酶系统对自然降温的响应[J].林业科学,2004,40(1):47-51.
[14]Richard B, Couee I, Raymond P. Plant metabolism under hypoxia and anoxia[J].Plant Physiol Biochem,1994,32:1-10.
[15]Kennedy R A, Rumpho M E, Fox T C. Anaerobic metabolism in plants[J].Plant Physiol,1992,100:1-6.
[16]Umeda M, Uchimiya H.Differential transcript levels of genes associated with glycolysis and alcohol formation in rice plants(Oryza sativa L.) under submergence stress[J].Plant Physiol,1994,106:1015-1022.
[17]潘向艳,季孔庶,方彦.淹水胁迫下杂交鹅掌楸无性系几种酶活性的变化[J].西北林学院学报,2007,22(3):43-46.
[18]叶勇,卢昌义,谭凤仪.木榄和秋茄对水渍的生长与生理反应的比较[J].生态学报,2001,21(10):1654-1661.
[19]杨万年,林志福,任国邻,等.暗诱导油菜子叶衰老过程G-6-P脱氢酶和乙醇脱氢酶活性的变化[J].中国油料作物学报,2003,25(3):43-45.
[20]Musrur R, Anil G, James P W, et al. Effects of manipulation of pyruvate decarboxylase and alcohol dehydrogenase levels on the submergence tolerance of rice[J].Aust.J.Plant Physiol.,2001,28:1231-1241.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
